Ultrasensitive one-step rapid detection of ochratoxin A by the folding-based electrochemical aptasensor

2012 ◽  
Vol 753 ◽  
pp. 27-31 ◽  
Author(s):  
Jingjing Wu ◽  
Huaqin Chu ◽  
Zhanlong Mei ◽  
Yi Deng ◽  
Feng Xue ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rapid Detection ◽  
Electrochemical Aptasensor ◽  
One Step

Magnetic-fluorescent-targeting multifunctional aptasensorfor highly sensitive and one-step rapid detection of ochratoxin A

2015 ◽  
Vol 68 ◽  
pp. 783-790 ◽  
Author(s):  
Chengquan Wang ◽  
Jing Qian ◽  
Kan Wang ◽  
Kun Wang ◽  
Qian Liu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rapid Detection ◽  
Highly Sensitive ◽  
One Step

A novel label-free electrochemical aptasensor with one-step assembly process for rapid detection of lead (II) ions

2020 ◽  
Vol 320 ◽  
pp. 128326 ◽  
Author(s):  
Guojing Ran ◽  
Fengmei Wu ◽  
Xinyu Ni ◽  
Xuyan Li ◽  
Xusheng Li ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Assembly Process ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
One Step

Fabrication of electro-active nano-trans surfaces to design label free electrochemical aptasensor for ochratoxin A detection

2021 ◽  
Vol 379 ◽  
pp. 138172
Author(s):  
Maham Liaqat ◽  
Sara Riaz ◽  
Mian Hasnain Nawaz ◽  
Mihaela Badea ◽  
Akhtar Hayat ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Label Free ◽  
Electrochemical Aptasensor

scholarly journals Development and validation of a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in patient samples from Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Severino Jefferson Ribeiro da Silva ◽  
Keith Pardee ◽  
Udeni B. R. Balasuriya ◽  
Lindomar Pena
Keyword(s):  
Reverse Transcription ◽  
Rapid Detection ◽  
Quantitative Polymerase Chain Reaction ◽  
Rna Extraction ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Serum Samples ◽  
Low Resource ◽  
Loop Mediated Isothermal Amplification ◽  
One Step

AbstractWe have previously developed and validated a one-step assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of the Zika virus (ZIKV) from mosquito samples. Patient diagnosis of ZIKV is currently carried out in centralized laboratories using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR), which, while the gold standard molecular method, has several drawbacks for use in remote and low-resource settings, such as high cost and the need of specialized equipment. Point-of-care (POC) diagnostic platforms have the potential to overcome these limitations, especially in low-resource countries where ZIKV is endemic. With this in mind, here we optimized and validated our RT-LAMP assay for rapid detection of ZIKV from patient samples. We found that the assay detected ZIKV from diverse sample types (serum, urine, saliva, and semen) in as little as 20 min, without RNA extraction. The RT-LAMP assay was highly specific and up to 100 times more sensitive than RT-qPCR. We then validated the assay using 100 patient serum samples collected from suspected cases of arbovirus infection in the state of Pernambuco, which was at the epicenter of the last Zika epidemic. Analysis of the results, in comparison to RT-qPCR, found that the ZIKV RT-LAMP assay provided sensitivity of 100%, specificity of 93.75%, and an overall accuracy of 95.00%. Taken together, the RT-LAMP assay provides a straightforward and inexpensive alternative for the diagnosis of ZIKV from patients and has the potential to increase diagnostic capacity in ZIKV-affected areas, particularly in low and middle-income countries.

MGB-based one-step multiplex real-time PCR method for rapid detection of HPV

2013 ◽  
Vol 12 (3) ◽  
pp. 107-113 ◽  
Author(s):  
Jie Huang ◽  
Chun Gao ◽  
Xilai Ding ◽  
Shoufang Qu ◽  
Licheng Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
One Step ◽  
Pcr Method

An AC electrokinetics-based electrochemical aptasensor for the rapid detection of microRNA-155

2021 ◽  
pp. 113847
Author(s):  
Neil Adrian P. Ondevilla ◽  
Tak-Wah Wong ◽  
Nan-Yao Lee ◽  
Hsien-Chang Chang
Keyword(s):  
Rapid Detection ◽  
Electrochemical Aptasensor ◽  
Ac Electrokinetics

Comparison of carboxypeptidase Y and thermolysin for ochratoxin A electrochemical biosensing

2015 ◽  
Vol 7 (20) ◽  
pp. 8954-8960 ◽  
Author(s):  
Fatma Dridi ◽  
Mouna Marrakchi ◽  
Mohamed Gargouri ◽  
Joëlle Saulnier ◽  
Nicole Jaffrezic-Renault ◽  
...  
Keyword(s):  
Olive Oil ◽  
Ochratoxin A ◽  
Rapid Detection ◽  
Carboxypeptidase Y ◽  
Electrochemical Biosensing

Carboxypeptidase Y and thermolysin as sensing elements to develop an original biosensor for the direct and rapid detection of ochratoxin A in olive oil.

scholarly journals A One-Step, Real-Time PCR Assay for Rapid Detection of Rhinovirus

2010 ◽  
Vol 12 (1) ◽  
pp. 102-108 ◽  
Author(s):  
Duc H. Do ◽  
Stella Laus ◽  
Amy Leber ◽  
Mario J. Marcon ◽  
Jeanne A. Jordan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
One Step

Development of a colloidal gold strip for rapid detection of ochratoxin A with mimotope peptide

Food Control ◽  
2009 ◽  
Vol 20 (9) ◽  
pp. 791-795 ◽  
Author(s):  
Weihua Lai ◽  
Daniel Y.C. Fung ◽  
Xu Yang ◽  
Liu Renrong ◽  
Yonghua Xiong
Keyword(s):  
Ochratoxin A ◽  
Colloidal Gold ◽  
Rapid Detection
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