Miniaturized nucleic acid amplification systems for rapid and point-of-care diagnostics: A review

2012 ◽  
Vol 733 ◽  
pp. 1-15 ◽  
Author(s):  
Farhan Ahmad ◽  
Syed A. Hashsham
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Nucleic Acid Amplification ◽  
Point Of Care Diagnostics

scholarly journals A simple check valve for microfluidic point of care diagnostics

Lab on a Chip ◽  
2016 ◽  
Vol 16 (22) ◽  
pp. 4436-4444 ◽  
Author(s):  
C. S. Ball ◽  
R. F. Renzi ◽  
A. Priye ◽  
R. J. Meagher
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Check Valve ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Testing ◽  
Point Of Care Diagnostics

Laser cut microfluidic check valves enable staged reagent delivery, pumping, and point of care nucleic acid amplification testing.

Paper-based nucleic acid amplification tests for point-of-care diagnostics

The Analyst ◽  
2018 ◽  
Vol 143 (10) ◽  
pp. 2213-2234 ◽  
Author(s):  
Navjot Kaur ◽  
Bhushan J. Toley
Keyword(s):  
Nucleic Acid ◽  
Critical Review ◽  
Point Of Care ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Tests ◽  
Point Of Care Diagnostics

A critical review of paper-based nucleic acid amplification tests with a focus on integration and sequence of operations.

scholarly journals A Robust, Low-Cost Instrument for Real-time Colorimetric Isothermal Nucleic Acid Amplification

2021 ◽  
Author(s):  
Frank Myers ◽  
Brian Moffatt ◽  
Ragheb El Khaja ◽  
Titash Chatterjee ◽  
Gurmeet Marwaha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Diagnostic Assays ◽  
Isothermal Nucleic Acid Amplification ◽  
Point Of Care Diagnostics ◽  
Time Readout

The COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we present the design and construction of LARI along with thermal, optical, and assay performance characteristics. LARI can be produced for under $1500 and has broad applications in R&D, point-of-care diagnostics, and global health.

scholarly journals Nanomaterials for molecular signal amplification in electrochemical nucleic acid biosensing: recent advances and future prospects for point-of-care diagnostics

2020 ◽  
Vol 5 (1) ◽  
pp. 49-66 ◽  
Author(s):  
Léonard Bezinge ◽  
Akkapol Suea-Ngam ◽  
Andrew J. deMello ◽  
Chih-Jen Shih
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostics ◽  
Signal Amplification ◽  
Point Of Care ◽  
Future Prospects ◽  
Electrochemical Biosensing ◽  
Recent Advances ◽  
Point Of Care Diagnostics ◽  
Molecular Signal

This account reviews the major amplification strategies utilizing nanomaterials in electrochemical biosensing for robust and sensitive molecular diagnostics.

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