Electro membrane extraction of sodium diclofenac as an acidic compound from wastewater, urine, bovine milk, and plasma samples and quantification by high-performance liquid chromatography

2012 ◽  
Vol 722 ◽  
pp. 55-62 ◽  
Author(s):  
Saied Saeed Hosseiny Davarani ◽  
Ahmad Pourahadi ◽  
Saeed Nojavan ◽  
Mohammad Hossein Banitaba ◽  
Mahnaz Nasiri-Aghdam
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Bovine Milk ◽  
Membrane Extraction ◽  
Plasma Samples ◽  
Sodium Diclofenac ◽  
Acidic Compound

Validation of a Reversed-Phase High-Performance Liquid Chromatography Method With Fluorescence Detection for the Bioequivalence Study of Norfloxacin in Plasma Samples

2008 ◽  
Vol 30 (3) ◽  
pp. 341-346 ◽  
Author(s):  
Maria Bernadete Sousa Maia ◽  
Ismael Leite Martins ◽  
Demétrius Fernandes do Nascimento ◽  
Adriano Nunes Cunha ◽  
Francisco Evanir Gonçalves de Lima ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
High Performance ◽  
Bioequivalence Study ◽  
Reversed Phase ◽  
Plasma Samples ◽  
Chromatography Method ◽  
Liquid Chromatography Method

scholarly journals PHARMACOKINETIC PROFILE AND INCURRED ESOMEPRAZOLE SAMPLE STABILITY IN PLASMA USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY - PHOTODIODE ARRAY

2019 ◽  
pp. 214-219
Author(s):  
YAHDIANA HARAHAP ◽  
AHMAD FARIS ◽  
SUNARSIH .
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pharmacokinetic Profile ◽  
Clinical Samples ◽  
Plasma Samples ◽  
Elimination Phase ◽  
Sample Stability ◽  
Photodiode Array

Objective: Esomeprazole (ESO) is one of the proton-pump inhibitors and is used to treat gastroesophageal reflux. It is sensitive to low pH, heat,moisture, and oxidation, which often means that ESO in clinical samples is degraded at the time of storage, affecting analysis results. This study aimedto analyze the in vivo stability of ESO in subjects’ plasma samples by testing the incurred sample stability (ISS) of ESO in plasma following 7, 14, and28 days of storage at two concentrations close to Cmax and one concentration in the elimination phase.Methods: Samples were analyzed using high-performance liquid chromatography with a C18 column with detection at 300 nm using a photodiodearray detector. Lansoprazole was used as an internal standard.Results: The ESO pharmacokinetics profile in the plasma samples yielded the values of Cmax 704.57–1425.85 ng/mL; tmax is 2.25 h; and AUC0-t is2444 ng.h/mL. ISS testing of plasma samples values were 6.50%, 5.73%, and 4.57% on first Cmax concentration; 3.55%, 4.84%, and 3.68% on 2nd Cmaxconcentration; and 4.04%, 4.80%, and 4.98% on elimination phase concentration.Conclusion: ISS testing results of plasma samples from six healthy subjects who were administered doses of 40 mg of ESO stored for 28 days showedthat it fulfilled the acceptance criteria (<20%) of the 2011 EMEA Bioanalytical Guidelines with a %diff value in all incurred samples of 6.5%.

scholarly journals Optimization and Validation of a novel Nebulizer-assisted Liquid Phase Microextraction Followed by HPLC-DAD for Diazinon Analysis in Plasma Samples

2019 ◽  
Vol 9 (4) ◽  
pp. 221-232
Author(s):  
Reza Mohammadzaheri ◽  
Mehdi Ansari Dogaheh ◽  
Maryam Kazemipour ◽  
Kambiz Soltaninejad
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Liquid Phase ◽  
High Performance ◽  
Diode Array ◽  
Diode Array Detection ◽  
Plasma Samples ◽  
Liquid Phase Microextraction ◽  
Array Detection ◽  
Extracting Solvent

Background: Diazinon is among the most prevalently used broad-spectrum organophosphates insecticides. Diazinon toxicity depends on its blood concentration. The current study aimed to extract and determine diazinon in plasma samples using a new Nebulizer -Assisted Liquid-Phase Microextraction followed by High-Performance Liquid Chromatography with Diode-Array Detection (NALPME-HPLC-DAD).Methods: Several effective parameters, including the type and volume of extracting solvent, pH, surfactant, salt amount, and nebulizing, were evaluated and optimized to find the best condition for the extraction and determination of diazinon in plasma samples using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD). Additionally, the Plackett-Burman design was employed in preliminary experiments to screen the most appropriate parameters. Furthermore, we selected a central composite design to determine the best experimental conditions in NALPME-HPLC-DAD. Results: In an optimum condition, 412 μL of toluene (as extracting solvent) and nebulizing with nitrogen gas as dispersing and emulsification, sodium lauryl sulfate (2.8% w/v) and 100μL sodium chloride (1.5% w/v) in pH 8.1 were selected. The standard calibration curves for diazinon were linear with the concentration range of 0.5–4 µg/mL with a correlation coefficient of 0.9992. The Limit Of Detection (LOD) and Limit Of Quantification (LOQ) for diazinon were 0.123 µg/mL and 0.372 µg/mL, respectively.Conclusion: The proposed method was simple, accurate, precise, and sensitive for analyzing diazinon in the plasma samples. This method can be used for analyzing plasma diazinon concentrations in acute poisoning cases in clinical and forensic toxicology analyses.

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