Amperometric l-lactate biosensor based on screen-printed carbon electrode containing cobalt phthalocyanine, coated with lactate oxidase-mesoporous silica conjugate layer

2012 ◽  
Vol 714 ◽  
pp. 114-120 ◽  
Author(s):  
Takeshi Shimomura ◽  
Touru Sumiya ◽  
Masatoshi Ono ◽  
Tetsuji Ito ◽  
Taka-aki Hanaoka
Keyword(s):  
Mesoporous Silica ◽  
Cobalt Phthalocyanine ◽  
Carbon Electrode ◽  
Lactate Oxidase ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

scholarly journals The Scavenging Effect of Myoglobin from Meat Extracts toward Peroxynitrite Studied with a Flow Injection System Based on Electrochemical Reduction over a Screen-Printed Carbon Electrode Modified with Cobalt Phthalocyanine: Quantification and Kinetics

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 220
Author(s):  
Ioana Silvia Hosu ◽  
Diana Constantinescu-Aruxandei ◽  
Florin Oancea ◽  
Mihaela Doni
Keyword(s):  
Flow Injection ◽  
Linear Sweep Voltammetry ◽  
Differential Pulse ◽  
Injection System ◽  
Cobalt Phthalocyanine ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

The scavenging activity of myoglobin toward peroxynitrite (PON) was studied in meat extracts, using a new developed electrochemical method (based on cobalt phthalocyanine-modified screen-printed carbon electrode, SPCE/CoPc) and calculating kinetic parameters of PON decay (such as half-time and apparent rate constants). As reactive oxygen/nitrogen species (ROS/RNS) affect the food quality, the consumers can be negatively influenced. The discoloration, rancidity, and flavor of meat are altered in the presence of these species, such as PON. Our new highly thermically stable, cost-effective, rapid, and simple electrocatalytical method was combined with a flow injection analysis system to achieve high sensitivity (10.843 nA µM−1) at a nanomolar level LoD (400 nM), within a linear range of 3–180 µM. The proposed biosensor was fully characterized using SEM, FTIR, Raman spectroscopy, Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV), and Linear Sweep Voltammetry (LSV). These achievements were obtained due to the CoPc-mediated reduction of PON at very low potentials (around 0.1 V vs. Ag/AgCl pseudoreference). We also proposed a redox mechanism involving two electrons in the reduction of peroxynitrite to nitrite and studied some important interfering species (nitrite, nitrate, hydrogen peroxide, dopamine, ascorbic acid), which showed that our method is highly selective. These features make our work relevant, as it could be further applied to study the kinetics of important oxidative processes in vivo or in vitro, as PON is usually present in the nanomolar or micromolar range in physiological conditions, and our method is sensitive enough to be applied.

Biological Multilayer Films Assembled by Redox Polymer and HRP on Screen-Printed Carbon Electrode

2012 ◽  
Vol 571 ◽  
pp. 56-59
Author(s):  
Yu Fang Sha ◽  
Mei Zhao ◽  
Ming Quan Yang ◽  
Hai Xin Bai ◽  
Man Zhao
Keyword(s):  
Horseradish Peroxidase ◽  
Electrostatic Interaction ◽  
Electrode Surface ◽  
Multilayer Films ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Redox Polymer ◽  
Screen Printed Carbon Electrode ◽  
Positively Charged ◽  
Screen Printed

Biological multilayer films of redox polymer and horseradish peroxidase (HRP) were successfully assembled on a screen-printed carbon electrode using layer-by-layer (LBL) assembled method based on the electrostatic interaction. The screen-printed carbon electrode surface was modified by the positively charged redox polymer, and the negatively charged HRP by LBL method.

Screen-printed carbon electrode for the electrochemical detection of conjugated bilirubin

2021 ◽  
pp. 130574
Author(s):  
P.E. Resmi ◽  
Jeethu Raveendran ◽  
P.V. Suneesh ◽  
T. Ramanchandran ◽  
Bipin G Nair ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Conjugated Bilirubin ◽  
Screen Printed
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