Simultaneous determination of malachite green, brilliant green and crystal violet in grass carp tissues by a broad-specificity indirect competitive enzyme-linked immunosorbent assay

2011 ◽  
Vol 707 (1-2) ◽  
pp. 148-154 ◽  
Author(s):  
Yu-Dong Shen ◽  
Xing-Fei Deng ◽  
Zhen-Lin Xu ◽  
Yu Wang ◽  
Hong-Tao Lei ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Malachite Green ◽  
Immunosorbent Assay ◽  
Crystal Violet ◽  
Grass Carp ◽  
Brilliant Green ◽  
Enzyme Linked Immunosorbent Assay ◽  
Broad Specificity

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

2012 ◽  
Vol 6 (3) ◽  
pp. 767-774 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Greta Nölke ◽  
Jing Zhang ◽  
Lanlan Niu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Matrices

Determination of Residues of Three Triphenylmethane Dyes and Their Metabolites (Malachite Green, Leuco Malachite Green, Crystal Violet, Leuco Crystal Violet, and Brilliant Green) in Aquaculture Products by LC/MS/MS: First Action 2012.25

2013 ◽  
Vol 96 (5) ◽  
pp. 1152-1157 ◽  
Author(s):  
Dominique Hurtaud-Pessel ◽  
Pierrick Couëdor ◽  
Eric Verdon ◽  
Dawn Dowell
Keyword(s):  
Malachite Green ◽  
Crystal Violet ◽  
Brilliant Green ◽  
Triphenylmethane Dyes ◽  
Method Performance ◽  
Green Crystal ◽  
Leuco Crystal Violet ◽  
Aquaculture Products ◽  
Leuco Malachite Green

Abstract During the AOAC Annual Meeting held from September 30 to October 3, 2012 in Las Vegas, NV, the Expert Review Panel (ERP) on Veterinary Drug Residues reviewed data for the method for determination of residues of three triphenylmethane dyes and their metabolites (malachite green, leuco malachite green, crystal violet, leuco crystal violet, and brilliant green) in aquaculture products by LC/MS/MS, previously published in the Journal of Chromatography A 1218, 1632–1645 (2006). The method data were reviewed and compared to the standard method performance requirements (SMPRs) found in SMPR 2009.001, published in AOAC's Official Methods of Analysis, 19th Ed. (2012). The ERP determined that the data were acceptable, and the method was approved AOAC Official First Action. The method uses acetonitrile to isolate the analyte from the matrix. Then determination is conducted by LC/MS/MS with positive electrospray ionization. Accuracy ranged from 100.1 to 109.8% for samples fortified at levels of 0.5, 0.75, 1.0, and 2.0 μg/kg. Precision ranged from 2.0 to 10.3% RSD for the intraday samples and 1.9 to 10.6% for the interday samples analyzed over 3 days. The described method is designed to accurately operate in the analytical range from 0.5 to 2 μg/kg, where the minimum required performance limit for laboratories has been fixed in the European Union at 2.0 μg/kg for these banned substances and their metabolites. Upper levels of concentrations (1–100 μg/kg) can be analyzed depending on the different optional calibrations used.

Determination of malachite green, crystal violet, brilliant green and methylene blue by micro-cloud-point extraction and nonlinear laser wave-mixing detection interfaced to micellar capillary electrophoresis

2017 ◽  
Vol 9 (45) ◽  
pp. 6411-6419 ◽  
Author(s):  
Jean Sebastien Pradel ◽  
William G. Tong
Keyword(s):  
Methylene Blue ◽  
Capillary Electrophoresis ◽  
Malachite Green ◽  
Crystal Violet ◽  
Brilliant Green ◽  
Cloud Point Extraction ◽  
Detection Method ◽  
Wave Mixing ◽  
Laser Wave

A laser wave-mixing CE detection method is reported for separation and detection of malachite green (MG), crystal violet (CV), brilliant green (BG), methylene blue (MB), and the leuco-metabolites of MG and CV residues in aquacultures.

scholarly journals Simultaneous Determination of Florfenicol and Its Metabolite Florfenicol Amine in Swine Muscle Tissue by a Heterologous Enzyme-Linked Immunosorbent Assay

2009 ◽  
Vol 92 (3) ◽  
pp. 981-988 ◽  
Author(s):  
Pengjie Luo ◽  
Haiyang Jiang ◽  
Zhanhui Wang ◽  
Caimao Feng ◽  
Fangyang He ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Muscle Tissue ◽  
Immunosorbent Assay ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A sensitive and heterologous enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of florfenicol (FF) and its metabolite florfenicol amine (FFA) in swine muscle tissue was developed. FFA was conjugated to bovine serum albumin by a formaldehyde coupling method as an immunogen to immunize rabbits. FFA, thiamphenicol glycinate, and modified FF were conjugated to ovalbumin as coating antigens. The effect of different types of hapten heterology on the sensitivity and specificity of the ELISA was evaluated. Using FF glutaric anhydride ester as a coating hapten and antibody raised against modified FFA, an ELISA was developed that showed an IC50 value of 0.48 ng/mL. The antibody showed a cross-reactivity of 100 with FFA, 97 with FF, 6 with thiamphenicol, and a negligible value with chloramphenicol. From fortified swine muscle samples at levels of 4320 ng/g, the average recoveries of FF and FFA ranged from 58.2 to 96.8, with coefficients of variation less than 14. Analysis of incurred samples by the ELISA gave similar results to those by a previously developed liquid chromatographic method. The ELISA could be used as a rapid method for the simultaneous determination of FF and FFA in swine muscle tissue.

Simultaneous Determination of 13 Fluoroquinolone and 22 Sulfonamide Residues in Milk by a Dual-Colorimetric Enzyme-Linked Immunosorbent Assay

2013 ◽  
Vol 85 (4) ◽  
pp. 1995-1999 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Ross C. Beier ◽  
Haiyang Jiang ◽  
Yongning Wu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sulfonamide Residues
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