Determination of thebaine in water samples, biological fluids, poppy capsule, and narcotic drugs, using electromembrane extraction followed by high-performance liquid chromatography analysis

2011 ◽  
Vol 701 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Shahram Seidi ◽  
Yadollah Yamini ◽  
Akbar Heydari ◽  
Morteza Moradi ◽  
Ali Esrafili ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Water Samples ◽  
High Performance ◽  
Biological Fluids ◽  
Chromatography Analysis ◽  
Electromembrane Extraction ◽  
Performance Liquid Chromatography Analysis ◽  
Narcotic Drugs

scholarly journals Flavonoid Quantification in Onion by Spectrophotometric and High Performance Liquid Chromatography Analysis

HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 682-685 ◽  
Author(s):  
Kevin A. Lombard ◽  
Emmanuel Geoffriau ◽  
Ellen Peffley
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Correlation Coefficients ◽  
Cost Effective ◽  
Chromatography Analysis ◽  
Cost Effective Method ◽  
Performance Liquid Chromatography Analysis

Direct spectrophotometric determination of quercetin content in onions (Allium cepa L.) was investigated as a possible alternative to high-performance liquid chromatography (HPLC) analysis. Quercetin content in five onion varieties was monitored at 362 nm and quantified using simple spectrophotometric and HPLC methods. HPLC revealed that 3,4'-Qdg and 4'-Qmg comprised up to 93% of total flavonol content detected in the studied varieties. These major quercetin conjugates combined (3,4'-Qdg + 4'-Qmg) and total flavonol conjugates quantified by HPLC correlated closely with spectrophotometer values. Correlation coefficients were 0.96 (P < 0.0001) for 3,4'-Qdg + 4'-Qmg and 0.97 (P < 0.0001) for total flavonol conjugates in onion. Simple spectrophotometric procedure proved to be a valid, efficient, and cost-effective method for the quantification of total quercetin in onion. Chemical names used: quercetin-3,4'-O-diglucoside (3,4'-Qdg); quercetin-4'-O-glucoside (4'-Qmg).

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