Development of an ultra-high performance liquid chromatography analytical methodology for the profiling of olive (Olea europaea L.) pulp proteins

2011 ◽  
Vol 690 (1) ◽  
pp. 129-134 ◽  
Author(s):  
Clara Esteve ◽  
Carmen Del Río ◽  
M. Luisa Marina ◽  
M. Concepción García
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Olea Europaea ◽  
High Performance ◽  
Analytical Methodology ◽  
Olea Europaea L ◽  
Olea Europaéa

Free and hidden fumonisins in Argentinean raw maize samples

2020 ◽  
Vol 13 (1) ◽  
pp. 109-116
Author(s):  
P.S. Pok ◽  
V.A. García Londoño ◽  
S.P. Aransibia ◽  
S. Vicente ◽  
A.M. Pacín ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
European Commission ◽  
Alkaline Hydrolysis ◽  
High Performance ◽  
Fluorescence Detector ◽  
Analytical Methodology ◽  
Limits Of Detection

The concentrations of free fumonisins (FBs) and hydrolysed fumonisins (HFBs) were determined in 72 maize samples collected in 2017 from five Argentine provinces. The methodology for HFBs analysis consisted of alkaline hydrolysis followed by high performance liquid chromatography with a fluorescence detector (HPLC-FLD). The developed analytical methodology presented percentages of recovery greater than 74%. Limits of detection were 4.5, 13.0 and 12.9 μg/kg for HFB1, HFB2 and HFB3, respectively. Presence of FBs was found in 86% of the samples. In all cases, the concentration of total HFBs (after the hydrolysis treatment) was superior to the free FBs content (HFBs to FBs median ratio of 2.5), which indicates the presence of hidden fumonisins in Argentinean maize. 8% of the traditionally analysed samples exceeded the limit established by the European Commission for FB1 + FB2. When applying alkaline hydrolysis to the samples, 24% of them exceed this limit.

Liquid chromatography–mass spectrometry determination in plasma of maslinic acid, a bioactive compound from Olea europaea L.

2013 ◽  
Vol 141 (4) ◽  
pp. 4375-4381 ◽  
Author(s):  
Marta Sánchez-González ◽  
Glòria Lozano-Mena ◽  
M. Emília Juan ◽  
Andrés García-Granados ◽  
Joana M. Planas
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Olea Europaea ◽  
Bioactive Compound ◽  
Liquid Chromatography Mass Spectrometry ◽  
Maslinic Acid ◽  
Olea Europaea L ◽  
Chromatography Mass Spectrometry ◽  
Olea Europaéa

Vulcanization Chemistry. Sulfur, Nt-Butyl-2-Benzothiazole Sulfenamide Formulations Studied by High Performance Liquid Chromatography

1992 ◽  
Vol 65 (2) ◽  
pp. 488-501 ◽  
Author(s):  
A. B. Sullivan ◽  
Cynthia J. Hann ◽  
George H. Kuhls
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Material Balance ◽  
Side Reaction ◽  
Analytical Methodology ◽  
Wide Range ◽  
Kinetics Of ◽  
Vulcanization Process ◽  
Intermediate Concentration

Abstract The paper reports on the sulfur vulcanization chemistry of cis-polyisoprene formulations accelerated with N-t-butyl-2-benzothiazole sulfenamide. High Performance Liquid Chromatography (HPLC) is utilized to examine the kinetics of accelerator—sulfur disappearance and the formation—disappearance profiles of extra-network cure intermediates across the vulcanization process. Cure profile measurements are reported on three formulations covering a wide range of sulfur—accelerator ratios. Also unique aspects of the analytical methodology are highlighted. The observed cure-intermediate concentration profiles reinforce earlier theories about the chemical details of sulfurization and subsequent crosslinking. On all formulations, benzothiazole polysulfides (S1−S4) reach a maximum concentration at the scorch midpoint coincident with slope changes in reactant and product profiles. Although pendant-accelerator crosslink precursors are not measured directly, their presence is implied from material balance calculations. Also, the formation of a small amount of benzothiazole early in the scorch period is a new observation in sulfenamide acceleration. It suggests that accelerator hydrolysis is a competitive side reaction early in the vulcanization process.

scholarly journals The Evaluation of Prenylflavonoids Compounds in Romanian Beer

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Elena MUDURA ◽  
Adriana PAUCEAN ◽  
Maria TOFANA ◽  
Sonia SOCACI
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Analytical Methodology ◽  
Ms Detection ◽  
Brewing Process ◽  
Sample Purification

The evalution of prenylflavonoids from Romanian beer was done by an analytical methodology based on sample purification by centrifugation and subsequent analysis of the beer by high performance liquid chromatography with mass spectrometry MS/MS detection. In commercial beers less than 50 µg/l was found for XN, showing that a significant lost of this compound occurs during the brewing process. The isoxanthohumol is was found as principal prenylflavonoid in beer. The major concentration of prenylflavonoids was found in dark beer at 0.318 mg/l.

scholarly journals High Performance Liquid Chromatography versus Stacking-Micellar Electrokinetic Chromatography for the Determination of Potentially Toxic Alkenylbenzenes in Food Flavouring Ingredients

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 13
Author(s):  
Huynh N. P. Dang ◽  
Joselito P. Quirino
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Micellar Electrokinetic Chromatography ◽  
Reversed Phase ◽  
Food Samples ◽  
Electrokinetic Chromatography ◽  
Analytical Methodology

Alkenylbenzenes, including eugenol, methyleugenol, myristicin, safrole, and estragole, are potentially toxic phytochemicals, which are commonly found in foods. Occurrence data in foods depends on the quality of the analytical methodologies available. Here, we developed and compared modern reversed-phase high performance liquid chromatography (HPLC) and stacking-micellar electrokinetic chromatography (MEKC) methods for the determination of the above alkenylbenzenes in food flavouring ingredients. The analytical performance of HPLC was found better than the stacking-MEKC method. Compared to other HPLC methods found in the literature, our method was faster (total run time with conditioning of 15 min) and able to separate more alkenylbenzenes. In addition, the analytical methodology combining an optimized methanol extraction and proposed HPLC was then applied to actual food flavouring ingredients. This methodology should be applicable to actual food samples, and thus will be vital to future studies in the determination of alkenylbenzenes in food.

scholarly journals Lipidomic Profiling of the Olive (Olea europaea L.) Fruit towards Its Valorisation as a Functional Food: In-Depth Identification of Triacylglycerols and Polar Lipids in Portuguese Olives

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2555 ◽  
Author(s):  
Eliana Alves ◽  
Tânia Melo ◽  
Madalena P. Barros ◽  
M. Rosário M. Domingues ◽  
Pedro Domingues
Keyword(s):  
Liquid Chromatography ◽  
Molecular Species ◽  
Polar Lipid ◽  
Olea Europaea ◽  
Functional Food ◽  
Polar Lipids ◽  
Esi Ms ◽  
Olea Europaea L ◽  
Olive Pulp ◽  
Olea Europaéa

Olives (Olea europaea L.) are classic ingredients in the Mediterranean diet with well-known health benefits, but their lipid composition has not been fully addressed. In this work, we characterised triacylglycerol (TAG) and polar lipid profiles of the olive pulp while using a complementary methodological approach that was based on solid-phase extraction to recover the neutral lipid (NL) and the polar lipid-rich fractions. The TAG profile was analysed in the NL-fraction by C30 reversed-phase liquid chromatography (LC) and the polar lipid profile by normal-phase hydrophilic interaction liquid chromatography (HILIC), with both being coupled to electrospray ionization-mass spectrometry (ESI-MS) and ESI-MS/MS. This approach identified 71 TAG ions that were attributed to more than 350 molecular species, with fatty acyl chain lengths from C11:0 to C26:0, including different polyunsaturated acyl chains. The polar lipids included 107 molecular species that belonged to 11 lipid classes that comprised phospholipids, glyceroglycolipids, glycosphingolipids, and betaine lipids. In addition to polyunsaturated fatty acids, some of the phospholipids, glycolipids, and glycosphingolipids that were identified in the olive pulp have been described as biologically active molecules. Lipidomic phenotyping of the olive pulp has led to the discovery of compounds that will allow for a better assessment of its nutritional value and new applications of bioactive lipid components in this functional food.

scholarly journals Determination of ptaquiloside in cow's milk by HPLC-UV

2019 ◽  
Vol 40 (4) ◽  
pp. 1715 ◽  
Author(s):  
Debora da Silva Freitas Ribeiro ◽  
Kelly Moura Keller ◽  
Marília Martins Melo ◽  
Benito Soto-Blanco
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Health Risk ◽  
High Performance ◽  
Analytical Methodology ◽  
Potential Health ◽  
Potential Health Risk ◽  
Milk Samples ◽  
History Of

Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil.

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