A sensitive method for free amino acids analysis by liquid chromatography with ultraviolet and mass spectrometric detection using precolumn derivatization with diethyl ethoxymethylenemalonate: Application to the honey analysis

2010 ◽  
Vol 672 (1-2) ◽  
pp. 79-84 ◽  
Author(s):  
Riin Rebane ◽  
Koit Herodes
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Precolumn Derivatization ◽  
Diethyl Ethoxymethylenemalonate ◽  
Sensitive Method ◽  
Honey Analysis

A Rapid and Sensitive Method for Free Amino Acids in Nasal Feeding Nutrition by Liquid Chromatography with Liquid Extraction-Derivatization

2019 ◽  
Vol 83 (2) ◽  
pp. 293-297
Author(s):  
Pengyu Zhang ◽  
Wei Liu ◽  
Wei Li ◽  
Qingshuang Wang ◽  
Guilin Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Liquid Extraction ◽  
Sensitive Method

scholarly journals Determination ofβ-Cyano-L-alanine,γ-Glutamyl-β-cyano-L-alanine, and Common Free Amino Acids inVicia sativa(Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Cristina Megías ◽  
Isabel Cortés-Giraldo ◽  
Julio Girón-Calle ◽  
Javier Vioque ◽  
Manuel Alaiz
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Performance ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Diethyl Ethoxymethylenemalonate

A method for determination ofβ-cyano-L-alanine,γ-glutamyl-β-cyano-L-alanine and other free amino acids inVicia sativais presented. Seed extracts were derivatized by reaction with diethyl ethoxymethylenemalonate and analyzed by reverse-phase high-performance liquid chromatography. Calibration curves showed very good linearity of the response. The limit of detection and quantification was 0.15 and 0.50 μM, respectively. The method has high intra- (RSD = 0.28–0.31%) and interrepeatability (RSD = 2.76–3.08%) and remarkable accuracy with a 99% recovery in spiked samples. The method is very easy to carry out and allows for ready analysis of large number of samples using very basic HPLC equipment because the derivatized samples are very stable and have very good chromatographic properties. The method has been applied to the determination ofγ-glutamyl-β-cyano-L-alanine,β-cyano-L-alanine, and common free amino acids in eight wild populations ofV. sativafrom southwestern Spain.

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