Validation of an enzyme-linked immunosorbent assay screening for quinolones in egg, poultry muscle and feed samples

2009 ◽  
Vol 637 (1-2) ◽  
pp. 273-278 ◽  
Author(s):  
Giampiero Scortichini ◽  
Loredana Annunziata ◽  
Valeria Di Girolamo ◽  
Roberta Buratti ◽  
Roberta Galarini
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Feed Samples

Monoclonal Antibody–Based Sandwich Enzyme-Linked Immunosorbent Assay for Sensitive Detection of Prohibited Ruminant Proteins in Feedstuffs

2004 ◽  
Vol 67 (3) ◽  
pp. 544-549 ◽  
Author(s):  
FUR-CHI CHEN ◽  
Y.-H. PEGGY HSIEH ◽  
ROGER C. BRIDGMAN
Keyword(s):  
Immunosorbent Assay ◽  
Troponin I ◽  
Enzyme Linked Immunosorbent Assay ◽  
Spongiform Encephalopathy ◽  
Animal Products ◽  
Meat Meal ◽  
Heat Treated ◽  
Capture Antibody ◽  
Ruminant Meat ◽  
Feed Samples

Regulations aimed to control the epidemic of bovine spongiform encephalopathy have banned the use of certain animal products, i.e., ruminant meat and bone meals, in ruminant animal feeds. A sensitive enzyme-linked immunosorbent assay has been developed to detect prohibited bovine and ovine muscles in feedstuffs. The assay utilizes a pair of monoclonal antibodies (MAbs) against skeletal troponin I (TnI). MAb 5G9, specific to bovine and ovine TnI, was used as the capture antibody and the biotin-conjugated MAb 2G3, reacting to all heterologous TnI, was used as the detection antibody. Quantitative procedures were applied to samples containing 5, 0.5, and 0.05% (wt/wt) of heat-treated (132°C/2 bar, 2 h) bovine and ovine meat meals in three different feeds, coexisting with porcine, chicken, or turkey meat meal. The presence of these nonprohibited species did not affect the detection of bovine and ovine meat meals in the feed samples (P > 0.05). Quantitative determinations of extractable bovine and ovine TnI, with a detection limit of 5.0 and 4.0 ng/ml, respectively, were achieved when the matching feed matrixes were used in the calibration curves. This new assay provides a rapid and reliable way to detect animal protein products containing a trace amount of bovine or ovine muscle tissue in feedstuffs.

Development of a sensitive monoclonal antibody-based ELISA for the determination of a β-adrenergic agonist brombuterol in swine meat, liver and feed samples

2016 ◽  
Vol 8 (38) ◽  
pp. 6941-6948 ◽  
Author(s):  
Yingying Zhao ◽  
Danni Jiang ◽  
Kang Wu ◽  
Hong Yang ◽  
Haijing Du ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adrenergic Agonist ◽  
Feed Samples

A sensitive enzyme-linked immunosorbent assay (ELISA) based on a newly prepared monoclonal antibody (mAb) for the determination of a β-adrenergic agonist brombuterol in swine meat, liver and feed samples was developed.

scholarly journals A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples

Toxins ◽  
2015 ◽  
Vol 7 (10) ◽  
pp. 4216-4231 ◽  
Author(s):  
Xian Zhang ◽  
Xin Wang ◽  
Mengjiao Sun ◽  
Xiaofeng Zhang ◽  
Houhui Song ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Magnetic Nanoparticle ◽  
Enzyme Linked Immunosorbent Assay ◽  
Feed Samples

scholarly journals Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples

Toxins ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 48 ◽  
Author(s):  
Aline Omori ◽  
Elisabete Ono ◽  
Melissa Hirozawa ◽  
Igor de Souza Suguiura ◽  
Elisa Hirooka ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Health Hazard ◽  
Fusarium Verticillioides ◽  
Cross Reactivity ◽  
Poultry Feed ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Feed Samples

Fumonisins are a group of toxic secondary metabolites that are produced by Fusarium verticillioides which are associated with poultry health hazard and great economic losses. The objective of the present study was to develop an immunological method to detect F. verticillioides in poultry feed samples. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a polyclonal antibody against 67 kDa protein of the F. verticillioides 97K exoantigen was developed to detect this fungus. Antibody anti-67 kDa protein showed cross-reactivity against F. graminearum (2–7%) and F. sporotrichioides (10%), but no or low cross-reactivity against Aspergillus sp. and Penicillium sp. exoantigens. The detection limit for the 67 kDa protein of F. verticillioides was 29 ng/mL. Eighty-one poultry feed samples were analyzed for Fusarium sp. count, 67 kDa protein of F. verticillioides and fumonisin concentrations. Eighty of the 81 feed samples (98.6%) showed Fusarium sp. contamination (mean 6.2 x 104 CFU/g). Mean 67 kDa protein and fumonisin concentration in the poultry feed samples was 21.0 µg/g and 1.02 µg/g, respectively. The concentration of 67 kDa protein, as determined by ic-ELISA correlated positively (p < 0.05) with fumonisin levels (r = 0.76). These results suggest that this ic-ELISA has potential to detect F. verticillioides and predict fumonisin contamination in poultry feed samples.

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