Tracking of mercury ions in living cells with a fluorescent chemodosimeter under single- or two-photon excitation

2007 ◽  
Vol 597 (2) ◽  
pp. 306-312 ◽  
Author(s):  
Zhou-Jun Lu ◽  
Pei-Nan Wang ◽  
Yu Zhang ◽  
Ji-Yao Chen ◽  
Shen Zhen ◽  
...  
Keyword(s):  
Living Cells ◽  
Mercury Ions ◽  
Two Photon ◽  
Photon Excitation ◽  
Fluorescent Chemodosimeter ◽  
Two Photon Excitation

scholarly journals Two-photon excitation in living cells induced by low-power cw laser beams

1996 ◽  
Author(s):  
Karsten Koenig ◽  
Tatiana B. Krasieva ◽  
Yagang Liu ◽  
Michael W. Berns ◽  
Bruce J. Tromberg
Keyword(s):  
Low Power ◽  
Living Cells ◽  
Laser Beams ◽  
Two Photon ◽  
Cw Laser ◽  
Photon Excitation ◽  
Two Photon Excitation

Determination of acceptor-to-donor cross section ratio for two-photon excitation in living cells

2016 ◽  
Author(s):  
Zexian Hou ◽  
Yuhua Wang ◽  
Liqin Zheng ◽  
Tongsheng Chen ◽  
Hongqin Yang ◽  
...  
Keyword(s):  
Cross Section ◽  
Living Cells ◽  
Cross Section Ratio ◽  
Two Photon ◽  
Section Ratio ◽  
Photon Excitation ◽  
Two Photon Excitation

Fluorescence detection of protoporphyrin IX in living cells: a comparative study on single- and two-photon excitation

2008 ◽  
Vol 13 (2) ◽  
pp. 024014 ◽  
Author(s):  
Sijia Lu ◽  
Ji-Yao Chen ◽  
Yu Zhang ◽  
Jiong Ma ◽  
Pei-Nan Wang ◽  
...  
Keyword(s):  
Comparative Study ◽  
Fluorescence Detection ◽  
Protoporphyrin Ix ◽  
Living Cells ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Real-time monitoring of intracellular nitric oxide using a long-wavelength-emitting probe via one-photon or two-photon excitation

2019 ◽  
Vol 7 (11) ◽  
pp. 3246-3252 ◽  
Author(s):  
Changquan Tang ◽  
Mingxue Wang ◽  
Xiaoying Shang ◽  
Xueyuan Chen ◽  
Deguang Huang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Long Wavelength ◽  
Two Photon ◽  
Turn On ◽  
Photon Excitation ◽  
Endogenous Nitric Oxide ◽  
Two Photon Excitation

A long-wavelength-emitting fluorescent turn-on probe is developed for monitoring exogenous or endogenous nitric oxide in living cells.

Thiol-Capped CdTe Quantum Dots with Two-Photon Excitation for Imaging High Autofluorescence Background Living Cells

2008 ◽  
Vol 19 (4) ◽  
pp. 615-621 ◽  
Author(s):  
Tao Wang ◽  
Ji-Yao Chen ◽  
Shen Zhen ◽  
Pei-Nan Wang ◽  
Chang-Chun Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Living Cells ◽  
Cdte Quantum Dots ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-Photon Excitation Microscopy in Cellular Biophysics

1996 ◽  
Vol 54 ◽  
pp. 276-277
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Living Cells ◽  
Input Power ◽  
Photon Absorption ◽  
Infrared Light ◽  
Focal Volume ◽  
Excitation Light ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10 5 limits the average input power to less than 10 mW, only slightly greater than the power normally used in confocal microscopy.Three properties TPEM give this method a tremendous advantage over conventional optical sectioning microscopies for the study of thick samples: 1) The excitation is limited to the focal volume because of the intensity-squared dependence of the two-photon absorption. This inherent localization provides three-dimensional resolution and eliminates background equivalent to an ideal confocal microscope without requiring a confocal spatial filter, whose absence enhances fluorescence collection efficiency. Confinement of excitation to the focal volume also minimizes photobleaching and photo damage - the ultimate limiting factors in fluorescence microscopy of living cells and tissues. 2) The two-photon technique allows imaging of UV fluorophores with conventional visible light optics in both the scanning and imaging systems, because both the red excitation light (~700 nm) and the blue fluorescence (>400 nm) are within the visible spectrum. 3) Red or infrared light is far less damaging to most living cells and tissues than bluer light because fewer biological molecules absorb at the higher wavelengths. Longer wavelength excitation also reduces scattering of the incident light by the specimen, thus allowing more of the input power to reach the focal plane. This relative transparency of biological specimens to 700 nm light permits deeper sectioning, since both absorbance and scattering are reduced.

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