Oxidative status of human low density lipoprotein isolated by anion-exchange high-performance liquid chromatography—Assessment by total hydroxyoctadecadienoic acid, 7-hydroxycholesterol, and 8-iso-prostaglandin F2α

2007 ◽  
Vol 585 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Soichi Kitano ◽  
Yasukazu Yoshida ◽  
Katsumi Kawano ◽  
Nozomu Hibi ◽  
Etsuo Niki
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Prostaglandin F2α ◽  
Oxidative Status ◽  
Low Density ◽  
Hydroxyoctadecadienoic Acid

tHcy Detected by High Performance Liquid Chromatography will be a Dangerous Factor for the Invasion of Occlusive Vascular Disease

2012 ◽  
Vol 268-270 ◽  
pp. 1644-1647
Author(s):  
Hong Yan Zhou ◽  
Wen Song Zhang ◽  
Ji Long Hao ◽  
Shuang Wang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Diabetic Retinopathy ◽  
Liquid Chromatography ◽  
Folic Acid ◽  
High Performance ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Occlusive Vascular Disease ◽  
Plasma Thcy

Objective:To study the clinical application of high performance liquid chromatography（HPLC)、Radioimmunodetection and Automatic biochemical Analyzer on the detection of plasma total homocysteine(tHcy)、folic acid（FA）、VitB12、Triglyceride（TG）、total cholesterol(Ch)、high density lipoprotein cholesferol(HDL-C) and low density lipoprotein cholestorol(LDL-C).To manifest mechanism of the increasing of tHcy leading to diabetic retinopathy.Methods:The levels of plasma tHcy 、folic acid、VitB12、blood sugar、 blood pressure and blood lipid were detected by the instrumentations above in diabetic retinopathy cases.Results:The levels of plasma tHcy in diabetic retinopathy group were much higher than that in the control group.Conclusions:We discovered biomarkers of diabetic retinopathy.HPLC method can be used well for the detection of tHcy.

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