Ultraviolet absorbance detection of colchicine and related alkaloids on a capillary electrophoresis microchip

2006 ◽  
Vol 572 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Qin Lu ◽  
Christine L. Copper ◽  
Greg E. Collins
Keyword(s):  
Capillary Electrophoresis ◽  
Ultraviolet Absorbance ◽  
Absorbance Detection ◽  
Electrophoresis Microchip

Investigation of photochemical reactions of saccharides during direct ultraviolet absorbance detection in capillary electrophoresis

2015 ◽  
Vol 1388 ◽  
pp. 259-266 ◽  
Author(s):  
Thomas Schmid ◽  
Markus Himmelsbach ◽  
James D. Oliver ◽  
Marianne Gaborieau ◽  
Patrice Castignolles ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Photochemical Reactions ◽  
Ultraviolet Absorbance ◽  
Absorbance Detection

Measurement of urinary oxalate and citrate by capillary electrophoresis and indirect ultraviolet absorbance

1995 ◽  
Vol 41 (9) ◽  
pp. 1297-1301 ◽  
Author(s):  
R P Holmes
Keyword(s):  
Capillary Electrophoresis ◽  
Signal To Noise Ratio ◽  
Rapid Analysis ◽  
Urinary Oxalate ◽  
Signal To Noise ◽  
Ultraviolet Absorbance ◽  
Absorbance Detection ◽  
Detectable Concentration ◽  
Time Accuracy ◽  
Concentration Signal

Abstract We describe a method for measuring urinary oxalate and citrate with capillary electrophoresis (CE) and indirect ultraviolet absorbance detection. Sample preparation is minimal, requiring an acidification, brief centrifugation, and dilution. The method is rapid, with oxalate and citrate having mean migration times of 4.02 and 4.50 min, respectively. The minimal detectable concentration (signal-to-noise ratio of 7) of both oxalate and citrate in urine was 7 mg/L. Total imprecisions (CV) were 1.2-5.6% for three urine samples with oxalate and citrate concentrations of 8-60 mg/L and 80-860 mg/L, respectively. The recovery of added oxalate ranged from 94% to 101%. Results of CE analyses agreed well with enzymatic determinations of oxalate and citrate. Rapid analysis time, accuracy, and reproducibility make this procedure well suited for routine urinary oxalate and citrate determinations.

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