A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanoparticles embedded in silica sol–gel network on gold modified electrode

2005 ◽  
Vol 553 (1-2) ◽  
pp. 196-200 ◽  
Author(s):  
Junwei Di ◽  
Chunping Shen ◽  
Shaohua Peng ◽  
Yifeng Tu ◽  
Shujin Li
2002 ◽  
Vol 74 (9) ◽  
pp. 2217-2223 ◽  
Author(s):  
Jianbo Jia ◽  
Bingquan Wang ◽  
Aiguo Wu ◽  
Guangjin Cheng ◽  
Zhuang Li ◽  
...  

2009 ◽  
Vol 24 (7) ◽  
pp. 2273-2276 ◽  
Author(s):  
Liu Deng ◽  
Lihua Zhang ◽  
Li Shang ◽  
Shaojun Guo ◽  
Dan Wen ◽  
...  

2018 ◽  
Vol 47 (9) ◽  
pp. 2925-2932 ◽  
Author(s):  
J. Brendlé

The sol–gel process involving hydrolysis and condensation reactions is an attractive way to form siloxane based hybrid materials since it is a one-step method performed under mild conditions.


1975 ◽  
Vol 23 (3) ◽  
pp. 200-207 ◽  
Author(s):  
D M Boorsma ◽  
G L Kalsbeek

In this study we compared horseradish peroxidase (HRP)-labeled rabbit antihuman immunoglobulin G (IgG) conjugates, prepared by a one-step and a two-step method. Glutaraldehyde was used as a cross-linking agent. Two methods were used for removing unconjugated HRP: Sephadex G-200 gel chromatography and ammonium sulfate precipitation. The conjugates were characterized immunologically, immunochemically and enzymatically. The immunohistoenzymic properties of the conjugates were tested on unfixed cryostat sections of the skin of patients with chronic discoid lupus erythematosus. The influence of the presence of unconjugated HRP and unconjugated IgG was studied. Optimal results were obtained with conjugates prepared by a two-step method. Removing unconjugated HRPimproved the immunohistoenzymic properties of the conjugates. Conjugated and unconjugated IgG could be separated by Sephadex G-200 gel chromatography.


2012 ◽  
Vol 535-537 ◽  
pp. 2563-2566 ◽  
Author(s):  
Yu Ma ◽  
Hye Ryeon Lee ◽  
Toshinori Tsuru

The synthesis of hydrophobic sol by one-step sol-gel process ammonia catalyzed was investigated. The water molar ratio and catalyst molar ratio were discussed to prevent phase segregation during the hydrolysis and co-condensation of the organic and inorganic precursors. The reactant system with water molar ratio 70 could make the reaction rate of MTES slightly less than that of TEOS, so that the hydrolysis - condensation – gelling reaction with MTES and TEOS as co-precursors could be synchronously. With the increase of the MTES/TEOS molar ratio, the reaction rate of the silica sol preparation decreased, the hydrophobicity of the sol and gel increased as the molar ratio MTES/TEOS change from 0.5~4. But the hydrophobicity of the methyl-modified silica sol and gel prepared with pure MTES in the sol-gel process were slightly lower than that of the methyl-modified silica sol and gel prepared with MTES and TEOS as co-precursors.


Talanta ◽  
2011 ◽  
Vol 86 ◽  
pp. 400-407 ◽  
Author(s):  
Palanisamy Kannan ◽  
Hailan Chen ◽  
Victor Tswen-Wen Lee ◽  
Dong-Hwan Kim

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