A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanoparticles embedded in silica sol–gel network on gold modified electrode

2005 ◽  
Vol 553 (1-2) ◽  
pp. 196-200 ◽  
Author(s):  
Junwei Di ◽  
Chunping Shen ◽  
Shaohua Peng ◽  
Yifeng Tu ◽  
Shujin Li
Keyword(s):  
Gold Nanoparticles ◽  
Horseradish Peroxidase ◽  
Modified Electrode ◽  
Sol Gel ◽  
Silica Sol ◽  
Third Generation ◽  
Step Method ◽  
One Step ◽  
Gel Network

A Method to Construct a Third-Generation Horseradish Peroxidase Biosensor:  Self-Assembling Gold Nanoparticles to Three-Dimensional Sol−Gel Network

2002 ◽  
Vol 74 (9) ◽  
pp. 2217-2223 ◽  
Author(s):  
Jianbo Jia ◽  
Bingquan Wang ◽  
Aiguo Wu ◽  
Guangjin Cheng ◽  
Zhuang Li ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Horseradish Peroxidase ◽  
Sol Gel ◽  
Three Dimensional ◽  
Third Generation ◽  
Self Assembling ◽  
Gel Network

Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol–gel network with gold nanoparticles

2009 ◽  
Vol 24 (7) ◽  
pp. 2273-2276 ◽  
Author(s):  
Liu Deng ◽  
Lihua Zhang ◽  
Li Shang ◽  
Shaojun Guo ◽  
Dan Wen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Sol Gel ◽  
Gel Network

Organic–inorganic hybrids having a talc-like structure as suitable hosts to guest a wide range of species

2018 ◽  
Vol 47 (9) ◽  
pp. 2925-2932 ◽  
Author(s):  
J. Brendlé
Keyword(s):  
Hybrid Materials ◽  
Sol Gel ◽  
Step Method ◽  
Mild Conditions ◽  
Condensation Reactions ◽  
Wide Range ◽  
Sol Gel Process ◽  
Hydrolysis And Condensation ◽  
One Step

The sol–gel process involving hydrolysis and condensation reactions is an attractive way to form siloxane based hybrid materials since it is a one-step method performed under mild conditions.

scholarly journals A comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method.

1975 ◽  
Vol 23 (3) ◽  
pp. 200-207 ◽  
Author(s):  
D M Boorsma ◽  
G L Kalsbeek
Keyword(s):  
Horseradish Peroxidase ◽  
Lupus Erythematosus ◽  
Cross Linking ◽  
Discoid Lupus Erythematosus ◽  
Gel Chromatography ◽  
Ammonium Sulfate Precipitation ◽  
Step Method ◽  
One Step ◽  
Cryostat Sections ◽  
Unfixed Cryostat

In this study we compared horseradish peroxidase (HRP)-labeled rabbit antihuman immunoglobulin G (IgG) conjugates, prepared by a one-step and a two-step method. Glutaraldehyde was used as a cross-linking agent. Two methods were used for removing unconjugated HRP: Sephadex G-200 gel chromatography and ammonium sulfate precipitation. The conjugates were characterized immunologically, immunochemically and enzymatically. The immunohistoenzymic properties of the conjugates were tested on unfixed cryostat sections of the skin of patients with chronic discoid lupus erythematosus. The influence of the presence of unconjugated HRP and unconjugated IgG was studied. Optimal results were obtained with conjugates prepared by a two-step method. Removing unconjugated HRPimproved the immunohistoenzymic properties of the conjugates. Conjugated and unconjugated IgG could be separated by Sephadex G-200 gel chromatography.

Study on Preparation and Hydrophobicity of MTES Derived Silica Sol and Gel

2012 ◽  
Vol 535-537 ◽  
pp. 2563-2566 ◽  
Author(s):  
Yu Ma ◽  
Hye Ryeon Lee ◽  
Toshinori Tsuru
Keyword(s):  
Reaction Rate ◽  
Sol Gel ◽  
Phase Segregation ◽  
Silica Sol ◽  
Molar Ratio ◽  
Modified Silica ◽  
Sol Gel Process ◽  
One Step ◽  
Inorganic Precursors

The synthesis of hydrophobic sol by one-step sol-gel process ammonia catalyzed was investigated. The water molar ratio and catalyst molar ratio were discussed to prevent phase segregation during the hydrolysis and co-condensation of the organic and inorganic precursors. The reactant system with water molar ratio 70 could make the reaction rate of MTES slightly less than that of TEOS, so that the hydrolysis - condensation – gelling reaction with MTES and TEOS as co-precursors could be synchronously. With the increase of the MTES/TEOS molar ratio, the reaction rate of the silica sol preparation decreased, the hydrophobicity of the sol and gel increased as the molar ratio MTES/TEOS change from 0.5~4. But the hydrophobicity of the methyl-modified silica sol and gel prepared with pure MTES in the sol-gel process were slightly lower than that of the methyl-modified silica sol and gel prepared with MTES and TEOS as co-precursors.

Highly sensitive amperometric detection of bilirubin using enzyme and gold nanoparticles on sol–gel film modified electrode

Talanta ◽  
2011 ◽  
Vol 86 ◽  
pp. 400-407 ◽  
Author(s):  
Palanisamy Kannan ◽  
Hailan Chen ◽  
Victor Tswen-Wen Lee ◽  
Dong-Hwan Kim
Keyword(s):  
Gold Nanoparticles ◽  
Modified Electrode ◽  
Sol Gel ◽  
Amperometric Detection ◽  
Highly Sensitive ◽  
Gel Film
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