Peptides analysis in blood plasma using on-line system of supported liquid membrane and high-performance liquid chromatography

2005 ◽  
Vol 553 (1-2) ◽  
pp. 9-14 ◽  
Author(s):  
Anna Drapała ◽  
Jan Åke Jönsson ◽  
Piotr Wieczorek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Blood Plasma ◽  
High Performance ◽  
Liquid Membrane ◽  
Supported Liquid Membrane ◽  
Line System ◽  
On Line ◽  
In Blood Plasma

scholarly journals Plasma radio-metabolite analysis of PET tracers for dynamic PET imaging: TLC and autoradiography

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fiona Li ◽  
Justin W. Hicks ◽  
Lihai Yu ◽  
Lise Desjardin ◽  
Laura Morrison ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Blood Plasma ◽  
Kinetic Parameters ◽  
High Performance ◽  
The Body ◽  
Accurate Estimation ◽  
Dynamic Pet ◽  
Arterial Concentration ◽  
In Blood Plasma

Abstract Background In molecular imaging with dynamic PET, the binding and dissociation of a targeted tracer is characterized by kinetics modeling which requires the arterial concentration of the tracer to be measured accurately. Once in the body the radiolabeled parent tracer may be subjected to hydrolysis, demethylation/dealkylation and other biochemical processes, resulting in the production and accumulation of different metabolites in blood which can be labeled with the same PET radionuclide as the parent. Since these radio-metabolites cannot be distinguished by PET scanning from the parent tracer, their contribution to the arterial concentration curve has to be removed for the accurate estimation of kinetic parameters from kinetic analysis of dynamic PET. High-performance liquid chromatography has been used to separate and measure radio-metabolites in blood plasma; however, the method is labor intensive and remains a challenge to implement for each individual patient. The purpose of this study is to develop an alternate technique based on thin layer chromatography (TLC) and a sensitive commercial autoradiography system (Beaver, Ai4R, Nantes, France) to measure radio-metabolites in blood plasma of two targeted tracers—[18F]FAZA and [18F]FEPPA, for imaging hypoxia and inflammation, respectively. Results Radioactivity as low as 17 Bq in 2 µL of pig’s plasma can be detected on the TLC plate using autoradiography. Peaks corresponding to the parent tracer and radio-metabolites could be distinguished in the line profile through each sample (n = 8) in the autoradiographic image. Significant intersubject and intra-subject variability in radio-metabolites production could be observed with both tracers. For [18F]FEPPA, 50% of plasma activity was from radio-metabolites as early as 5-min post injection, while for [18F]FAZA, significant metabolites did not appear until 50-min post. Simulation study investigating the effect of radio-metabolite in the estimation of kinetic parameters indicated that 32–400% parameter error can result without radio-metabolites correction. Conclusion TLC coupled with autoradiography is a good alternative to high-performance liquid chromatography for radio-metabolite correction. The advantages of requiring only small blood samples (~ 100 μL) and of analyzing multiple samples simultaneously, make the method suitable for individual dynamic PET studies.

The metabolism of leukotrienes in blood plasma studied by high-performance liquid chromatography

1985 ◽  
Vol 833 (1) ◽  
pp. 128-134 ◽  
Author(s):  
Manfred Köller ◽  
Wolfgang Schönfeld ◽  
Jürgen Knöller ◽  
Klaus-Dieter Bremm ◽  
Wolfgang König ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Blood Plasma ◽  
High Performance ◽  
In Blood Plasma
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