Duplex RT-PCR and chemiluminometric hybridization assay for combined screening of the mRNAs of prostate-specific antigen and prostate-specific membrane antigen in peripheral blood

2005 ◽  
Vol 531 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Evaggelia Emmanouilidou ◽  
Bakhos Tannous ◽  
Penelope C. Ioannou ◽  
Theodore K. Christopoulos
Keyword(s):  
Prostate Specific Antigen ◽  
Peripheral Blood ◽  
Specific Antigen ◽  
Hybridization Assay ◽  
Prostate Specific Membrane Antigen ◽  
Rt Pcr ◽  
Specific Membrane

scholarly journals Expression of prostate-specific antigen and prostate-specific membrane antigen transcripts in blood cells: implications for the detection of hematogenous prostate cells and standardization

1998 ◽  
Vol 44 (3) ◽  
pp. 472-481 ◽  
Author(s):  
Jean-Luc Gala ◽  
Michel Heusterspreute ◽  
Sylvain Loric ◽  
France Hanon ◽  
Bertrand Tombal ◽  
...  
Keyword(s):  
Cell Line ◽  
Prostate Specific Antigen ◽  
Cell Lines ◽  
Specific Antigen ◽  
Prostate Specific Membrane Antigen ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Blood Samples ◽  
Prostate Cells ◽  
Specific Membrane

Abstract Circulating prostate cells can be detected in cancer patients by using reverse transcriptase–PCR (RT-PCR) assay for prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) mRNA. A quality-control study involving a conventional RT-PCR assay was performed and, surprisingly, detected both transcripts in many negative control cell lines and in normal blood samples. The existence of an illegitimate transcription of the PSA and PSM genes was evidenced by sequence analysis of several PSM and PSA-PCR products. Sequencing indeed demonstrated the presence of a PSA or PSM polymorphism in some but not all the cell lines and patient samples, as well as a heterozygous mutation (G to A; Asp to Asn) in the Jurkat cell line. Moreover, the amount of PSA transcript in MCF-7, a PSA-negative breast line, increased after incubation with cycloheximide. Interestingly, the frequency of positivity was as high as 12% in male samples if only tested once, but dropped to 3% upon multiple testing of the same cDNA. This highlights the stochastic effects in RT-PCR results at high sensitivity, hence the importance of repetitive testing in clinical samples. Decreasing the number of cycles avoided the amplification of illegitimate transcripts but also affected the limit of detection, as evidenced with PSA and PSM cDNA containing plasmids, mixing of LNCap with normal blood samples, and the PSA-PSM-negative K562 cell line. The current data raise the need for a multicentric standardization of the RT-PCR methodology used to amplify PSA and PSM transcripts.

Can 68Ga-PSMA PET/CT-derived prostate-specific membrane antigen expression parameters predict prostate-specific antigen response to enzalutamide treatment?

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Savaş Karyağar ◽  
Osman Güven ◽  
Sevda Sağlampinar Karyağar ◽  
Serdar Arici ◽  
Oğuzhan Selvi ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Expression ◽  
Prostate Specific Membrane Antigen ◽  
Psma Pet ◽  
Pet Ct ◽  
Specific Membrane
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