scholarly journals Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization

2019 ◽  
Vol 667 ◽  
pp. 14-21 ◽  
Author(s):  
Justin S. Plaut ◽  
Agnieszka Strzelecka-Kiliszek ◽  
Lukasz Bozycki ◽  
Slawomir Pikula ◽  
René Buchet ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Matrix Vesicle ◽  
Force Microscopy ◽  
Atomic Force ◽  
Insight Into

Insight into the Deformation Mechanisms under a Sharp Contact Loading in Glass by Atomic Force Microscopy

2005 ◽  
Vol 904 ◽  
Author(s):  
Tanguy Rouxel ◽  
Satoshi Yoshida ◽  
Haixia Shang ◽  
Jean-Christophe Sangleboeuf
Keyword(s):  
Atomic Force Microscopy ◽  
Contact Area ◽  
Elastic Recovery ◽  
Residual Deformation ◽  
Constitutive Law ◽  
Indentation Creep ◽  
Contact Loading ◽  
Force Microscopy ◽  
Atomic Force ◽  
Insight Into

AbstractThe response of a material to a sharp contact loading, as in the case of Vickers indentation for instance, provides a unique insight into the material constitutive law, including elastic and irreversible deformation parameters as well. However, under such peculiar thermodynamical and mechanical conditions (the mean contact pressure on the contact area reaches values typically higher than 1 GPa, corresponding to the hardness of the material) the deformation processes are complex and the matter located just beneath and around the contact area may experience some structural changes and behave in a way different to the expected - or known - macroscopic behaviour. It is showed in this study by means of detailed topological investigations of the residual indentations by Atomic Force Microscopy (AFM) that the elastic recovery typically represents 50 to 70 % of the indentation volume at maximum load and that the densification contribution may reach 90 % of the residual deformation volume. Besides, most glasses exhibit indentation-creep phenomena, which become significant over time scale of few minutes because of a pronounced shear-thinning behavior..

Numerical Analysis of the Band Excitation AFM Method: Examining the Characteristics of the Excitation Signals and the Corresponding Response Behavior at the Cantilever Tip

2011 ◽  
Author(s):  
Adam U. Kareem ◽  
Santiago D. Solares
Keyword(s):  
Atomic Force Microscopy ◽  
Initial Step ◽  
Phase Content ◽  
Frequency Content ◽  
Response Behavior ◽  
Force Microscopy ◽  
Atomic Force ◽  
Sinc Functions ◽  
Insight Into ◽  
Excitation Method

We present numerical simulations of a recently developed atomic force microscopy (AFM) technique known as the Band Excitation Method, developed by Jesse et al. [2007 Nanotechnology 18 435503]. With this technique an AFM microcantilever is simultaneously excited and the response measured over a continuum band of frequencies. The purpose of this work is to introduce an analytical model providing insight into the dynamics of the Band Excitation Method, which can help in the translation of the acquired signals into sample properties. As an initial step we examine the cantilever response to two distinct excitation signals, the chirp and sinc functions, both of which have uniform frequency content, differing only in the phase content.

scholarly journals A Different Protein Corona Cloaks “True-to-Life” Nanoplastics with Respect to Synthetic Polystyrene Nanobeads

2021 ◽  
Author(s):  
Serena Ducoli ◽  
Stefania Federici ◽  
Roland Nicsanu ◽  
Andrea Zendrini ◽  
Claudio Marchesi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Environmental Samples ◽  
Daily Life ◽  
Protein Corona ◽  
Force Microscopy ◽  
Atomic Force ◽  
Mechanical Fragmentation ◽  
Cryogenic Conditions ◽  
Insight Into

Given the complexity of separating nanoplastics from environmental samples, studies have usually been conducted using synthetic polystyrene nanobeads. By mechanical fragmentation in cryogenic conditions of daily-life plastic items, we produced “true-to-life” nanoplastics (T2LNPs), that promises to give a true insight into the interaction with biological systems. T2LNPs have been fully characterized by Fourier transform Infrared spectroscopy and by Atomic Force Microscopy. They result in populations of spheroidal nanoparticles with a broad multimodal size distribution. The mandatory need for a representative sample to evaluate the potential effects of nanoparticles on human health and the environment is demonstrated by the different protein corona identified on T2LNPs and synthetic polystyrene nanobeads upon incubation with human plasma.

scholarly journals A Different Protein Corona Cloaks “True-to-Life” Nanoplastics with Respect to Synthetic Polystyrene Nanobeads

2021 ◽  
Author(s):  
Serena Ducoli ◽  
Stefania Federici ◽  
Roland Nicsanu ◽  
Andrea Zendrini ◽  
Claudio Marchesi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Environmental Samples ◽  
Daily Life ◽  
Protein Corona ◽  
Force Microscopy ◽  
Atomic Force ◽  
Mechanical Fragmentation ◽  
Cryogenic Conditions ◽  
Insight Into

Given the complexity of separating nanoplastics from environmental samples, studies have usually been conducted using synthetic polystyrene nanobeads. By mechanical fragmentation in cryogenic conditions of daily-life plastic items, we produced “true-to-life” nanoplastics (T2LNPs), that promises to give a true insight into the interaction with biological systems. T2LNPs have been fully characterized by Fourier transform Infrared spectroscopy and by Atomic Force Microscopy. They result in populations of spheroidal nanoparticles with a broad multimodal size distribution. The mandatory need for a representative sample to evaluate the potential effects of nanoparticles on human health and the environment is demonstrated by the different protein corona identified on T2LNPs and synthetic polystyrene nanobeads upon incubation with human plasma.

scholarly journals Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5

2017 ◽  
Vol 1859 (10) ◽  
pp. 1911-1920 ◽  
Author(s):  
Maytê Bolean ◽  
Ivana A. Borin ◽  
Ana M.S. Simão ◽  
Massimo Bottini ◽  
Luis A. Bagatolli ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Matrix Vesicle ◽  
Topographic Analysis ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Studying early stages of fibronectin fibrillogenesis in living cells by atomic force microscopy

2015 ◽  
Vol 26 (18) ◽  
pp. 3190-3204 ◽  
Author(s):  
Tetyana Gudzenko ◽  
Clemens M. Franz
Keyword(s):  
Atomic Force Microscopy ◽  
Matrix Protein ◽  
Time Lapse ◽  
Extracellular Matrix Protein ◽  
Force Microscopy ◽  
Extension Mechanism ◽  
Cell Matrix ◽  
Atomic Force ◽  
Complex Linear ◽  
Insight Into

Fibronectin (FN) is an extracellular matrix protein that can be assembled by cells into large fibrillar networks, but the dynamics of FN remodeling and the transition through intermediate fibrillar stages are incompletely understood. Here we used a combination of fluorescence microscopy and time-lapse atomic force microscopy (AFM) to visualize initial stages of FN fibrillogenesis in living fibroblasts at high resolution. Initial FN nanofibrils form within <5 min of cell–matrix contact and subsequently extend at a rate of 0.25 μm/min at sites of cell membrane retraction. FN nanofibrils display a complex linear array of globular features spaced at varying distances, indicating the coexistence of different conformational states within the fibril. In some cases, initial fibrils extended in discrete increments of ∼800 nm during a series of cyclical membrane retractions, indicating a stepwise fibrillar extension mechanism. In presence of Mn2+, a known activator of integrin adhesion to FN, fibrillogenesis was accelerated almost threefold to 0.68 μm/min and fibrillar dimensions were increased, underlining the importance of integrin activation for early FN fibrillogenesis. FN fibrillogenesis visualized by time-lapse AFM thus provides new structural and mechanistic insight into initial steps of cell-driven FN fibrillogenesis.

scholarly journals Analysis of long dsRNA produced in vitro and in vivo using atomic force microscopy in conjunction with ion-pair reverse-phase HPLC

The Analyst ◽  
2019 ◽  
Vol 144 (16) ◽  
pp. 4985-4994
Author(s):  
Alison O. Nwokeoji ◽  
Sandip Kumar ◽  
Peter M. Kilby ◽  
David E. Portwood ◽  
Jamie K. Hobbs ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Performance ◽  
Ion Pair ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Force Microscopy ◽  
Atomic Force ◽  
Insight Into

Atomic force microscopy (AFM) in conjunction with ion-pair reverse-phase high performance liquid chromatography (IP-RP-HPLC) provides novel insight into dsRNA for RNAi applications.

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