scholarly journals Nanopore formation process in artificial cell membrane induced by plasma-generated reactive oxygen species

2016 ◽  
Vol 605 ◽  
pp. 26-33 ◽  
Author(s):  
Ryugo Tero ◽  
Ryuma Yamashita ◽  
Hiroshi Hashizume ◽  
Yoshiyuki Suda ◽  
Hirofumi Takikawa ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Membrane ◽  
Formation Process ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Artificial Cell

scholarly journals Drug-induced reactive oxygen species (ROS) rely on cell membrane properties to exert anticancer effects

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Hamid R. Molavian ◽  
Aaron Goldman ◽  
Colin J. Phipps ◽  
Mohammad Kohandel ◽  
Bradly G. Wouters ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Membrane ◽  
Reactive Oxygen ◽  
Membrane Properties ◽  
Oxygen Species ◽  
Drug Induced ◽  
Anticancer Effects

scholarly journals Comparison of Reactive Oxygen Species Production Activity and Binding Ability of Porphyrins in Cell Membrane Models

2015 ◽  
Vol 108 (2) ◽  
pp. 414a
Author(s):  
Barnabás Böcskei-Antal ◽  
Bianka Nagy ◽  
Szilvia Anikó Tóth ◽  
Nikoletta Kósa ◽  
István Voszka ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Membrane ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Binding Ability ◽  
Production Activity ◽  
Membrane Models ◽  
Species Production

scholarly journals Mechanism of Microbicidal Action of E-101 Solution, a Myeloperoxidase-Mediated Antimicrobial, and its Oxidative Products

2018 ◽  
Author(s):  
G. A. Denys ◽  
Neil C. Devoe ◽  
P. Gudis ◽  
M. May ◽  
R.C. Allen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antimicrobial Activity ◽  
Cell Membrane ◽  
Whole Blood ◽  
Reactive Oxygen ◽  
Cellular Damage ◽  
Oxygen Species ◽  
Protection Assay ◽  
Microbicidal Activity ◽  
Oxidative Products

ABSTRACTE-101 Solution is a first in class myeloperoxidase-mediated antimicrobial developed for topical application. It is composed of porcine myeloperoxidase (pMPO), glucose oxidase (GO), glucose, sodium chloride, and specific amino acids in an aqueous vehicle. Once activated, the reactive species hydrogen peroxide (H2O2), hypochlorous acid and singlet oxygen are generated. We evaluated the treatment effects of E-101 solution and its oxidative products on ultrastucture changes and microbicidal activity against methicillin-resistantStaphylococcus aureus(MRSA) andEscherichia coli. Time kill and transmission electron microscopy studies were performed using formulations with pMPO or GO omitted. The glutathione membrane protection assay was used to study the neutralization of reactive oxygen species. The potency of E-101 solution was also measured in the presence of serum and whole blood by MIC and MBC determinations. E-101 solution demonstrated rapid bactericidal activity and ultracellular changes in MRSA andE. colicells. When pMPO was omitted, high levels of H2O2generated from GO and glucose demonstrated slow microbicidal activity with minimal cellular damage. When GO was omitted from the formulation no antimicrobial activity or cellular damage was observed. Protection from exposure to E-101 solution reactive oxygen species in the glutathione protection assay was competitive and temporary. E-101 solution maintained its antimicrobial activity in the presence of inhibitory substances such as serum and whole blood. E-101 solution is a potent myeloperoxidase enzyme system with multiple oxidative mechanisms of action. Our findings suggest the primary site that E-101solution exerts microbicidal action is the cell membrane by inactivation of essential cell membrane components.

scholarly journals Study of Tissue-Specific Reactive Oxygen Species Formation by Cell Membrane Microarrays for the Characterization of Bioactive Compounds

Membranes ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 943
Author(s):  
Ane Elexpe ◽  
Nerea Nieto ◽  
Claudia Fernández-Cuétara ◽  
Celtia Domínguez-Fernández ◽  
Teresa Morera-Herreras ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Membrane ◽  
Oxidative Damage ◽  
Reactive Oxygen ◽  
Antimalarial Drugs ◽  
Superoxide Production ◽  
Mitochondrial Activity ◽  
Oxygen Species ◽  
Triggering Process

The production of reactive oxygen species (ROS) increases considerably in situations of cellular stress, inducing lipid peroxidation and multiple alterations in proteins and nucleic acids. However, sensitivity to oxidative damage varies between organs and tissues depending on the triggering process. Certain drugs used in the treatment of diverse diseases such as malaria have side effects similar to those produced by oxidative damage, although no specific study has been conducted. For this purpose, cell membrane microarrays were developed and the superoxide production evoked by the mitochondrial activity was assayed in the presence of specific inhibitors: rotenone, antimycin A and azide. Once the protocol was set up on cell membrane isolated from rat brain areas, the effect of six antimalarial drugs (atovaquone, quinidine, doxycycline, mefloquine, artemisinin, and tafenoquine) and two essential oils (Rosmarinus officinalis and Origanum majoricum) were evaluated in multiple human samples. The basal activity was different depending on the type of tissue, the liver, jejunum and adrenal gland being the ones with the highest amount of superoxide. The antimalarial drugs studied showed specific behavior according to the type of human tissue analyzed, with atovaquone and quinidine producing the highest percentage of superoxide formation, and doxycycline the lowest. In conclusion, the analysis of superoxide production evaluated in cell membranes of a collection of human tissues allowed for the characterization of the safety profile of these antimalarial drugs against toxicity mediated by oxidative stress.

Mitochondria as a source of reactive oxygen species

2009 ◽  
pp. c3 ◽  
Author(s):  
Helena M. Cochemé ◽  
Michael P. Murphy
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species

Clinical aspects of reactive oxygen and nitrogen species

2004 ◽  
Vol 71 ◽  
pp. 121-133 ◽  
Author(s):  
Ascan Warnholtz ◽  
Maria Wendt ◽  
Michael August ◽  
Thomas Münzel
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Endothelial Dysfunction ◽  
Vascular Tissue ◽  
Rapid Development ◽  
Reactive Oxygen ◽  
Clinical Aspects ◽  
No Production ◽  
Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

Fas ligand expression in rat kupffer cells in response to lipopolysaccharide is mediated by reactive oxygen species

2001 ◽  
Vol 120 (5) ◽  
pp. A361-A361
Author(s):  
K UCHIKURA ◽  
T WADA ◽  
Z SUN ◽  
S HOSHINO ◽  
G BULKLEY ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Kupffer Cells ◽  
Fas Ligand ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Ligand Expression
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