Differential effect of adenosine receptors on growth of human colon cancer HCT 116 and HT-29 cell lines

2013 ◽  
Vol 533 (1-2) ◽  
pp. 47-54 ◽  
Author(s):  
Monika Sakowicz-Burkiewicz ◽  
Agnieszka Kitowska ◽  
Marzena Grden ◽  
Izabela Maciejewska ◽  
Andrzej Szutowicz ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Adenosine Receptors ◽  
Differential Effect ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Hct 116 ◽  
Ht 29

scholarly journals Celecoxib increases retinoid sensitivity in human colon cancer cell lines

2010 ◽  
Vol 15 (3) ◽  
Author(s):  
Jian-Pei Liu ◽  
Hong-Bo Wei ◽  
Zong-Heng Zheng ◽  
Wei-Ping Guo ◽  
Jia-Feng Fang
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines ◽  
Cox 2 ◽  
Ht 29

AbstractRetinoid resistance has limited the clinical application of retinoids as differentiation-inducing and apoptosis-inducing drugs. This study was designed to investigate whether celecoxib, a selective COX-2 inhibitor, has effects on retinoid sensitivity in human colon cancer cell lines, and to determine the possible mechanism of said effects. Cell viability was measured using the MTT assay. Apoptosis was detected via Annexin-V/PI staining and the flow cytometry assay. PGE2 production was measured with the ELISA assay. The expression of RARβ was assayed via western blotting. The results showed that celecoxib enhanced the inhibitory effect of ATRA in both COX-2 high-expressing HT-29 and COX-2 low-expressing SW480 cell lines. Further study showed the ATRA and celecoxib combination induced greater apoptosis, but that the addition of PGE2 did not affect the enhanced growth-inhibitory and apoptosis-inducing effects of the combination. Moreover, NS398 (another selective COX-2 inhibitor) did not affect the inhibitory effects of ATRA in the two cell lines. Western blotting showed that the expression of RARβ in HT-29 cell lines was increased by celecoxib, but not by NS398, and that the addition of PGE2 did not affect the celecoxib-induced expression of the retinoic acid receptor beta. In conclusion, celecoxib increased the expression of RARβ and the level of cellular ATRA sensitivity through COX-2-independent mechanisms. This finding may provide a potential strategy for combination therapy.

scholarly journals Apoptotic effects of acacetin in human colon cancer HT-29 and HCT 116 cells

2021 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Bedia Cakmakoglu ◽  
BesteTacal Aslan ◽  
Baris Ertugrul ◽  
ElifSinem Iplik
Keyword(s):  
Colon Cancer ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Hct 116 ◽  
Hct 116 Cells ◽  
Apoptotic Effects ◽  
Ht 29

Efficient biogenesis of Au nanoparticles using extract of Mentha pulegium flower: Evaluation of catalytic reduction of nitroarenes and anti-human colon cancer properties in the in vitro condition

2020 ◽  
Author(s):  
Shabnam Bovandi ◽  
Maryam Shahriari ◽  
Mohammad Mahdi Zangeneh
Keyword(s):  
Colon Cancer ◽  
Gold Nanoparticles ◽  
Colorectal Adenocarcinoma ◽  
Human Colon ◽  
Mentha Pulegium ◽  
Human Colon Cancer ◽  
Au Nps ◽  
X Ray ◽  
Hct 116 ◽  
Ht 29

Abstract Background With regards to applicative, facile, green chemical research, a bio-inspired approach is being reported for the synthesis of Au nanoparticles by using Mentha pulegium flower extract. Methods The phytochemical immobilized Au NPs were characterized by advanced physicochemical techniques like Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX), and X-ray Diffraction (XRD) study. Thereafter, catalytic performance of those biomolecule functionalized Au NPs was investigated in the efficient reduction of nitroarenes over a range of substrates. To survey the anti-human colon cancer effects of gold nanoparticles, MTT assay was used on the common colon cancer cell lines i.e., colorectal adenocarcinoma (HT-29), colorectal carcinoma (HCT 116), ileocecal colorectal adenocarcinoma (HCT-8 [HRT-18]), and Burkitt's lymphoma (Ramos.2G6.4C10). Results The conversion was achieved in short reaction time with good to excellent yields in association with outstanding turnover frequency (TOF). In addition, the nanocomposite catalyst was easily recovered and recycled for 12 successive times without noticeable decrease in catalytic activity. Gold nanoparticles had high anti-colon cancer activities dose-dependently against HT-29, HCT 116, HCT-8 [HRT-18], and Ramos.2G6.4C10 cell lines. The best result of anti-colon cancer effects was seen in the case of the HCT 116 cell line. Conclusions It looks gold nanoparticles can be used for the treatment of several types of colon cancers in human.

Sphingoid Bases and Ceramide Induce Apoptosis in HT-29 and HCT-116 Human Colon Cancer Cells

2002 ◽  
Vol 227 (5) ◽  
pp. 345-353 ◽  
Author(s):  
Eun Hyun Ahn ◽  
Joseph J. Schroeder
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Protective Role ◽  
Colon Cancer Cells ◽  
Sphingoid Bases ◽  
Human Colon Cancer ◽  
Hct 116 ◽  
Human Colon Cancer Cells ◽  
Ht 29

Complex dietary sphingolipids such as sphingomyelin and glycosphingolipids have been reported to inhibit development of colon cancer. This protective role may be the result of turnover to bioactive metabolites including sphingoid bases (sphingosine and sphinganine) and ceramide, which inhibit proliferation and stimulate apoptosis. The purpose of the present study was to investigate the effects of sphingoid bases and ceramides on the growth, death, and cell cycle of HT-29 and HCT-116 human colon cancer cells. The importance of the 4,5-trans double bond present in both sphingosine and C2-ceramide (a short chain analog of ceramide) was evaluated by comparing the effects of these lipids with those of sphinganine and C2-dihydroceramide (a short chain analog of dihydroceramide), which lack this structural feature. Sphingosine, sphinganine, and C2-ceramide inhibited growth and caused death of colon cancer cells in time-and concentration-dependent manners, whereas C2-dihydroceramide had no effect. These findings suggest that the 4,5-trans double bond is necessary for the inhibitory effects of C2-ceramide, but not for sphingoid bases. Evaluation of cellular morphology via fluorescence microscopy and quantitation of fragmented low-molecular weight DNA using the diphenylamine assay demonstrated that sphingoid bases and C2-ceramide cause chromatin and nuclear condensation as well as fragmentation of DNA, suggesting these lipids kill colon cancer cells by Inducing apoptosis. Flow cytometric analyses confirmed that sphingoid bases and C2-ceramide increased the number of cells in the A0 peak indicative of apoptosis and demonstrated that sphingoid bases arrest the cell cycle at G2/M phase and cause accumulation in the S phase. These findings establish that sphingoid bases and ceramide induce apoptosis In colon cancer cells and implicate them as potential mediators of the protective role of more complex dietary sphingolipids in colon carcinogenesis.

scholarly journals Synthesis and cellular effects of novel 1,3,5-triazine derivatives in DLD and Ht-29 human colon cancer cell lines

2019 ◽  
Vol 38 (4) ◽  
pp. 990-1002 ◽  
Author(s):  
Agnieszka Wróbel ◽  
Beata Kolesińska ◽  
Justyna Frączyk ◽  
Zbigniew J. Kamiński ◽  
Anna Tankiewicz-Kwedlo ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Cellular Effects ◽  
Colon Cancer Cell Lines ◽  
Ht 29

Summary This study provides new information on the cellular effects of 1,3,5-triazine nitrogen mustards with different peptide groups in DLD and Ht-29 human colon cancer cell lines. A novel series of 2,4,6-trisubstituted 1,3,5-triazine derivatives bearing 2-chloroethyl and oligopeptide moieties was designed and synthesized. The most cytotoxic derivative was triazine with an Ala-Ala-OMe substituent on the ring (compound 7b). This compound induced time- and dose-dependent cytotoxicity in the DLD-1 and HT-29 colon cancer cell lines. The triazine derivative furthermore induced apoptosis through intracellular signaling pathway attenuation. Compound 7b may be a candidate for further evaluation as a chemotherapeutic agent against colorectal cancer.

scholarly journals Cytotoxicity of aporphines in human colon cancer cell lines HCT-116 and Caco-2: An SAR study

2011 ◽  
Vol 21 (15) ◽  
pp. 4462-4464 ◽  
Author(s):  
Shashikanth Ponnala ◽  
Sandeep Chaudhary ◽  
Antonio González-Sarrias ◽  
Navindra P. Seeram ◽  
Wayne W. Harding
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines ◽  
Hct 116 ◽  
Sar Study
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