The influence of active site loop mutations on the thermal stability of azurin from Pseudomonas aeruginosa

2012 ◽  
Vol 521 (1-2) ◽  
pp. 18-23 ◽  
Author(s):  
Rita Guzzi ◽  
Luigi Sportelli ◽  
Sachiko Yanagisawa ◽  
Chan Li ◽  
Dorota Kostrz ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Pseudomonas Aeruginosa ◽  
Active Site ◽  
Thermal Stability Of

scholarly journals Contribution of active site residues to the activity and thermal stability of ribonuclease Sa

2009 ◽  
Vol 12 (10) ◽  
pp. 2367-2373 ◽  
Author(s):  
Gennady I. Yakovlev ◽  
Vladimir A. Mitkevich ◽  
Kevin L. Shaw ◽  
Saul Trevino ◽  
Stephanie Newsom ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Active Site ◽  
Active Site Residues ◽  
Ribonuclease Sa ◽  
Thermal Stability Of

scholarly journals Antibacterial effects assessment on some livestock pathogens, thermal stability and proposing a probable reason for different levels of activity of thanatin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ali Javadmanesh ◽  
Elyas Mohammadi ◽  
Zahra Mousavi ◽  
Marjan Azghandi ◽  
Abass Tanhaiean
Keyword(s):  
Escherichia Coli ◽  
Thermal Stability ◽  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Farm Animals ◽  
Probable Reason ◽  
Thermal Stability Of

AbstractThere is a continuing need to prevent the increasing use of common antibiotic and find the replacement to combat the drug/antibiotic resistant bacteria such as antimicrobial peptides (AMPs) such as thanatin peptide. In this study, recombinant thanatin peptide was expressed in the HEK293 cell line. Then the antimicrobial properties of this peptide on some poultry and farm animal’s pathogen strains were assessed. The thermal-stability of thanatin was predicted in various temperatures through in silico analysis. Afterwards, according to Minimum Inhibitory Concentration (MIC) results, Escherichia coli and Pseudomonas aeruginosa were chosen to test the hypothesis of LptA/LptD–thanatin interaction, computationally. Relative amino acid sequences and crystallography structures were retrieved and missed tertiary structures were predicted. The interaction of thanatin with LptA and LptD of Escherichia coli and Pseudomonas aeruginosa were analyzed subsequently. The antibacterial activity of thanatin peptide was evaluated between 6.25 and 100 μg/mL using minimum inhibitory concentration. Also, the amounts of minimum bactericidal concentrations (MBC) were between 12.5 and 200 μg/mL. The bioinformatics analysis followed by the in vitro assessment, demonstrated that thanatin would be thermally stable in the body temperature of poultry and farm animals. Thanatin could penetrate to the outer membrane domain of LptD in Escherichia coli and it could block the transition path of this protein while the entrance of LptD in Pseudomonas aeruginosa was blocked for thanatin by extra residues in comparison with Escherichia coli LptD. In addition, the quality of interaction, with regard to the number and distance of interactions which leads to higher binding energy for thanatin and LptD of Escherichia coli was much better than Pseudomonas aeruginosa. But the site and quality of interaction for thanatin and LptA was almost the same for Escherichia coli and Pseudomonas aeruginosa. Accordingly, thanatin can prevent the assembly of LptA periplasmic bridge in both pathogens. The antibacterial and thermal stability of the thanatin peptide suggested that thanatin peptide might serve as a natural alternative instead of common antibiotics in the veterinary medicine. The outcome of this in silico study supports the MIC results. Therefore, a probable reason for different level of activity of thanatin against Escherichia coli and Pseudomonas aeruginosa might be the quality of LptA/LptD–thanatin interaction.

Direct Decomposition of NO into N2 and O2 over Cu /ZSM-5 Containing Ce and Zr as Promoter

2010 ◽  
Vol 113-116 ◽  
pp. 1735-1739 ◽  
Author(s):  
Peng Fei Yang ◽  
Ji Cheng Zhou ◽  
Zhe Wang
Keyword(s):  
Thermal Stability ◽  
Ion Exchange ◽  
High Activity ◽  
Active Site ◽  
Direct Decomposition ◽  
No Decomposition ◽  
Highly Active ◽  
Zr Addition ◽  
Decomposition Of No ◽  
Thermal Stability Of

The Zr-Cu-Ce/ZSM-5 catalysts prepared by the simultaneous ion-exchange showed high activity for NO decomposition in the presence of O2, the highest conversion of NO was up to 75%, indicating that the Ce and Zr addition can enhance the activity of catalysts. A new highly active site, which facilitates oxygen mobilization and desorption, could be formed in the sample due to the Ce and Zr addition. In addition, the Zr addition can significantly improve the thermal stability of the catalyst.

Enhanced thermal stability of Pseudomonas aeruginosa lipoxygenase through modification of two highly flexible regions

2013 ◽  
Vol 98 (4) ◽  
pp. 1663-1669 ◽  
Author(s):  
Xinyao Lu ◽  
Song Liu ◽  
Yue Feng ◽  
Shengqi Rao ◽  
Xiaoman Zhou ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Pseudomonas Aeruginosa ◽  
Thermal Stability Of

The Ordered Phase Formation in Ti-Al-Ga Alloys

1970 ◽  
Vol 28 ◽  
pp. 440-441
Author(s):  
Shiro Fujishiro ◽  
Harold L. Gegel
Keyword(s):  
Thermal Stability ◽  
High Temperature ◽  
Titanium Alloys ◽  
Growth Kinetics ◽  
Stoichiometric Composition ◽  
Good Potential ◽  
Alpha Titanium ◽  
Cold Rolled ◽  
Kinetics Of ◽  
Thermal Stability Of

Ordered-alpha titanium alloys having a DO19 type structure have good potential for high temperature (600°C) applications, due to the thermal stability of the ordered phase and the inherent resistance to recrystallization of these alloys. Five different Ti-Al-Ga alloys consisting of equal atomic percents of aluminum and gallium solute additions up to the stoichiometric composition, Ti3(Al, Ga), were used to study the growth kinetics of the ordered phase and the nature of its interface.The alloys were homogenized in the beta region in a vacuum of about 5×10-7 torr, furnace cooled; reheated in air to 50°C below the alpha transus for hot working. The alloys were subsequently acid cleaned, annealed in vacuo, and cold rolled to about. 050 inch prior to additional homogenization

Microstructure studies of tungsten silicide schottky contacts on Gaas

1987 ◽  
Vol 45 ◽  
pp. 328-329
Author(s):  
Yih-Cheng Shih ◽  
E. L. Wilkie
Keyword(s):  
Thermal Stability ◽  
Field Effect Transistors ◽  
Schottky Contact ◽  
Schottky Contacts ◽  
Excellent Thermal Stability ◽  
Barrier Heights ◽  
Gaas Substrates ◽  
Film Adhesion ◽  
Threshold Voltages ◽  
Thermal Stability Of

Tungsten silicides (WSix) have been successfully used as the gate materials in self-aligned GaAs metal-semiconductor-field- effect transistors (MESFET). Thermal stability of the WSix/GaAs Schottky contact is of major concern since the n+ implanted source/drain regions must be annealed at high temperatures (∼ 800°C). WSi0.6 was considered the best composition to achieve good device performance due to its low stress and excellent thermal stability of the WSix/GaAs interface. The film adhesion and the uniformity in barrier heights and ideality factors of the WSi0.6 films have been improved by depositing a thin layer of pure W as the first layer on GaAs prior to WSi0.6 deposition. Recently WSi0.1 has been used successfully as the gate material in 1x10 μm GaAs FET's on the GaAs substrates which were sputter-cleaned prior to deposition. These GaAs FET's exhibited uniform threshold voltages across a 51 mm wafer with good film adhesion after annealing at 800°C for 10 min.

Thermal stability of Al-Cu-Fe icosahedral alloys

1991 ◽  
Vol 1 (12) ◽  
pp. 1823-1836 ◽  
Author(s):  
M. Bessière ◽  
A. Quivy ◽  
S. Lefebvre ◽  
J. Devaud-Rzepski ◽  
Y. Calvayrac
Keyword(s):  
Thermal Stability ◽  
Thermal Stability Of

Practical criteria for the thermal stability of a unidimensional superconductor

1994 ◽  
Vol 4 (4) ◽  
pp. 653-657
Author(s):  
B. Bonzi ◽  
M. El Khomssi ◽  
H. Lanchon-Ducauquis
Keyword(s):  
Thermal Stability ◽  
Thermal Stability Of

Time and thermal stability of magnetic properties of amorphous Fe80TM3B17 alloys

1998 ◽  
Vol 08 (PR2) ◽  
pp. Pr2-63-Pr2-66 ◽  
Author(s):  
R. Varga ◽  
P. Vojtaník ◽  
A. Lovas
Keyword(s):  
Thermal Stability ◽  
Magnetic Properties ◽  
Thermal Stability Of
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