Urinary excretion of 8-oxo-7,8-dihydroguanine as biomarker of oxidative damage to DNA

2012 ◽  
Vol 518 (2) ◽  
pp. 142-150 ◽  
Author(s):  
Steffen Loft ◽  
Pernille Danielsen ◽  
Mille Løhr ◽  
Kim Jantzen ◽  
Jette G. Hemmingsen ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Urinary Excretion ◽  
Oxidative Damage To Dna

Biomarkers of oxidative damage to DNA and repair

2008 ◽  
Vol 36 (5) ◽  
pp. 1071-1076 ◽  
Author(s):  
Steffen Loft ◽  
Pernille Høgh Danielsen ◽  
Lone Mikkelsen ◽  
Lotte Risom ◽  
Lykke Forchhammer ◽  
...  
Keyword(s):  
Comet Assay ◽  
Oxidative Damage ◽  
Urinary Excretion ◽  
Oxidative Dna Damage ◽  
Excision Repair ◽  
Population Based ◽  
Base Excision ◽  
Cellular Dna ◽  
Risk Of Cancer ◽  
Oxidative Damage To Dna

Oxidative-stress-induced damage to DNA includes a multitude of lesions, many of which are mutagenic and have multiple roles in cancer and aging. Many lesions have been characterized by MS-based methods after extraction and digestion of DNA. These preparation steps may cause spurious base oxidation, which is less likely to occur with methods such as the comet assay, which are based on nicking of the DNA strand at modified bases, but offer less specificity. The European Standards Committee on Oxidative DNA Damage has concluded that the true levels of the most widely studied lesion, 8-oxodG (8-oxo-7,8-dihydro-2′-deoxyguanosine), in cellular DNA is between 0.5 and 5 lesions per 106 dG bases. Base excision repair of oxidative damage to DNA can be assessed by nicking assays based on oligonucleotides with lesions or the comet assay, by mRNA expression levels or, in the case of, e.g., OGG1 (8-oxoguanine DNA glycosylase 1), responsible for repair of 8-oxodG, by genotyping. Products of repair in DNA or the nucleotide pool, such as 8-oxodG, excreted into the urine can be assessed by MS-based methods and generally reflects the rate of damage. Experimental and population-based studies indicate that many environmental factors, including particulate air pollution, cause oxidative damage to DNA, whereas diets rich in fruit and vegetables or antioxidant supplements may reduce the levels and enhance repair. Urinary excretion of 8-oxodG, genotype and expression of OGG1 have been associated with risk of cancer in cohort settings, whereas altered levels of damage, repair or urinary excretion in case-control settings may be a consequence rather than the cause of the disease.

scholarly journals Oxidative damage to DNA: Inhibition of guanine damage

2006 ◽  
Vol 78 (12) ◽  
pp. 2297-2304 ◽  
Author(s):  
Sriram Kanvah ◽  
Gary B. Schuster
Keyword(s):  
Oxidative Damage ◽  
Structural Changes ◽  
Global Structure ◽  
Chemical Damage ◽  
Oxidation Of Dna ◽  
Anthraquinone Derivatives ◽  
Covalently Linked ◽  
The One ◽  
Oxidative Damage To Dna ◽  
Dna Inhibition

One-electron oxidation of DNA results in chemical damage to nucleobases, particularly guanine in multiple G sequences. Oxidation may be triggered by numerous events, including photosensitization. We describe studies of photoinduced oxidations of DNA triggered by irradiation of covalently linked anthraquinone derivatives under various conditions that affect the global structure of the DNA. These structural changes have subtle effects on the result of the one-electron oxidation.

scholarly journals Oxidative damage to DNA: do we have a reliable biomarker?

1996 ◽  
Vol 104 (suppl 3) ◽  
pp. 465-469 ◽  
Author(s):  
A R Collins ◽  
M Dusinská ◽  
C M Gedik ◽  
R Stĕtina
Keyword(s):  
Oxidative Damage ◽  
Oxidative Damage To Dna

Does measurement of oxidative damage to DNA have clinical significance?

2006 ◽  
Vol 365 (1-2) ◽  
pp. 30-49 ◽  
Author(s):  
Marcus S. Cooke ◽  
Ryszard Olinski ◽  
Mark D. Evans
Keyword(s):  
Oxidative Damage ◽  
Clinical Significance ◽  
Oxidative Damage To Dna

Simvastatin treatment prevents oxidative damage to DNA in whole blood leukocytes of dyslipidemic type 2 diabetic patients

2010 ◽  
Vol 28 (5) ◽  
pp. 360-366 ◽  
Author(s):  
Vanusa Manfredini ◽  
Giovana Brondani Biancini ◽  
Camila Simioni Vanzin ◽  
Anna Maria Ribeiro Dal Vesco ◽  
Franciele Cipriani ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Whole Blood ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Blood Leukocytes ◽  
Simvastatin Treatment ◽  
Oxidative Damage To Dna ◽  
Type 2 Diabetic
Sign in / Sign up

Export Citation Format

Share Document