Biochemical characterization of l-DOPA 2,3-dioxygenase, a single-domain type I extradiol dioxygenase from lincomycin biosynthesis

2008 ◽  
Vol 479 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Keri L. Colabroy ◽  
William T. Hackett ◽  
Andrew J. Markham ◽  
Jennifer Rosenberg ◽  
David E. Cohen ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Single Domain ◽  
Type I ◽  
Extradiol Dioxygenase ◽  
Domain Type

scholarly journals Both modular and single-domain Type I polyketide synthases are expressed in the brevetoxin-producing dinoflagellate, Karenia brevis (Dinophyceae)

2017 ◽  
Vol 53 (6) ◽  
pp. 1325-1339 ◽  
Author(s):  
Frances M. Van Dolah ◽  
Gurjeet S. Kohli ◽  
Jeanine S. Morey ◽  
Shauna A. Murray
Keyword(s):  
Karenia Brevis ◽  
Polyketide Synthases ◽  
Single Domain ◽  
Type I ◽  
Domain Type

Expression and biochemical characterization of a novel type I pullulanase from Bacillus megaterium

2016 ◽  
Vol 39 (3) ◽  
pp. 397-405 ◽  
Author(s):  
Shaoqing Yang ◽  
Qiaojuan Yan ◽  
Qingdan Bao ◽  
Jingjing Liu ◽  
Zhengqiang Jiang
Keyword(s):  
Bacillus Megaterium ◽  
Biochemical Characterization ◽  
Type I

scholarly journals Purification and biochemical characterization of pullulanase type I fromThermus caldophilusGK-24

1996 ◽  
Vol 138 (2-3) ◽  
pp. 147-152 ◽  
Author(s):  
Cheorl-Ho Kim ◽  
Oyekanmi Nashiru ◽  
Jeong Heon Ko
Keyword(s):  
Biochemical Characterization ◽  
Type I

Molecular cloning and biochemical characterization of a heat-stable type I pullulanase from Thermotoga neapolitana

2011 ◽  
Vol 48 (3) ◽  
pp. 260-266 ◽  
Author(s):  
Jinho Kang ◽  
Kyung-Min Park ◽  
Kyoung-Hwa Choi ◽  
Cheon-Seok Park ◽  
Go-Eun Kim ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Biochemical Characterization ◽  
Thermotoga Neapolitana ◽  
Type I ◽  
Stable Type ◽  
Heat Stable

Biochemical Characterization of a Novel Thermostable Type I Pullulanase Produced Recombinantly inBacillus subtilis

2018 ◽  
Vol 70 (5-6) ◽  
pp. 1700179 ◽  
Author(s):  
Lingmeng Li ◽  
Fengying Dong ◽  
Lin Lin ◽  
Dannong He ◽  
Jingwen Chen ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Type I

scholarly journals Overview of the most commonly used methods in allergen characterization

2005 ◽  
Vol 70 (3) ◽  
pp. 347-360 ◽  
Author(s):  
Tanja Cirkovic-Velickovic ◽  
Marija Gavrovic-Jankulovic ◽  
Ratko Jankov
Keyword(s):  
Biochemical Characterization ◽  
Protein Structures ◽  
Protein Molecule ◽  
Underlying Disease ◽  
Type I ◽  
Molecular Weight Determination ◽  
Aminoacid Sequence ◽  
Allergen Molecule

The characterization of an allergen is a troublesome and difficult process, as it requires both the precise biochemical characterization of a (glyco)protein molecule and the establishment of its susceptibility to IgE antibodies, as they are the main link to histamine release in some hypersensitivity states (type I allergies). As the characterization of an allergen includes molecular weight determination of the allergenic molecule, its structure determination, physicochemical properties, IgE binding properties of the allergen molecule, and its allergenicity, an overall review of which biochemical and immunochemical methods are used in achieving this goal are presented in this paper. The information on the molecular level on the structures of allergens indicates that allergens are considerably heterogeneous protein structures, and that there is no particular aminoacid sequence which is responsible for the allergenicity. Therefore, information gained from detailed structural, functional and immunochemical studies of these intriguing molecules, which nowadays modulate a variety of pathophysiological conditions, would greatly improve our understanding of the underlying disease mechanisms, and the way to handle them.

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