Stimulation of the DNA unwinding activity of human DNA helicase II/Ku by phosphorylation

2008 ◽  
Vol 470 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Alexander E. Ochem ◽  
Hocine Rechreche ◽  
Doris Skopac ◽  
Arturo Falaschi
Keyword(s):  
Dna Helicase ◽  
Dna Unwinding ◽  
Dna Helicase Ii ◽  
Human Dna ◽  
Stimulation Of

scholarly journals Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen.

1994 ◽  
Vol 13 (20) ◽  
pp. 4991-5001 ◽  
Author(s):  
N. Tuteja ◽  
R. Tuteja ◽  
A. Ochem ◽  
P. Taneja ◽  
N.W. Huang ◽  
...  
Keyword(s):  
Dna Helicase ◽  
Dna Unwinding ◽  
Dna Helicase Ii ◽  
Human Dna ◽  
Ku Autoantigen

Inhibition of DNA Unwinding and ATPase Activities of Human DNA Helicase II by Chemotherapeutic Agents

1997 ◽  
Vol 236 (3) ◽  
pp. 636-640 ◽  
Author(s):  
Narendra Tuteja ◽  
Tuan-Nghia Phan ◽  
Renu Tuteja ◽  
Alexander Ochem ◽  
Arturo Falaschi
Keyword(s):  
Dna Helicase ◽  
Chemotherapeutic Agents ◽  
Dna Unwinding ◽  
Dna Helicase Ii ◽  
Human Dna

scholarly journals Stimulation of the UvrABC enzyme-catalyzed repair reactions by the UvrD protein (DNA helicase II)

1985 ◽  
Vol 13 (5) ◽  
pp. 1483-1492 ◽  
Author(s):  
Keiko Kumura ◽  
Mutsuo Sekiguchi ◽  
Anne-Lill Stemum ◽  
Erling Seeberg
Keyword(s):  
Dna Helicase ◽  
Dna Helicase Ii ◽  
Stimulation Of

scholarly journals Synthetic DNA Replication Bubbles Bound and Unwound with Twofold Symmetry by a Simian Virus 40 T-Antigen Double Hexamer

1998 ◽  
Vol 72 (11) ◽  
pp. 8676-8681 ◽  
Author(s):  
Natalia V. Smelkova ◽  
James A. Borowiec
Keyword(s):  
Dna Replication ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Dna Helicase ◽  
T Antigen ◽  
Dna Unwinding ◽  
Replication Forks ◽  
Helicase Activity ◽  
Synthetic Dna ◽  
Stimulation Of

ABSTRACT Dimerization of simian virus 40 T-antigen hexamers (TAgH) into double hexamers (TAgDH) on model DNA replication forks has been found to greatly stimulate T-antigen DNA helicase activity. To explore the interaction of TAgDH with DNA during unwinding, we examined the binding of TAgDH to synthetic DNA replication bubbles. Tests of replication bubble substrates containing different single-stranded DNA (ssDNA) lengths indicated that efficient formation of a TAgDH requires ≥40 nucleotides (nt) of ssDNA. DNase I probing of a substrate containing a 60-nt ssDNA bubble complexed with a TAgDH revealed that T antigen bound the substrate with twofold symmetry. The strongest protection was observed over the 5′ junction on each strand, with 5 bp of duplex DNA and ∼17 nt of adjacent ssDNA protected from nuclease cleavage. Stimulation of the T-antigen DNA helicase activity by an increase in ATP concentration caused the protection to extend in the 5′ direction into the duplex region, while resulting in no significant changes to the 3′ edge of strongest protection. Our data indicate that each TAgH encircles one ssDNA strand, with a different strand bound at each junction. The process of DNA unwinding results in each TAgH interacting with a greater length of DNA than was initially bound, suggesting the generation of a more highly processive helicase complex.

scholarly journals Functional Properties of the Separate Subunits of Human DNA Helicase II/Ku Autoantigen

1997 ◽  
Vol 272 (47) ◽  
pp. 29919-29926 ◽  
Author(s):  
Alexander E. Ochem ◽  
Doris Skopac ◽  
Mario Costa ◽  
Thierry Rabilloud ◽  
Laurent Vuillard ◽  
...  
Keyword(s):  
Functional Properties ◽  
Dna Helicase ◽  
Dna Helicase Ii ◽  
Human Dna ◽  
Ku Autoantigen

A tick homologue of the human DNA helicase II 70-kDa subunit

1996 ◽  
Vol 1305 (3) ◽  
pp. 120-124 ◽  
Author(s):  
Guido C. Paesen ◽  
Paolo M. de A. Zanotto ◽  
Patricia A. Nuttall
Keyword(s):  
Dna Helicase ◽  
Dna Helicase Ii ◽  
Human Dna

scholarly journals A Monoclonal Antibody Raised Against an 86-kD Subunit of Human DNA Helicase II

2000 ◽  
Vol 17 (9) ◽  
pp. 1255-1259
Author(s):  
Maki Murata-Hori ◽  
Takashi Uchimura ◽  
Natsumi Hosoya ◽  
Takahiro Iwasaki ◽  
Saburo Omata ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dna Helicase ◽  
Dna Helicase Ii ◽  
Human Dna

scholarly journals Human DNA helicase V, a novel DNA unwinding enzyme from HeLa cells

1993 ◽  
Vol 21 (10) ◽  
pp. 2323-2329 ◽  
Author(s):  
Narendra Tuteja ◽  
Khalilur Rahman ◽  
Renu Tuteja ◽  
Arturo Falaschi
Keyword(s):  
Hela Cells ◽  
Dna Helicase ◽  
Dna Unwinding ◽  
Human Dna
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