Mutational analysis of the nucleotide binding site of Escherichia coli dCTP deaminase

2008 ◽  
Vol 470 (1) ◽  
pp. 20-26 ◽  
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Majbritt Thymark ◽  
Eva Johansson ◽  
Sine Larsen ◽  
Martin Willemoës
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...  

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William D. Donachie

ABSTRACT Resolution of chromosome dimers, by site-specific recombination between dif sites, is carried out in Escherichia coli by XerCD recombinase in association with the FtsK protein. We show here that a variety of altered FtsK polypeptides, consisting of the N-terminal (cell division) domain alone or with deletions in the proline-glutamine-rich part of the protein, or polypeptides consisting of the C-terminal domain alone are all unable to carry outdif recombination. Alteration of the putative nucleotide-binding site also abolishes the ability of FtsK to carry out recombination between dif sites.


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