Differential changes in phospholipase D and phosphatidate phosphohydrolase activities in ischemia–reperfusion of rat heart

2005 ◽  
Vol 436 (1) ◽  
pp. 136-144 ◽  
Author(s):  
Girma Asemu ◽  
Melissa R. Dent ◽  
Tushi Singal ◽  
Naranjan S. Dhalla ◽  
Paramjit S. Tappia
Keyword(s):  
Phospholipase D ◽  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Phosphatidate Phosphohydrolase

Effect of Hydrogen Sulfide on Reactions of Isolated Rat Heart under Volume Load and Ischemia-Reperfusion

2013 ◽  
Vol 4 (3) ◽  
pp. 201-212
Author(s):  
Tetyana V Shimanskaya ◽  
Yulia V. Goshovska ◽  
Olena M. Semenykhina ◽  
Vadim F. Sagach
Keyword(s):  
Hydrogen Sulfide ◽  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Volume Load ◽  
Isolated Rat

Abstract 104: Effects of Pinacidil and Ca 2 + on Sodium-loaded Rat Heart Mitochondria

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Sergey M Korotkov ◽  
Vladimir P Nesterov ◽  
Irina V Brailovskaya ◽  
Larisa V Emelyanova ◽  
Svetlana A Konovalova ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Rat Heart ◽  
Mitochondrial Membrane ◽  
Mitochondrial Permeability Transition ◽  
Ischemia Reperfusion ◽  
Potassium Acetate ◽  
Heart Mitochondria ◽  
Inner Mitochondrial Membrane ◽  
Rat Heart Mitochondria ◽  
Acetate Medium

Deterioration of the contractile parameters of the heart muscle caused by ischemia and followed reperfusion is known as the main postoperative complication which is related to Ca 2+ and Na + overload in cardiomyocytes and mitochondria. Pinacidil reduced the overload in ischemia/reperfusion experiments. The mechanism of this phenomenon is still not clear. We hypothesized that increased ion permeability of the inner mitochondrial membrane (IMM) followed drop of electrochemical potential (ΔΨ mito ) can reduce the calcium. The aim of the study was to elucidate the effect of pinacidil (100 μM) and Ca 2+ (100 μM ) on swelling, oxygen consumption and ΔΨ mito of isolated sodium-loaded rat heart mitochondria (RHM(Na)) energized glutamate and malate. Pinacidil significantly enchanced the permeability of IMM to protons in ammonium nitrate medium. Also increased swelling of RHM(Na) energized with substrates in potassium acetate medium revealed that pinacidil increased potassium transport into matrix. Pinacidil stimulated oxygen consumption of RHM(Na) in State 4 and detained Ca 2+ -induced dissipation of ΔΨ mito . Under condition of Ca 2+ and Na + overload simulating ischemia/reperfusion, RHM(Na) oxygen consumption was not affected with pinacidil in State 3 and in the presence of 2,4-dinitrophenol. Cyclosporin A and ADP, the inhibitors of mitochondrial permeability transition pore (MPTP), markedly decreased Ca 2+ - induced swelling of RHM(Na) in nitrate ammonium or potassium acetate medium in the presence of pinacidil. Carboxyatractyloside, an inhibitor of cytosolic side-specific adenine nucleotide translocase, eliminated a pinacidil-stimulated oxygen consumption of succinate-energized RHMNa in State 4 regardless of the presence of Ca 2+ . Pinacidil was also concluded to accelerat potassium flux into energized RHM(Na) and promot MPTP opening in the low conduction state. Based on our data we suggested that the effect of pharmacological preconditioning induced by pinacidil could be due to it’s direct effect on mitochondria which is connected with above stimulation of the potassium permeability of the inner mitochondrial membrane and following reduce of the ΔΨ mito that thus prevent calcium overload of cardiomyocytes after ischemia/reperfusion in turn.

Networking Antioxidants in the Isolated Rat Heart are Selectively Depleted by Ischemia-Reperfusion

1998 ◽  
Vol 25 (3) ◽  
pp. 329-339 ◽  
Author(s):  
Nobuya Haramaki ◽  
Daphne B Stewart ◽  
Sarita Aggarwal ◽  
Hisao Ikeda ◽  
Abraham Z Reznick ◽  
...  
Keyword(s):  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Isolated Rat

Influence of antithrombin on ischemia/reperfusion injury in the isolated blood-free perfused rat heart

2002 ◽  
Vol 108 (4) ◽  
pp. 249-255 ◽  
Author(s):  
Josef Margreiter ◽  
Markus Mittermayr ◽  
Johannes Mair ◽  
Angelika Hammerer-Lercher ◽  
Jordan Kountchev ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Rat Heart ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Perfused Rat Heart

Ischemia–reperfusion alters gene expression of Na+–K+ ATPase isoforms in rat heart

2003 ◽  
Vol 306 (2) ◽  
pp. 457-462 ◽  
Author(s):  
Petr Ostadal ◽  
Adel B Elmoselhi ◽  
Irena Zdobnicka ◽  
Anton Lukas ◽  
Donald Chapman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Heart ◽  
Ischemia Reperfusion
Sign in / Sign up

Export Citation Format

Share Document