Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands

Mirela Cristea; Åke Engström; Chao Su; Lena Hörnsten; Ernst H. Oliw

doi:10.1016/j.abb.2004.10.026

Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2004.10.026 ◽

2005 ◽

Vol 434 (1) ◽

pp. 201-211 ◽

Cited By ~ 37

Author(s):

Mirela Cristea ◽

Åke Engström ◽

Chao Su ◽

Lena Hörnsten ◽

Ernst H. Oliw

Keyword(s):

Pichia Pastoris ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Metal Ligands

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Site-directed mutagenesis of an Aspergillus niger xylanase B and its expression, purification and enzymatic characterization in Pichia pastoris

Process Biochemistry ◽

10.1016/j.procbio.2009.08.009 ◽

2010 ◽

Vol 45 (1) ◽

pp. 75-80 ◽

Cited By ~ 17

Author(s):

Xingzhou Chen ◽

Shunqing Xu ◽

Maosheng Zhu ◽

Luosheng Cui ◽

Hui Zhu ◽

...

Keyword(s):

Aspergillus Niger ◽

Pichia Pastoris ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Enzymatic Characterization

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Expression of the Zn2+-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris— utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis

Plant Science ◽

10.1016/s0168-9452(98)00173-3 ◽

1998 ◽

Vol 139 (1) ◽

pp. 19-27 ◽

Cited By ~ 25

Author(s):

Klaus Trummler ◽

Jürgen Roos ◽

Ulrich Schwaneberg ◽

Franz Effenberger ◽

Siegfried Förster ◽

...

Keyword(s):

Pichia Pastoris ◽

Linum Usitatissimum ◽

Recombinant Enzyme ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Enzymatic Analysis ◽

Hydroxynitrile Lyase

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Anti-oxidative effect of ribonuclease inhibitor by site-directed mutagenesis and expression in Pichia pastoris

Frontiers of Biology in China ◽

10.1007/s11515-006-0023-x ◽

2006 ◽

Vol 1 (2) ◽

pp. 99-103

Author(s):

Yu Wu ◽

Xiuyun Cui ◽

Jihong Wang ◽

Peng Zhao ◽

Yuefei Xu ◽

...

Keyword(s):

Pichia Pastoris ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Ribonuclease Inhibitor ◽

Oxidative Effect

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Site-directed mutagenesis of disulfide bridges in Aspergillus niger NRRL 3135 phytase (PhyA), their expression in Pichia pastoris and catalytic characterization

Applied Microbiology and Biotechnology ◽

10.1007/s00253-010-2542-2 ◽

2010 ◽

Vol 87 (4) ◽

pp. 1367-1372 ◽

Cited By ~ 11

Author(s):

Edward J. Mullaney ◽

Heather Locovare ◽

Kandan Sethumadhavan ◽

Stephanie Boone ◽

Xin Gen Lei ◽

...

Keyword(s):

Aspergillus Niger ◽

Pichia Pastoris ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Disulfide Bridges ◽

Catalytic Characterization

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Site-Directed Mutagenesis Improves Catalytic Efficiency and Thermostability of Escherichia coli pH 2.5 Acid Phosphatase/Phytase Expressed in Pichia pastoris

Archives of Biochemistry and Biophysics ◽

10.1006/abbi.2000.2021 ◽

2000 ◽

Vol 382 (1) ◽

pp. 105-112 ◽

Cited By ~ 79

Author(s):

Eric Rodriguez ◽

Zachary A Wood ◽

P.Andrew Karplus ◽

Xin Gen Lei

Keyword(s):

Escherichia Coli ◽

Acid Phosphatase ◽

Pichia Pastoris ◽

Catalytic Efficiency ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Cloning of the nitrile hydratase gene from Nocardia sp. in Escherichia coli and Pichia pastoris and its functional expression using site-directed mutagenesis

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2004.08.007 ◽

2004 ◽

Vol 35 (6-7) ◽

pp. 557-562 ◽

Cited By ~ 19

Author(s):

Yue Shi ◽

Huimin Yu ◽

Xudong Sun ◽

Zhuoling Tian ◽

Zhongyao Shen

Keyword(s):

Escherichia Coli ◽

Pichia Pastoris ◽

Nitrile Hydratase ◽

Functional Expression ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Nocardia Sp

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Oxygen evolution from water. Potential metal ligands in photosynthetic water oxidation identified by site-directed mutagenesis of Synechocystis sp. PCC 6803

Berichte der Bunsengesellschaft für physikalische Chemie ◽

10.1002/bbpc.19961001209 ◽

1996 ◽

Vol 100 (12) ◽

pp. 1978-1986

Author(s):

Neil R. Bowlby ◽

Idah Sithole ◽

Gerald T. Babcock ◽

Lee McIntosh

Keyword(s):

Water Potential ◽

Oxygen Evolution ◽

Water Oxidation ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Metal Ligands ◽

Photosynthetic Water Oxidation ◽

Synechocystis Sp ◽

Pcc 6803

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Constitutive expression of human gastric lipase in Pichia pastoris and site-directed mutagenesis of key lid-stabilizing residues

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids ◽

10.1016/j.bbalip.2017.07.002 ◽

2017 ◽

Vol 1862 (10) ◽

pp. 1025-1034 ◽

Cited By ~ 4

Author(s):

Laura Sams ◽

Sawsan Amara ◽

Almahdi Chakroun ◽

Sébastien Coudre ◽

Julie Paume ◽

...

Keyword(s):

Pichia Pastoris ◽

Constitutive Expression ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Gastric Lipase

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Site-directed mutagenesis studies on subunit I of the -type cytochrome oxidase of : a brief review of progress to date

Biochimica et Biophysica Acta (BBA) - Bioenergetics ◽

10.1016/s0005-2728(05)80015-5 ◽

1992 ◽

Vol 1101 (2) ◽

pp. 184-187

Author(s):

R GENNIS

Keyword(s):

Cytochrome Oxidase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Identification of amino acid residues in the C-terminal domain of the natriuretic peptide clearance receptor (NPR-C) that determine G protein coupling by site-directed mutagenesis

Gastroenterology ◽

10.1016/s0016-5085(01)82530-0 ◽

2001 ◽

Vol 120 (5) ◽

pp. A510-A510

Author(s):

H ZHOU ◽

K MURTHY

Keyword(s):

Amino Acid ◽

G Protein ◽

Natriuretic Peptide ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Amino Acid Residues ◽

G Protein Coupling ◽

Protein Coupling ◽

Terminal Domain ◽

Clearance Receptor

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