Comparative substrate specificity analysis of recombinant human cathepsin V and cathepsin L

2004 ◽  
Vol 430 (2) ◽  
pp. 274-283 ◽  
Author(s):  
Luciano Puzer ◽  
Simone S. Cotrin ◽  
Marcio F.M. Alves ◽  
Tobore Egborge ◽  
Mariana S. Araújo ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cathepsin L ◽  
Human Cathepsin ◽  
Cathepsin V

Expression of human cathepsin L or human cathepsin V in mouse thymus mediates positive selection of T helper cells in cathepsin L knock-out mice

Biochimie ◽  
2010 ◽  
Vol 92 (11) ◽  
pp. 1674-1680 ◽  
Author(s):  
Lisa Sevenich ◽  
Sascha Hagemann ◽  
Christina Stoeckle ◽  
Eva Tolosa ◽  
Christoph Peters ◽  
...  
Keyword(s):  
Positive Selection ◽  
T Helper Cells ◽  
Cathepsin L ◽  
T Helper ◽  
Knock Out ◽  
Helper Cells ◽  
Human Cathepsin ◽  
Knock Out Mice ◽  
Cathepsin V ◽  
Selection Of

scholarly journals Characterization of the substrate specificity of the major cysteine protease (cruzipain) from Trypanosoma cruzi using a portion-mixing combinatorial library and fluorogenic peptides

1997 ◽  
Vol 323 (2) ◽  
pp. 427-433 ◽  
Author(s):  
Elaine DEL NERY ◽  
Maria A. JULIANO ◽  
Morten MELDAL ◽  
Ib SVENDSEN ◽  
Julio SCHARFSTEIN ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Substrate Specificity ◽  
Solid Phase ◽  
Cathepsin L ◽  
Amino Acid Residues ◽  
General Preference ◽  
Human Cathepsin ◽  
Hydrolysis Of ◽  
Positively Charged

The substrate specificity of the major cysteinyl proteinase of the parasitic protozoan Trypanosoma cruzi (cruzipain) was investigated, by combinatorial replacement of amino acid residues at positions P5–P´5, using a fluorescent quenched solid-phase library assay. Positively charged residues appear to be a general preference in the P5–P3 and the P´5–P´3 positions, while a hydrophobic residue was always required at the P2 position. A broad range of amino acids could be accepted at the P´1 position. A clear difference in terms of specificity between cruzipain and human cathepsin L was observed for the accommodation of Pro at the P2 position. The P1 specificity was investigated by a more detailed enzyme kinetic analysis using peptidyl-MCA (where MCA is methylcoumarin amide) and Abz-peptidyl-EDDnp [where Abz is o-aminobenzoic acid and EDDnp is N-(2,4-dinitrophenyl)ethylenediamine] as substrates, and the results were compared with those obtained using human cathepsin L. Cruzipain showed a clear preference for benzyl-Cys or Arg at the P1 position. Human cathepsin L presented similar behaviour to that of cruzipain for the hydrolysis of the ε-NH2-Cap-Leu-Xaa-MCA (where Cap is ε-aminocaproyl) and Abz-Lys-Leu-Xaa-Phe-Ser-Lys-Gln-EDDnp series, whereas the mammalian enzyme was able to tolerate large P1 residues, such as phenylalanine, better than cruzipain in the latter series.

Biphasic modulation of human cathepsin L by a novel cyanobacterial depsipeptide

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
B Miller ◽  
M Bertin ◽  
V Hook ◽  
WH Gerwick
Keyword(s):  
Cathepsin L ◽  
Human Cathepsin

Carboxy-monopeptidase substrate specificity of human cathepsin X

2005 ◽  
Vol 329 (2) ◽  
pp. 445-452 ◽  
Author(s):  
Gopal Devanathan ◽  
Joanne L. Turnbull ◽  
Edmund Ziomek ◽  
Enrico O. Purisima ◽  
Robert Ménard ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cathepsin X ◽  
Human Cathepsin

scholarly journals Highly potent inhibitors of human cathepsin L identified by screening combinatorial pentapeptide amide collections

2000 ◽  
Vol 267 (16) ◽  
pp. 5085-5092 ◽  
Author(s):  
Achim Brinker ◽  
Ekkehard Weber ◽  
Dieter Stoll ◽  
Jürgen Voigt ◽  
Annett Müller ◽  
...  
Keyword(s):  
Cathepsin L ◽  
Human Cathepsin

Characterization and Crystallization of Recombinant Human Cathepsin L

1996 ◽  
Vol 228 (3) ◽  
pp. 792-796 ◽  
Author(s):  
Toshiyuki Nomura ◽  
Akira Fujishima ◽  
Yukio Fujisawa
Keyword(s):  
Cathepsin L ◽  
Human Cathepsin

Anti-Cathepsin L Monoclonal Antibodies That Distinguish Cathepsin L from Cathepsin V

2001 ◽  
Vol 382 (5) ◽  
pp. 867-870 ◽  
Author(s):  
Nataša Kopitar-Jerala ◽  
Tadeja Bevec ◽  
Darja Barlič-Maganja ◽  
Franc Gubenšek ◽  
Vito Turk
Keyword(s):  
Monoclonal Antibodies ◽  
Cathepsin L ◽  
Cathepsin V

scholarly journals Human Cathepsin V Protease Participates in Production of Enkephalin and NPY Neuropeptide Neurotransmitters

2012 ◽  
Vol 287 (19) ◽  
pp. 15232-15241 ◽  
Author(s):  
Lydiane Funkelstein ◽  
W. Douglas Lu ◽  
Britta Koch ◽  
Charles Mosier ◽  
Thomas Toneff ◽  
...  
Keyword(s):  
Human Cathepsin ◽  
Cathepsin V

Mercurial activation of human cathepsin L

1985 ◽  
Vol 13 (6) ◽  
pp. 1157-1157
Author(s):  
ROBERT W. MASON
Keyword(s):  
Cathepsin L ◽  
Human Cathepsin
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