The requirement of ammonium or other cations linked with p-cresol sulfate for cross-reactivity with a peptide of myelin basic protein

2003 ◽  
Vol 418 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Patricia L Jackson ◽  
Ligong Cao ◽  
J.Edwin Blalock ◽  
John N Whitaker
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Cross Reactivity

scholarly journals HIV-Infected Patients: Cross Site-Specific Hydrolysis of H3 and H4 Histones and Myelin Basic Protein with Antibodies against These Three Proteins

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 316
Author(s):  
Svetlana V. Baranova ◽  
Pavel S. Dmitrenok ◽  
Valentina N. Buneva ◽  
Sergey E. Sedykh ◽  
Georgy A. Nevinsky
Keyword(s):  
Myelin Basic Protein ◽  
Intercellular Space ◽  
Basic Protein ◽  
Cross Reactivity ◽  
Cleavage Sites ◽  
Site Specific ◽  
Protein Clusters ◽  
Negative Role ◽  
Toxic Responses ◽  
Hydrolysis Of

Histones play important roles in chromatin functioning and gene transcription, but in the intercellular space, they are harmful since they stimulate systemic inflammatory and toxic responses. Electrophoretically homogeneous IgGs against myelin basic protein (MBP), as well as H3 and H4 histones, were isolated from sera of HIV-infected patients. In contrast to known classical proteases, these IgGs split exclusively only histones and MBP but no other control proteins. Among 13 sites of hydrolysis of H3 by IgGs against H3 and 14 sites for anti-MBP IgGs, only two sites of the hydrolysis were the same. Between seven cleavage sites of H4 with IgGs against H4 and 9 sites of this histone hydrolysis by antibodies against MBP, only three sites were the same. The sites of hydrolysis of H3 (and H4) with abzymes against these histones and against MBP were different, but several expended protein clusters containing hydrolysis sites are partially overlapped. The existence of enzymatic cross-reactivity of abzymes against H3 and H4 and MBP represents a great menace to humans since due to cell apoptosis, histones constantly occur in human blood. They can hydrolyze MBP of the myelin sheath of axons and play a negative role in the pathogenesis of HIV-infected patients.

scholarly journals Human herpesvirus 6 and multiple sclerosis: A study of t cell cross-reactivity to viral and myelin basic protein antigens

2002 ◽  
Vol 68 (2) ◽  
pp. 268-272 ◽  
Author(s):  
Mara Cirone ◽  
Laura Cuomo ◽  
Claudia Zompetta ◽  
Stefano Ruggieri ◽  
Luigi Frati ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
T Cell ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Human Herpesvirus ◽  
Cross Reactivity ◽  
Human Herpesvirus 6 ◽  
Protein Antigens ◽  
Herpesvirus 6

Cross-Reactivity of Borrelia burgdorferi and Myelin Basic Protein-Specific T Cells Is Not Observed in Borrelial Encephalomyelitis

1999 ◽  
Vol 194 (1) ◽  
pp. 118-123 ◽  
Author(s):  
Annette Pohl-Koppe ◽  
Eric L. Logigian ◽  
Allen C. Steere ◽  
David A. Hafler
Keyword(s):  
T Cells ◽  
Borrelia Burgdorferi ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Cross Reactivity

Evidence for Cross-Reactivity between Antigen Derived fromTrypanosoma cruziand Myelin Basic Protein in Experimental Chagas Disease

1998 ◽  
Vol 89 (3) ◽  
pp. 304-311 ◽  
Author(s):  
Ahmad Al-Sabbagh ◽  
Celia A.A.C. Garcia ◽  
Blanca M. Diaz-Bardales ◽  
Cristiane Zaccarias ◽  
Julia Keiko Sakurada ◽  
...  
Keyword(s):  
Myelin Basic Protein ◽  
Chagas Disease ◽  
Basic Protein ◽  
Cross Reactivity

scholarly journals Immunohistochemical application of monoclonal antibodies against myelin basic protein and neurofilament triple protein subunits: advantages over antisera and technical limitations.

1983 ◽  
Vol 31 (9) ◽  
pp. 1126-1135 ◽  
Author(s):  
W F Hickey ◽  
V Lee ◽  
J Q Trojanowski ◽  
L J McMillan ◽  
T J McKearn ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Brain ◽  
Myelin Basic Protein ◽  
Brain Tissue ◽  
Mercuric Chloride ◽  
Basic Protein ◽  
Histological Study ◽  
Cross Reactivity ◽  
Human Brain Tissue ◽  
Specific Staining

Four monoclonal antibodies against guinea pig myelin basic protein (MBP), and four against subunits of bovine neurofilament triplet proteins (NF) were produced and their activity determined by enzyme-linked immunosorbant assay. The specificity and cross-reactivity of these eight monoclonal antibodies and one heterologous antiserum against each of the two central nervous system (CNS) antigens were examined in a histological study using the immunoperoxidase, antibody sandwich technique in rat and human brain tissue. Tissue sections were prepared from paraffin-embedded or fresh brain tissue that had been fixed with one of five different fixatives. The resulting immunoperoxidase labeling was then graded for intensity and examined for artifacts. One monoclonal antibody against MBP and one against NF resulted in labeling that was superior to that given by each of the antisera against their respective antigens. Of the five fixatives tested, a mercuric chloride-formalin solution gave the best preservation of these two antigens in rat and human brain tissue. The mercuric chloride-formalin solution was found to be superior to the other fixatives when immersion fixation was used, and was especially optimal when brains were perfused fixed. Three artifacts were encountered among the various antibody-fixative combinations that produced erroneous, but seemingly specific staining of Purkinje cells, neurons and axons, or astrocytes.

Immunochemical cross-reactivity between intact purified myelin basic protein (MBP) and the synthetic encephalitogenic peptide S49

1984 ◽  
Vol 9 (9) ◽  
pp. 1295-1308 ◽  
Author(s):  
Kenneth J. Lazarus ◽  
George A. Hashim ◽  
Philip Y. Paterson ◽  
Eugene D. Day
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Cross Reactivity
Sign in / Sign up

Export Citation Format

Share Document