Selection and application of broad-specificity human domain antibody for simultaneous detection of Bt Cry toxins

2016 ◽  
Vol 512 ◽  
pp. 70-77 ◽  
Author(s):  
Chongxin Xu ◽  
Xiao Zhang ◽  
Xiaoqin Liu ◽  
Yuan Liu ◽  
Xiaodan Hu ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Cry Toxins ◽  
Domain Antibody ◽  
Broad Specificity

Engineering of a Broad Specificity Antibody for Simultaneous Detection of 13 Sulfonamides at the Maximum Residue Level

2004 ◽  
Vol 52 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Teemu Korpimäki ◽  
Eeva-Christine Brockmann ◽  
Outi Kuronen ◽  
Maija Saraste ◽  
Urpo Lamminmäki ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Residue Level ◽  
Maximum Residue Level ◽  
Broad Specificity

Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay

2016 ◽  
Vol 502 ◽  
pp. 1-7 ◽  
Author(s):  
Yanyan Zhao ◽  
Ying Liang ◽  
Yuan Liu ◽  
Xiao Zhang ◽  
Xiaodan Hu ◽  
...  
Keyword(s):  
Phage Library ◽  
Domain Antibody ◽  
Specificity Domain ◽  
Broad Specificity

Broad-Specificity Immunoassay for Simultaneous Detection of Ochratoxins A, B, and C in Millet and Maize

2017 ◽  
Vol 65 (23) ◽  
pp. 4830-4838 ◽  
Author(s):  
Yaqiong Zhang ◽  
Lanteng Wang ◽  
Xing Shen ◽  
Xiaoqun Wei ◽  
Xinan Huang ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Broad Specificity

Simultaneous detection of parathion and imidacloprid using broad-specificity polyclonal antibody in enzyme-linked immunosorbent assay

2015 ◽  
Vol 7 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Lening Zhang ◽  
Zhanfeng Wang ◽  
Yubin Wen ◽  
Jingwei Shi ◽  
Jincheng Wang
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Simultaneous Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Broad Specificity

A heterologous indirect competitive enzyme-linked immunosorbent assay for the gross determination of parathion and imidacloprid residues was developed.

Simultaneous detection of multiple chemical residues in milk using broad-specificity antibodies in a hybrid immunosorbent assay

2011 ◽  
Vol 26 (5) ◽  
pp. 2716-2719 ◽  
Author(s):  
Kui Zhu ◽  
Jiancheng Li ◽  
Zhanhui Wang ◽  
Haiyang Jiang ◽  
Ross C. Beier ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Simultaneous Detection ◽  
Chemical Residues ◽  
Multiple Chemical ◽  
Broad Specificity

Species Specific Immunoassays to Measure Blood Platelet and Coagulation Activation in the Rat

1996 ◽  
Vol 76 (06) ◽  
pp. 1090-1095 ◽  
Author(s):  
C Ravanat ◽  
M Freund ◽  
S Schuhler ◽  
P Grunert ◽  
L Meyer ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Plasma Concentrations ◽  
Simultaneous Detection ◽  
Blood Platelet ◽  
Coagulation Activation ◽  
Platelet Factor ◽  
Prethrombotic State ◽  
Low Levels ◽  
Species Specific ◽  
Higher Sensitivity

SummaryThe purpose of this study was to develop specific and sensitive immunoassays to detect early indices of hypercoagulability in the rat. Rat platelet factor 4 (rPF4) and rat fibrinopeptide A (rFPA) assays, tools for the detection of activation of platelets and coagulation respectively, were designed using antibodies raised against purified rPF4 and against synthetic rFPA. The relevance of these new assays and of the commercially available ELISA kit for thrombin-antithrombin III (TAT) complexes was demonstrated in a rat model of a prethrombotic state induced by intravenous infusion of varying doses of thromboplastin (90 to 2400 μl/kg/h). In this model, the immunoassays allowed simultaneous detection of low levels of rFPA and rPF4 which were correlated with fibrinogen and platelet consumption and TAT generation and further proved to be of higher sensitivity than the classical methods of platelet count or measurement of fibrinogen levels. Plasma concentrations of rFPA, rPF4 and TAT were dependent on infusion time and thromboplastin dose, while hirudin (1 mg/kg) prevented their appearance. Thus the new specific immunoassays for rPF4 and rFPA and the commercial human TAT assay represent useful tools for pathophysiological studies or the screening of antithrombotic drugs in rats.

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