scholarly journals High-performance targeted mass spectrometry with precision data-independent acquisition reveals site-specific glycosylation macroheterogeneity

2016 ◽  
Vol 510 ◽  
pp. 106-113 ◽  
Author(s):  
K.Y. Benjamin Yeo ◽  
Panagiotis K. Chrysanthopoulos ◽  
Amanda S. Nouwens ◽  
Esteban Marcellin ◽  
Benjamin L. Schulz
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Precision Data ◽  
Site Specific ◽  
Data Independent Acquisition

scholarly journals Deep ubiquitination site profiling by single-shot data-independent acquisition mass spectrometry

2020 ◽  
Author(s):  
Martin Steger ◽  
Phillip Ihmor ◽  
Mattias Backman ◽  
Stefan Müller ◽  
Henrik Daub
Keyword(s):  
Mass Spectrometry ◽  
High Precision ◽  
Protein Extraction ◽  
Single Shot ◽  
Spectral Library ◽  
Sample Analysis ◽  
Precision Data ◽  
Data Independent Acquisition ◽  
Ubiquitination Site ◽  
High Precision Data

We report a highly optimized proteomics method for in-depth ubiquitination profiling, which combines efficient protein extraction and data-independent acquisition mass spectrometry (DIA-MS). Employing DIA for both spectral library generation and single-shot sample analysis, we quantify up to 70,000 ubiquitinated peptides per MS run with high precision, data completeness and throughput. Our approach resolves the dynamics of ubiquitination and protein degradation with an unprecedented analytical depth.

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