A strategy to quantitate global phosphorylation of bone matrix proteins

2016 ◽  
Vol 499 ◽  
pp. 85-89 ◽  
Author(s):  
Grażyna E. Sroga ◽  
Deepak Vashishth
Keyword(s):  
Bone Matrix ◽  
Matrix Proteins ◽  
Bone Matrix Proteins

scholarly journals Effect of protein restriction on the messenger RNA contents of bone-matrix proteins, insulin-like growth factors and insulin-like growth factor binding proteins in femur of ovariectomized rats

1996 ◽  
Vol 75 (6) ◽  
pp. 811-823 ◽  
Author(s):  
Yusuke Higashi ◽  
Asako Takenaka ◽  
Shin-Ichiro Takahashi ◽  
Tadashi Noguchi
Keyword(s):  
Dietary Protein ◽  
Type I Collagen ◽  
Control Diet ◽  
Bone Matrix ◽  
Protein Restriction ◽  
Matrix Proteins ◽  
Type I ◽  
Bone Matrix Proteins ◽  
Mrna Content ◽  
Insulin Like Growth Factors

It has been reported that loss of ovarian oestrogen after menopause or by ovariectomy causes osteoporosis. In order to elucidate the effect of dietary protein restriction on bone metabolism after ovariectomy, we fed ovariectomized young female rats on a casein-based diet (50g/kg diet (protein restriction) or 200g/kg diet (control)) for 3 weeks and measured mRNA contents of bone-matrix proteins such as osteocalcin, osteopontin and α1 type I collagen, insulin-like growth factors (IGF) and IGF-binding proteins (IGFBP) in femur. Ovariectomy decreased the weight of fat-free dry bone and increased urinary excretion of pyridinium cross-links significantly, although dietary protein restriction did not affect them. Neither ovariectomy nor protein restriction affected the content of mRNA of osteopontin and osteocalcin; however, ovariectomy increased and protein restriction extensively decreased the α1 type I collagen mRNA content in bone tissues. Ovariectomy increased IGF-I mRNA only in the rats fed on the control diet. Conversely, protein rest riction increased and ovariectomy decreased the IGF-II mRNA content in femur. Furthermore, the contents of IGFBP-2, IGFBP-4 and IGFBP-5 mRNA increased, but the content of IGFBP-3 mRNA decreased in femur of the rats fed on the protein-restricted diet. In particular, ovariectomy decreased the IGFBP-2 mRNA content in the protein-restricted rats and the IGFBP-6 mRNA content in the rats fed on the control diet. These results clearly show that the mRNA for some of the proteins which have been shown to be involved in bone formation are regulated by both quantity of dietary proteins and ovarian hormones.

Expression of bone matrix proteins during the osseus healing of topical conditioned implants: an experimental study

2006 ◽  
Vol 17 (6) ◽  
pp. 666-672 ◽  
Author(s):  
Karl Andreas Schlegel ◽  
Michael Thorwarth ◽  
Alexandra Plesinac ◽  
Joerg Wiltfang ◽  
Stephan Rupprecht
Keyword(s):  
Experimental Study ◽  
Bone Matrix ◽  
Matrix Proteins ◽  
Bone Matrix Proteins

Induction of Bone Matrix Protein Expression by Native Bone Matrix Proteins in C2C12 Culture

2009 ◽  
Vol 22 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Zhen-Ming HU ◽  
Sean A.F. PEEL ◽  
Stephen K.C. HO ◽  
George K.B. SANDOR ◽  
Cameron M.L. CLOKIE
Keyword(s):  
Protein Expression ◽  
Matrix Protein ◽  
Bone Matrix ◽  
Matrix Proteins ◽  
Native Bone ◽  
Bone Matrix Proteins ◽  
Bone Matrix Protein

scholarly journals Osteomimicry of Mammary Adenocarcinoma Cells In Vitro; Increased Expression of Bone Matrix Proteins and Proliferation within a 3D Collagen Environment

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41679 ◽  
Author(s):  
Rachel F. Cox ◽  
Allan Jenkinson ◽  
Kerstin Pohl ◽  
Fergal J. O’Brien ◽  
Maria P. Morgan
Keyword(s):  
Bone Matrix ◽  
Matrix Proteins ◽  
Mammary Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Bone Matrix Proteins ◽  
3D Collagen

scholarly journals Biochemical Characterization of Major Bone-Matrix Proteins Using Nanoscale-Size Bone Samples and Proteomics Methodology

2011 ◽  
Vol 10 (9) ◽  
pp. M110.006718 ◽  
Author(s):  
Grażyna E. Sroga ◽  
Lamya Karim ◽  
Wilfredo Colón ◽  
Deepak Vashishth
Keyword(s):  
Biochemical Characterization ◽  
Bone Matrix ◽  
Matrix Proteins ◽  
Bone Matrix Proteins
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