Micromolar colorimetric detection of 2-hydroxy ketones with the water-soluble tetrazolium WST-1

2016 ◽  
Vol 493 ◽  
pp. 8-10 ◽  
Author(s):  
Martin Bommer ◽  
John M. Ward
The Analyst ◽  
2013 ◽  
Vol 138 (6) ◽  
pp. 1649 ◽  
Author(s):  
Zhiyi Yao ◽  
Binghuan Huang ◽  
Xianping Hu ◽  
Li Zhang ◽  
Depeng Li ◽  
...  

2018 ◽  
Vol 5 (18) ◽  
pp. 2641-2651 ◽  
Author(s):  
Annike Weißenstein ◽  
Vincenzo Grande ◽  
Chantu R. Saha-Möller ◽  
Frank Würthner

Biocompatible water-soluble naphthalene diimides (NDIs) were synthesized and a core-dichlorinated NDI was shown to detect primary amines and biogenic diamines.


2015 ◽  
Vol 51 (40) ◽  
pp. 8544-8546 ◽  
Author(s):  
Dandan Cheng ◽  
Yandong Li ◽  
Jing Wang ◽  
Yujiao Sun ◽  
Lu Jin ◽  
...  

A sensitive fluorescent and colorimetric dual-modal probe for the detection of ATP has been developed based on a strategy of self-promoting aggregation of a cationic polythiophene derivative bearing anthracene groups in the side chain with a detection limit as low as 10−9 M.


2014 ◽  
Vol 6 (11) ◽  
pp. 3784-3790 ◽  
Author(s):  
Subhenjit Hazra ◽  
Shruti Balaji ◽  
Mainak Banerjee ◽  
Anasuya Ganguly ◽  
Narendra Nath Ghosh ◽  
...  

A water soluble turn-on fluorescent probe for the detection of mercury ions in purely aqueous media a has been developed by appending a water-compatible PEG-unit onto a rhodamine derivative. It has high selectivity for Hg2+ ions and is fairly non-toxic in intercellular studies.


Author(s):  
J. G. Robertson ◽  
D. F. Parsons

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.


Author(s):  
J. D. McLean ◽  
S. J. Singer

The successful application of ferritin labeled antibodies (F-A) to ultrathin sections of biological material has been hampered by two main difficulties. Firstly the normally used procedures for the preparation of material for thin sectioning often result in a loss of antigenicity. Secondly the polymers employed for embedding may non-specifically absorb the F-A. Our earlier use of cross-linked polyampholytes as embedding media partially overcame these problems. However the water-soluble monomers used for this method still extract many lipids from the material.


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