scholarly journals High-performance liquid chromatography-based method to evaluate kinetics of glucosinolate hydrolysis by Sinapis alba myrosinase

2014 ◽  
Vol 465 ◽  
pp. 105-113 ◽  
Author(s):  
Kayla J. Vastenhout ◽  
Ruthellen H. Tornberg ◽  
Amanda L. Johnson ◽  
Michael W. Amolins ◽  
Jared R. Mays
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Sinapis Alba ◽  
Kinetics Of

Ceftazidime kinetics of diffusion in inoculated agar plates.

1996 ◽  
Vol 40 (5) ◽  
pp. 1280-1281 ◽  
Author(s):  
C Arcelloni ◽  
R Paroni ◽  
M Basile ◽  
P A Bonini ◽  
R Vaiani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Fixed Points ◽  
High Performance ◽  
Statistical Difference ◽  
Disk Center ◽  
Beta Lactamase ◽  
Fixed Times ◽  
Kinetics Of

The ceftazidime concentration in agar plates inoculated with Pseudomonas aeruginosa was determined by high-performance liquid chromatography at fixed points (3, 6, 9, 12, and 15 mm) from the disk center and at fixed times (2, 4, 6, 16, and 24 h) to study the antibiotic kinetics of diffusion. A statistical difference between the concentrations determined in the presence of microorganisms and in uninoculated plates after 16 and 24 h was evidenced and was probably ascribable to the drug hydrolysis carried out by the induced beta-lactamase.

Vulcanization Chemistry. Sulfur, Nt-Butyl-2-Benzothiazole Sulfenamide Formulations Studied by High Performance Liquid Chromatography

1992 ◽  
Vol 65 (2) ◽  
pp. 488-501 ◽  
Author(s):  
A. B. Sullivan ◽  
Cynthia J. Hann ◽  
George H. Kuhls
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Material Balance ◽  
Side Reaction ◽  
Analytical Methodology ◽  
Wide Range ◽  
Kinetics Of ◽  
Vulcanization Process ◽  
Intermediate Concentration

Abstract The paper reports on the sulfur vulcanization chemistry of cis-polyisoprene formulations accelerated with N-t-butyl-2-benzothiazole sulfenamide. High Performance Liquid Chromatography (HPLC) is utilized to examine the kinetics of accelerator—sulfur disappearance and the formation—disappearance profiles of extra-network cure intermediates across the vulcanization process. Cure profile measurements are reported on three formulations covering a wide range of sulfur—accelerator ratios. Also unique aspects of the analytical methodology are highlighted. The observed cure-intermediate concentration profiles reinforce earlier theories about the chemical details of sulfurization and subsequent crosslinking. On all formulations, benzothiazole polysulfides (S1−S4) reach a maximum concentration at the scorch midpoint coincident with slope changes in reactant and product profiles. Although pendant-accelerator crosslink precursors are not measured directly, their presence is implied from material balance calculations. Also, the formation of a small amount of benzothiazole early in the scorch period is a new observation in sulfenamide acceleration. It suggests that accelerator hydrolysis is a competitive side reaction early in the vulcanization process.

Sign in / Sign up

Export Citation Format

Share Document