scholarly journals A direct and continuous assay for the determination of thioredoxin reductase activity in cell lysates

2013 ◽  
Vol 443 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Brian Cunniff ◽  
Gregg W. Snider ◽  
Nicholas Fredette ◽  
Robert J. Hondal ◽  
Nicholas H. Heintz
Keyword(s):  
Reductase Activity ◽  
Thioredoxin Reductase ◽  
Cell Lysates ◽  
Continuous Assay

Determination of Thioredoxin Reductase Activity in Rat Liver Supernatant

1997 ◽  
Vol 253 (1) ◽  
pp. 123-125 ◽  
Author(s):  
Kristina E. Hill ◽  
Gary W. McCollum ◽  
Raymond F. Burk
Keyword(s):  
Rat Liver ◽  
Reductase Activity ◽  
Thioredoxin Reductase

scholarly journals Fluorogenic probes for thioredoxin reductase activity

2021 ◽  
Vol 3 ◽  
pp. 100127
Author(s):  
Tendai J. Mafireyi ◽  
Jorge O. Escobedo ◽  
Robert M. Strongin
Keyword(s):  
Reductase Activity ◽  
Thioredoxin Reductase ◽  
Fluorogenic Probes

Determination of AL01576 Concentration in Rat Lenses and Plasma by Bioassay for Aldose Reductase Activity Measurements

1989 ◽  
Vol 21 (4) ◽  
pp. 285-291 ◽  
Author(s):  
Otto Hockwin ◽  
Peter Müller ◽  
Jan Krolczyk ◽  
Bette A. McCue ◽  
Philip R. Mayer
Keyword(s):  
Aldose Reductase ◽  
Reductase Activity ◽  
Activity Measurements

scholarly journals Inhibition of Glutathione and Thioredoxin Metabolism Enhances Sensitivity to Perifosine in Head and Neck Cancer Cells

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
Andrean L. Simons ◽  
Arlene D. Parsons ◽  
Katherine A. Foster ◽  
Kevin P. Orcutt ◽  
Melissa A. Fath ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Reductase Activity ◽  
Thioredoxin Reductase ◽  
Buthionine Sulfoximine ◽  
Human Head ◽  
Akt Inhibitor ◽  
Simultaneous Treatment

The hypothesis that the Akt inhibitor, perifosine (PER), combined with inhibitors of glutathione (GSH) and thioredoxin (Trx) metabolism will induce cytotoxicity via metabolic oxidative stress in human head and neck cancer (HNSCC) cells was tested. PER induced increases in glutathione disulfide (%GSSG) in FaDu, Cal-27, and SCC-25 HNSCCs as well as causing significant clonogenic cell killing in FaDu and Cal-27, which was suppressed by simultaneous treatment with N-acetylcysteine (NAC). An inhibitor of GSH synthesis, buthionine sulfoximine (BSO), sensitized Cal-27 and SCC-25 cells to PER-induced clonogenic killing as well as decreased total GSH and increased %GSSG. Additionally, inhibition of thioredoxin reductase activity (TrxRed) with auranofin (AUR) was able to induce PER sensitization in SCC-25 cells that were initially refractory to PER. These results support the conclusion that PER induces oxidative stress and clonogenic killing in HNSCC cells that is enhanced with inhibitors of GSH and Trx metabolism.

Recombinant putative glutathione reductase of Plasmodium falciparum exhibits thioredoxin reductase activity

1996 ◽  
Vol 80 (2) ◽  
pp. 215-219 ◽  
Author(s):  
Sylke Müller ◽  
Tim-Wolf Gilberger ◽  
Petra M. Färber ◽  
Katja Becker ◽  
R. Heiner Schirmer ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Thioredoxin Reductase
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