Loop region-specific oligonucleotide probes for loop-mediated isothermal amplification–enzyme-linked immunosorbent assay truly minimize the instrument needed for detection process

2013 ◽  
Vol 439 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Hadi Ravan ◽  
Razieh Yazdanparast
Keyword(s):  
Immunosorbent Assay ◽  
Isothermal Amplification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oligonucleotide Probes ◽  
Detection Process ◽  
Loop Mediated Isothermal Amplification ◽  
Loop Region

scholarly journals Rapid diagnosis of Toxoplasma gondii using loop-mediated isothermal amplification assay in camels and small ruminants

2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Gamil S. G. Zeedan ◽  
Abeer M. Abdalhamed ◽  
Raafat M. Shaapan ◽  
Amira H. El-Namaky
Keyword(s):  
Small Ruminants ◽  
Lamp Assay ◽  
Laboratory Diagnosis ◽  
Isothermal Amplification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Blood Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Detection Capabilities

Abstract Background This study was conducted to detect the presence of T. gondii in milk and blood samples using three different assays: enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and loop-mediated isothermal amplification assay (LAMP). Whole blood, serum, and milk samples were collected from goats (n = 156), sheep (n = 261), and camels (n = 108) in different governorates in Egypt from December 2019 to February 2021 and screened by ELISA for anti-Toxoplasma IgG antibodies before DNA extraction. The target T. gondii DNA gene was detected and evaluated using the LAMP assay compared to PCR. Results T. gondii antibodies were found in milk and serum samples at the rates of (29.26%) and (36.58%) in camels, (34.18%) and (35.89%) in sheep, and (33.7%) and (36.36%) in goats, respectively. Similar to PCR, the percentages of LAMP tests for the detection of the T. gondii DNA gene in milk and blood samples of camels, sheep, and goats were (4.8, 14.63), (6.83, 7.69), and (7.79, 9.09), respectively. LAMP's sensitivity for detecting T. gondii in milk and blood samples, which was identical to that of PCR, was 100%. Conclusions The findings clearly demonstrated that there were no variations in T. gondii detection capabilities in milk and blood samples from various animals using both PCR and LAMP tests. It provides a quick, precise, and sensitive method of detecting T. gondii in a variety of samples that may be used both in the field and in laboratory diagnosis.

Utility of loop-mediated isothermal amplification as a point-of-care test in diagnosis of amoebic liver abscess

2021 ◽  
pp. 004947552110180
Author(s):  
Dipti Handa ◽  
Monica Gupta ◽  
Sarabmeet Singh Lehl ◽  
Amit Gupta ◽  
Ram Singh
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Liver Abscess ◽  
Point Of Care ◽  
Isothermal Amplification ◽  
Amoebic Liver Abscess ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Loop Mediated Isothermal Amplification ◽  
Polymerase Chain

Definitive diagnosis of amoebic liver abscess is challenging owing to the unavailability of sensitive commercial point-of-care molecular tests. The primary aim of our prospective diagnostic study was to compare available laboratory methods for the diagnosis of Entamoeba histolytica in clinical samples with loop-mediated isothermal amplification. We compared deoxyribonucleic acid (DNA) analysis methods, namely, loop-mediated isothermal amplification and reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) using pus, stool and blood samples from 200 patients with clinical and radiological diagnosis of amoebic liver abscess. Loop-mediated isothermal amplification had significantly higher sensitivity (88%) as compared to reverse transcriptase polymerase chain reaction (64%) and excellent specificity (100%).

Sign in / Sign up

Export Citation Format

Share Document