A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol

Deguang Wu; Xuewu Guo; Jun Lu; Xi Sun; Feng Li; Yefu Chen; Dongguang Xiao

doi:10.1016/j.ab.2012.11.028

A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol

Analytical Biochemistry ◽

10.1016/j.ab.2012.11.028 ◽

2013 ◽

Vol 434 (2) ◽

pp. 254-258 ◽

Cited By ~ 11

Author(s):

Deguang Wu ◽

Xuewu Guo ◽

Jun Lu ◽

Xi Sun ◽

Feng Li ◽

...

Keyword(s):

One Step ◽

Step Site

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One-step site-specific antibody fragment auto-conjugation using SNAP-tag technology

Nature Protocols ◽

10.1038/s41596-019-0214-y ◽

2019 ◽

Vol 14 (11) ◽

pp. 3101-3125 ◽

Cited By ~ 3

Author(s):

Ahmad Fawzi Hussain ◽

Paul A. Heppenstall ◽

Florian Kampmeier ◽

Ivo Meinhold-Heerlein ◽

Stefan Barth

Keyword(s):

Specific Antibody ◽

Antibody Fragment ◽

Site Specific ◽

One Step ◽

Step Site

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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

BMC Biotechnology ◽

10.1186/1472-6750-8-91 ◽

2008 ◽

Vol 8 (1) ◽

pp. 91 ◽

Cited By ~ 481

Author(s):

Huanting Liu ◽

James H Naismith

Keyword(s):

Multiple Site ◽

One Step ◽

Step Site

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Broad substrate tolerance of tubulin tyrosine ligase enables one-step site-specific enzymatic protein labeling

Chemical Science ◽

10.1039/c7sc00574a ◽

2017 ◽

Vol 8 (5) ◽

pp. 3471-3478 ◽

Cited By ~ 20

Author(s):

Dominik Schumacher ◽

Oliver Lemke ◽

Jonas Helma ◽

Lena Gerszonowicz ◽

Verena Waller ◽

...

Keyword(s):

Protein Labeling ◽

Site Specific ◽

Wide Applicability ◽

One Step ◽

Tubulin Tyrosine Ligase ◽

Protein Functionalization ◽

Substrate Tolerance ◽

Step Site

The broad substrate tolerance of tubulin tyrosine ligase enables its wide applicability for protein functionalization.

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One-Step Site-Specific Activation Approach for Preparation of Hierarchical Porous Carbon Materials with High Electrochemical Performance

ACS Applied Energy Materials ◽

10.1021/acsaem.9b01729 ◽

2019 ◽

Vol 2 (12) ◽

pp. 8767-8782 ◽

Cited By ~ 2

Author(s):

Zongze Lv ◽

Xiaoli Li ◽

Xiujuan Chen ◽

Xiang Li ◽

Meng Wu ◽

...

Keyword(s):

Electrochemical Performance ◽

Porous Carbon ◽

Carbon Materials ◽

Hierarchical Porous Carbon ◽

Site Specific ◽

Porous Carbon Materials ◽

Hierarchical Porous ◽

One Step ◽

Hierarchical Porous Carbon Materials ◽

Step Site

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Faculty Opinions recommendation of An efficient one-step site-directed and site-saturation mutagenesis protocol.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1027208.327649 ◽

2005 ◽

Author(s):

Karen Allen

Keyword(s):

Saturation Mutagenesis ◽

Site Saturation ◽

One Step ◽

Step Site

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One-step site-specific modification of native proteins with 2-pyridinecarboxyaldehydes

Nature Chemical Biology ◽

10.1038/nchembio.1792 ◽

2015 ◽

Vol 11 (5) ◽

pp. 326-331 ◽

Cited By ~ 128

Author(s):

James I MacDonald ◽

Henrik K Munch ◽

Troy Moore ◽

Matthew B Francis

Keyword(s):

Site Specific ◽

Specific Modification ◽

Native Proteins ◽

One Step ◽

Step Site

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One-step site-directed mutagenesis of viroid dimeric cDNA

Journal of Virological Methods ◽

10.1016/j.jviromet.2007.05.027 ◽

2007 ◽

Vol 145 (1) ◽

pp. 71-75 ◽

Cited By ~ 3

Author(s):

Rafael Sanjuán ◽

José-Antonio Daròs

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

One Step ◽

Step Site

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One-Step site-directed mutagenesis of ATM cDNA in large (20kb) plasmid constructs

Human Mutation ◽

10.1002/humu.9068 ◽

2002 ◽

Vol 20 (4) ◽

pp. 323-323 ◽

Cited By ~ 19

Author(s):

Shaun P. Scott ◽

Alison Teh ◽

Cheng Peng ◽

Martin F. Lavin

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

One Step ◽

Step Site

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Faculty Opinions recommendation of One-step site-specific modification of native proteins with 2-pyridinecarboxyaldehydes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725408885.793522463 ◽

2016 ◽

Author(s):

Richard Willson

Keyword(s):

Site Specific ◽

Specific Modification ◽

Native Proteins ◽

One Step ◽

Step Site

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One-step site-directed mutagenesis of the Kex2 protease oxyanion hole

Current Biology ◽

10.1016/0960-9822(93)90040-u ◽

1993 ◽

Vol 3 (8) ◽

pp. 498-506 ◽

Cited By ~ 25

Author(s):

Charles Brenner ◽

Alison Bevan ◽

Robert S. Fuller

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Oxyanion Hole ◽

One Step ◽

Kex2 Protease ◽

Step Site

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