Mechanism of Coomassie Brilliant Blue G-250 binding to cetyltrimethylammonium bromide: An interference with the Bradford assay

Mahdi Aminian; Fariba Nabatchian; Asad Vaisi-Raygani; Mojgan Torabi

doi:10.1016/j.ab.2012.11.014

Mechanism of Coomassie Brilliant Blue G-250 binding to cetyltrimethylammonium bromide: An interference with the Bradford assay

Analytical Biochemistry ◽

10.1016/j.ab.2012.11.014 ◽

2013 ◽

Vol 434 (2) ◽

pp. 287-291 ◽

Cited By ~ 15

Author(s):

Mahdi Aminian ◽

Fariba Nabatchian ◽

Asad Vaisi-Raygani ◽

Mojgan Torabi

Keyword(s):

Cetyltrimethylammonium Bromide ◽

Brilliant Blue ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Bradford Assay ◽

Coomassie Brilliant

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Protocol for the quantification of protein ng quantities by a Coomassie Brilliant Blue G-250-based hydrophobic assay

Protocol Exchange ◽

10.1038/nprot.2009.214 ◽

2009 ◽

Author(s):

Konstantinos Grintzalis ◽

Ioannis Papapostolou ◽

Christos Georgiou

Keyword(s):

Brilliant Blue ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

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Quantification of attached cells in microtiter plates based on Coomassie brilliant blue G-250 staining of total cellular protein

Analytical Biochemistry ◽

10.1016/0003-2697(84)90187-8 ◽

1984 ◽

Vol 140 (2) ◽

pp. 417-423 ◽

Cited By ~ 42

Author(s):

Craig Laughton

Keyword(s):

Cellular Protein ◽

Brilliant Blue ◽

Brilliant Blue G ◽

Total Cellular Protein ◽

Coomassie Brilliant Blue ◽

Microtiter Plates ◽

Coomassie Brilliant

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A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis

Journal of Bioscience and Bioengineering ◽

10.1263/jbb.106.111 ◽

2008 ◽

Vol 106 (1) ◽

pp. 111-113 ◽

Cited By ~ 21

Author(s):

Chun Yi Liau ◽

Chung-Sheng Lin

Keyword(s):

Molecular Weight ◽

Gel Electrophoresis ◽

Staining Method ◽

Polyacrylamide Gel Electrophoresis ◽

Chitinase Activity ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

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Aggregation of a Dye on a Surfactant: Interaction of Coomassie Brilliant Blue G250 with Cetyltrimethylammonium Bromide and Determination of the Cationic Surfactant in Water

Instrumentation Science & Technology ◽

10.1081/ci-120030539 ◽

2004 ◽

Vol 32 (3) ◽

pp. 271-280 ◽

Cited By ~ 7

Author(s):

Hong‐Wen Gao ◽

Li‐Xin Zheng ◽

Hou‐Dong Mei

Keyword(s):

Cationic Surfactant ◽

Cetyltrimethylammonium Bromide ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

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Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250

Electrophoresis ◽

10.1002/elps.1150090603 ◽

1988 ◽

Vol 9 (6) ◽

pp. 255-262 ◽

Cited By ~ 1848

Author(s):

Volker Neuhoff ◽

Norbert Arold ◽

Dieter Taube ◽

Wolfgang Ehrhardt

Keyword(s):

Isoelectric Focusing ◽

Brilliant Blue ◽

Polyacrylamide Gels ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

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Automatic assay of urinary protein using Coomassie Brilliant Blue G-250

Analytical Biochemistry ◽

10.1016/0003-2697(81)90066-x ◽

1981 ◽

Vol 113 (1) ◽

pp. 197-201 ◽

Cited By ~ 11

Author(s):

Kiyoko Sano ◽

Kiyoko Kanamori ◽

Akihiko Shiba ◽

Makoto Nakao

Keyword(s):

Urinary Protein ◽

Brilliant Blue ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

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Coomassie brilliant blue G-250 dye-binding technique for determination of autolytic protein breakdown in Euglena gracilis and comparison to other methods of autolysis measurement

Analytical Biochemistry ◽

10.1016/0003-2697(86)90088-6 ◽

1986 ◽

Vol 153 (2) ◽

pp. 242-250 ◽

Cited By ~ 17

Author(s):

Rainald Krauspe ◽

Angelika Scheer

Keyword(s):

Euglena Gracilis ◽

Brilliant Blue ◽

Protein Breakdown ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Dye Binding ◽

Coomassie Brilliant

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The staining of starch gels with Coomassie Brilliant Blue G 250 perchloric acid solution

Cellular and Molecular Life Sciences ◽

10.1007/bf01934063 ◽

1977 ◽

Vol 33 (12) ◽

pp. 1668-1668 ◽

Cited By ~ 15

Author(s):

Gail McFarland

Keyword(s):

Acid Solution ◽

Perchloric Acid ◽

Brilliant Blue ◽

Brilliant Blue G ◽

Perchloric Acid Solution ◽

Coomassie Brilliant Blue ◽

Starch Gels ◽

Coomassie Brilliant

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Protein assay by Coomassie brilliant blue G-250-binding method is unsuitable for plant tissues rich in phenols and phenolases

Analytical Biochemistry ◽

10.1016/0003-2697(87)90238-7 ◽

1987 ◽

Vol 163 (2) ◽

pp. 376-384 ◽

Cited By ~ 25

Author(s):

Roshan L. Mattoo ◽

Mohammad Ishaq ◽

M. Saleemuddin

Keyword(s):

Brilliant Blue ◽

Plant Tissues ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Protein Assay ◽

Coomassie Brilliant

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Protein staining by Coomassie Brilliant Blue G-250: Unique color rendering in iron-toned prints

Electrophoresis ◽

10.1002/elps.1150071012 ◽

1986 ◽

Vol 7 (10) ◽

pp. 482-483 ◽

Cited By ~ 1

Author(s):

Theodore O. Sippel

Keyword(s):

Brilliant Blue ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Unique Color ◽

Protein Staining ◽

Color Rendering ◽

Coomassie Brilliant

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