A microplate fluorescence assay for DAPA aminotransferase by detection of the vicinal diamine 7,8-diaminopelargonic acid

2013 ◽  
Vol 432 (2) ◽  
pp. 90-96 ◽  
Author(s):  
Stéphane Mann ◽  
Luc Eveleigh ◽  
Olivier Lequin ◽  
Olivier Ploux
Keyword(s):  
Fluorescence Assay ◽  
Microplate Fluorescence Assay

Development of a microplate fluorescence assay for kynurenine aminotransferase

2011 ◽  
Vol 409 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Jacky Wong ◽  
William J. Ray ◽  
Anna Y. Kornilova
Keyword(s):  
Fluorescence Assay ◽  
Kynurenine Aminotransferase ◽  
Microplate Fluorescence Assay

Microplate fluorescence assay for the quantification of double stranded DNA using SYBR Green I dye

2006 ◽  
Vol 28 (19) ◽  
pp. 1587-1594 ◽  
Author(s):  
Johanna Leggate ◽  
Ray Allain ◽  
Leah Isaac ◽  
Burton W. Blais
Keyword(s):  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Double Stranded Dna ◽  
Microplate Fluorescence Assay

scholarly journals Optimization of a Microplate Assay for Generating Listeria Monocytogenes, E. Coli O157:H7, and Salmonella Biofilms and Enzymatic Recovery for Enumeration

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 541 ◽  
Author(s):  
Manish Aryal ◽  
Preetty Pranatharthiharan ◽  
Peter M. Muriana
Keyword(s):  
Foodborne Pathogens ◽  
Food Processing ◽  
Hydrolytic Enzymes ◽  
Fluorescence Assay ◽  
Escherichia Coli O157 ◽  
Bacterial Cells ◽  
Planktonic Cells ◽  
E Coli ◽  
Adherence Data ◽  
Microplate Fluorescence Assay

Biofilms enable the persistence of pathogens in food processing environments. Sanitizing agents are needed that are effective against pathogens entrapped in biofilms that are more difficult to inactivate than planktonic cells that are displaced and found on equipment surfaces. We examined conditions to develop, analyze, and enumerate the enhanced biofilms of three different foodborne pathogens assisted by fluorescence adherence assay and enzymatic detachment. We compared three different isomeric forms of fluorescent substrates that are readily taken up by bacterial cells based on carboxy-fluorescein diacetate (5-CFDA, 5,6-CFDA, 5,6-CFDA, SE). Biofilm-forming strains of Escherichia coli O157:H7 F4546 and Salmonella Montevideo FSIS 051 were identified using a microplate fluorescence assay defined previously for L. monocytogenes. Adherence levels were determined by differences in relative fluorescence units (RFU) as well as recovered bacterial cells. Multiple hydrolytic enzymes were examined for each representative pathogen for the most suitable enzyme for detachment and enumeration to confirm adherence data obtained by fluorescence assay. Cultures were grown overnight in microplates, incubated, washed and replenished with fresh sterile growth medium; this cycle was repeated for seven consecutive days to enrich for robust biofilms. Treatments were performed in triplicate and compared by one-way analysis of variance (ANOVA) to determine significant differences (p < 0.05).

scholarly journals Optimization of an Extended Microplate Assay for Generating Listeria monocytogenes, E. coli O157:H7, and Salmonella Biofilms and Enzymatic Recovery for Enumeration

2019 ◽  
Author(s):  
Manish Aryal ◽  
Preetty Pranatharthiharan ◽  
Peter M. Muriana
Keyword(s):  
Foodborne Pathogens ◽  
Food Processing ◽  
Hydrolytic Enzymes ◽  
Extracellular Polysaccharide ◽  
Fluorescence Assay ◽  
Bacterial Cells ◽  
Microplate Assay ◽  
Planktonic Cells ◽  
E Coli ◽  
Microplate Fluorescence Assay

Biofilms enable the persistence of pathogens in food processing environments. In order to inactivate these microorganisms, sanitizing agents are needed that are effective against pathogens entrapped in biofilms which are more difficult to inactivate than planktonic cells that are displaced and found on equipment surfaces. We examined conditions to develop, analyze, and enumerate robust biofilms of 3 different foodborne pathogens assisted by fluorescence adherence assay and enzymatic detachment. We compared 3 different isomeric forms of fluorescent substrates that are readily taken up by bacterial cells based on carboxy-fluorescein diacetate (5-CFDA, 5,6-CFDA, 5,6-CFDA,SE). Biofilm-forming strains of L. monocytogenes, E.coli O157:H7, and Salmonella serovars were detected using the chosen substrate in a microplate fluorescence assay define previously for use with Listeria. Adherence levels were determined by differences in relative fluorescence units (RFU). Multiple hydrolytic enzymes were examined for each representative pathogen for the most suitable enzyme for detachment and enumeration to confirm data obtained by fluorescence assay. Cultures were grown overnight in microplates, incubated, washed and replenished with fresh sterile growth medium; this cycle was repeated 7 times before used as &lsquo;biofilms&rsquo;. All treatments were performed in triplicate and compared by one-way analysis of variance (ANOVA) to determine significant differences (p &lt; 0.05). Analysis of 7-day biofilms by SEM indicated possible extracellular polysaccharide involvement with Salmonella and E. coli.

Aptamer-Based Fluorescence Assay for Hg2+ Determination

2013 ◽  
Vol 40 (12) ◽  
pp. 1827-1831
Author(s):  
Zhao-Jing LEI ◽  
Cun-Zheng ZHANG ◽  
Qiu-Hui HU ◽  
Yuan LIU ◽  
Qiang ZHANG ◽  
...  
Keyword(s):  
Fluorescence Assay

Label-free and ultrasensitive fluorescence assay for Fe3+ detection using DNA-Templated Ag nanoclusters

2019 ◽  
Vol 579 ◽  
pp. 123656 ◽  
Author(s):  
Zhiqing Zhang ◽  
Tingting Liu ◽  
Changle Yue ◽  
Shanshan Wang ◽  
Jie Ma ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Label Free ◽  
Ag Nanoclusters

Facile and Sensitive Fluorescence Assay of DNA Polymerase Activity Using Cu2+ and Ascorbate as Signal Developers

2019 ◽  
Vol 4 (8) ◽  
pp. 2398-2403 ◽  
Author(s):  
Xingxing Zhang ◽  
Qiang Liu ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Fluorescence Assay
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