On-chip microelectrophoresis for the study of in vitro nonhomologous end-joining DNA double-strand break repair

2012 ◽  
Vol 425 (1) ◽  
pp. 76-79 ◽  
Author(s):  
Catherine Charles ◽  
Moustapha Ouedraogo ◽  
Alexandra Belayew ◽  
Pierre Duez
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Nonhomologous End Joining ◽  
Double Strand Break Repair ◽  
End Joining ◽  
Dna Double Strand Break ◽  
Break Repair ◽  
On Chip

scholarly journals Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase

2003 ◽  
Vol 23 (7) ◽  
pp. 2309-2315 ◽  
Author(s):  
Stephanie A. Nick McElhinny ◽  
Dale A. Ramsden
Keyword(s):  
High Specificity ◽  
Strand Break ◽  
Double Strand Break ◽  
Nonhomologous End Joining ◽  
Double Strand Break Repair ◽  
End Joining ◽  
Strand Breaks ◽  
Break Repair ◽  
The Impact

ABSTRACT DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity. We show here that polymerase mu (pol μ), implicated in the nonhomologous end-joining pathway for repair of DNA double-strand breaks, incorporates both ribonucleotides and deoxynucleotides in a template-directed manner. pol μ has an approximately 1,000-fold-reduced ability to discriminate against ribonucleotides compared to that of the related pol β, although pol μ's substrate specificity is similar to that of pol β in most other respects. Moreover, pol μ more frequently incorporates ribonucleotides when presented with nucleotide concentrations that approximate cellular pools. We therefore addressed the impact of ribonucleotide incorporation on the activities of factors required for double-strand break repair by nonhomologous end joining. We determined that the ligase required for this pathway readily joined strand breaks with terminal ribonucleotides. Most significantly, pol μ frequently introduced ribonucleotides into the repair junctions of an in vitro nonhomologous end-joining reaction, an activity that would be expected to have important consequences in the context of cellular double-strand break repair.

scholarly journals Bcl2 Negatively Regulates DNA Double-Strand-Break Repair through a Nonhomologous End-Joining Pathway

2008 ◽  
Vol 29 (4) ◽  
pp. 488-498 ◽  
Author(s):  
Qinhong Wang ◽  
Fengqin Gao ◽  
W. Stratford May ◽  
Yangde Zhang ◽  
Tammy Flagg ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Nonhomologous End Joining ◽  
Double Strand Break Repair ◽  
End Joining ◽  
Dna Double Strand Break ◽  
Break Repair
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