A functional dual-coated (FDC) microtiter plate method to replace the botulinum toxin LD50 test

2012 ◽  
Vol 425 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Yvonne Y.B. Liu ◽  
Peter Rigsby ◽  
Dorothea Sesardic ◽  
James D. Marks ◽  
Russell G.A. Jones
Keyword(s):  
Botulinum Toxin ◽  
Microtiter Plate ◽  
Plate Method ◽  
Ld50 Test

Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method

2017 ◽  
Vol 11 (4) ◽  
pp. 1034-1040 ◽  
Author(s):  
Vesna Župunski ◽  
Mirjana Vasić ◽  
Jelka Šuštar Vozlič ◽  
Marko Maras ◽  
Aleksandra Savić ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Microtiter Plate ◽  
Activity Measurement ◽  
Trypsin Inhibitor Activity ◽  
Inhibitor Activity ◽  
Plate Method ◽  
Legume Crops

scholarly journals Determining the Ability of Various Chelators to Stabilize a Reducing Environment via a Microtiter Plate Method

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Ewa Lang ◽  
Stefan Hershberger ◽  
Shyam Vaidya ◽  
Kevin Rupprecht ◽  
Jeffrey Fishpaugh
Keyword(s):  
Microtiter Plate ◽  
Plate Method ◽  
Reducing Environment

A Modified Microtiter Plate Method for Biochemical Characterization of Listeria spp.

1991 ◽  
Vol 54 (2) ◽  
pp. 121-123 ◽  
Author(s):  
GREGORY R. SIRAGUSA ◽  
JERRY W. NIELSEN
Keyword(s):  
Biochemical Characterization ◽  
Microtiter Plate ◽  
Original Method ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Solid Media ◽  
Us Fda ◽  
Term Storage ◽  
Microtiter Plate Format

A microtiter plate format previously reported for the biochemical characterization of microorganisms was modified by adding agar to all of the different test media instead of using a combination of liquid and solid media. This modification, termed the Modified Microtiter Plate procedure (MMP), offered the same advantages of the original method (labor saving, inexpensive, and custom designed for special needs) as well as having the added advantages of longer term storage prior to use and ease of handling. In this study, 60 bacterial isolates (both Listeria suspects and known Listeria cultures) were biotyped using the MMP protocol and compared to results obtained using the classical tube-based US-FDA recommended protocol as the standard identification method and the BBL-Minitek system.

scholarly journals MICROTITER PLATE METHOD FOR IDENTIFICATION OF PTP1B PHOSPHATASE INHIBITORS

2019 ◽  
Vol 70 (2) ◽  
pp. 81-83
Author(s):  
D.A. Babkov ◽  
◽  
E.V. Sokolova ◽  
A.A. Brigadirova ◽  
O.Yu. Zakharyashcheva ◽  
...  
Keyword(s):  
Microtiter Plate ◽  
Plate Method ◽  
Phosphatase Inhibitors

scholarly journals Antibiofilm Activity of Methanol Extract of Rumex dentatus Against Pseudomonas aeruginosa

2020 ◽  
Vol 1 (1) ◽  
pp. 25-32
Author(s):  
Maryam Pezeshki Najafabadi ◽  
Maryam Mohammadi-Sichani ◽  
Mohammad Javad Kazemi ◽  
Mohammad Sadegh Shirsalimian ◽  
Majid Tavakoli
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Microtiter Plate ◽  
Extracellular Polysaccharides ◽  
Antibiofilm Activity ◽  
Plate Method ◽  
Hospital Acquired Infections ◽  
Hospital Acquired

Biofilm formation of Pseudomonas aeruginosa makes up a sizeable proportion of hospital-acquired infections, because bacteria in biofilms can resist antibiotic treatment. The extracellular polymeric substance of P. aeruginosa biofilm is an imprecise collection of extracellular polysaccharides, proteins and microbial cells. Rumex dentatus belongs to polygonaceae family. This family can be found in Middle East. The aim of this present study was to assess the effect of various concentrations of methanol extract of Rumex dentatus on biofilm formation of Pseudomonas aeruginosa after 48 h and 72 h. In this experimental study we collected Rumex dentatus from Khoramabad, Iran. The working extracts were 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95, 0.97 and 0.48 mg/ml. We used microtiter plate method to grow P. aeruginosa biofilm and assess the antibiofilm activity of plant extract. The composition of methanol extract obtained from Rumex dentatus was studied by gas chromatography. The minimum biofilm inhibitory concentration (MBIC) for P. aeruginosa found to be 250 mg/ml. GC-MS  analyses indicated that these fractions contained a variety of compounds including Bicyclo (3.1.1) heptan- 3 -one, 2, 6, 6- trimethyl,  Bicyclo (3.1.1) heptan, 6, 6- dimethyl and Eucalyptol. There were consequential correlations between antibiofilm activity and the concentration of extracts after 48 and 72 h.

scholarly journals A rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial stocks

1989 ◽  
Vol 17 (22) ◽  
pp. 9498-9498 ◽  
Author(s):  
J.Pual Schofield ◽  
Mark Vaudin ◽  
Susan Kettle ◽  
D. Stephen C. Jones
Keyword(s):  
Microtiter Plate ◽  
Plate Method

Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity

2016 ◽  
Vol 21 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Vesna Župunski ◽  
Zoran Mitrović ◽  
Zorica Nikolić ◽  
Gordana Petrović ◽  
Gordana Tamindžić ◽  
...  
Keyword(s):  
Uncertainty Analysis ◽  
Trypsin Inhibitor ◽  
Microtiter Plate ◽  
Trypsin Inhibitor Activity ◽  
Inhibitor Activity ◽  
Plate Method

Development of a fluorescence-based microtiter plate method for the measurement of glutathione in yeast

Talanta ◽  
2006 ◽  
Vol 70 (4) ◽  
pp. 876-882 ◽  
Author(s):  
Kamil Lewicki ◽  
Stéphanie Marchand ◽  
Lydia Matoub ◽  
Janina Lulek ◽  
Joël Coulon ◽  
...  
Keyword(s):  
Microtiter Plate ◽  
Plate Method
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