Rapid and sensitive determination of the intermediates of advanced glycation end products in the human nail by ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry

2012 ◽  
Vol 424 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Jun Zhe Min ◽  
Makoto Yamamoto ◽  
Hai-fu Yu ◽  
Tatsuya Higashi ◽  
Toshimasa Toyo’oka
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ultra Performance Liquid Chromatography ◽  
Advanced Glycation ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Glycation End Products ◽  
End Products ◽  
Sensitive Determination

scholarly journals Identification of Antiglycative Compounds in Japanese Red Water Pepper (Red Leaf Variant of the Persicaria hydropiper Sprout)

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2319 ◽  
Author(s):  
Wakako Takabe ◽  
Taiki Yamaguchi ◽  
Hideharu Hayashi ◽  
Natsuhiko Sugimura ◽  
Masayuki Yagi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hot Water ◽  
Advanced Glycation ◽  
Flight Mass Spectrometry ◽  
Glycation End Products ◽  
End Products ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Layer Chromatography

Glycation, the nonenzymatic reaction between proteins and excess blood sugar, is implicated in multiple disorders and occurs via the formation and accumulation of advanced glycation end products (AGEs). In our previous studies, we demonstrated that the red-leaf variant of the Persicaria hydropiper sprout (Japanese red water pepper, Benitade) is one of the potent plants that inhibit formation of AGEs. In this study, we aimed to identify antiglycative compounds in Benitade. Benitade extracts were prepared with hot water, then fractionated by using high-performance liquid chromatography (HPLC). The antiglycative efficacy of each fraction was evaluated by measuring the formation of fluorescent AGEs (Ex 370 nm/Em 440 nm). Two fractions, which contained peaks at 26.4 min and 31.8 min, showed potent antiglycative efficacy. When we hydrolyzed these peaks, they shifted to 32.5 and 41.4 min, which are the same retention times as cyanidin and quercetin, respectively. Based on thin-layer chromatography, both compounds contained galactose. Finally, ultrahigh-performance liquid chromatography/quadrupole-time of flight mass spectrometry (UHPLC-QqTOF-MS) analyses were performed to determine the structure of those compounds. Overall, we identified two glycosides, cyanidin 3-O-galactoside (idaein) and quercetin 3-O-galactoside (hyperin), as representative antiglycative compounds in Benitade.

Development of a method for the determination of advanced glycation end products precursors by liquid chromatography and its application in human urine samples

2012 ◽  
Vol 35 (19) ◽  
pp. 2575-2584 ◽  
Author(s):  
María del Carmen Hurtado-Sánchez ◽  
Anunciación Espinosa-Mansilla ◽  
María Isabel Rodríguez-Cáceres ◽  
Elisabet Martín-Tornero ◽  
Isabel Durán-Merás
Keyword(s):  
Liquid Chromatography ◽  
Advanced Glycation End Products ◽  
Human Urine ◽  
Urine Samples ◽  
Advanced Glycation ◽  
Human Urine Samples ◽  
Glycation End Products ◽  
End Products
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