Immobilization of hydrophobic peptidic ligands to hydrophilic chromatographic matrix: A preconcentration approach

2012 ◽  
Vol 423 (2) ◽  
pp. 202-209 ◽  
Author(s):  
Satyen Gautam ◽  
Kai-Chee Loh
Keyword(s):  
Chromatographic Matrix

Adsorption behavior of plasmid DNA onto perfusion chromatographic matrix

2013 ◽  
Vol 30 (5) ◽  
pp. 522-526
Author(s):  
LIMONTA Miladys ◽  
ZUMALACARREGUI Lourdes ◽  
SOLER Dayana
Keyword(s):  
Plasmid Dna ◽  
Adsorption Behavior ◽  
Chromatographic Matrix

Gas Chromatographic/Matrix Isolation/Fourier Transform Infrared Spectra of the Laterally Chlorinated Dibenzo-p-Dioxins and Dibenzofurans

1989 ◽  
Vol 43 (8) ◽  
pp. 1317-1324 ◽  
Author(s):  
Charles J. Wurrey ◽  
Billy J. Fairless ◽  
Harry E. Kimball
Keyword(s):  
Fourier Transform ◽  
Infrared Spectra ◽  
Matrix Isolation ◽  
Fourier Transform Infrared ◽  
Fourier Transform Infrared Spectra ◽  
Reference Quality ◽  
Ft Ir ◽  
Chlorinated Dioxins ◽  
Chromatographic Matrix ◽  
Ft Ir Spectroscopy

Reference-quality gas chromatographic/matrix isolation/Fourier transform infrared spectra have been recorded for the following fifteen compounds, which collectively are referred to as the “laterally” chlorinated dibenzo- p-dioxins and dibenzofurans: 2,3,7,8-tetrachlorodibenzo- p-dioxin (2,3,7,8-TCDD); 1,2,3,7,8-pentachlorodibenzo- p-dioxin (1,2,3,7,8-PeCDD);l,2,3,4,7,8-hexachlorodibenzo- p-dioxin(1,2,3,4,7,8-HxCDD)>; 1,2,3,6,7,8,-hexachlorodibenzo- p-dioxin(1,2,3,6,7,8-HxCDD); 1,2,3,7,8,9-hexachlorodibenzo- p-dioxin (1,2,3,7,8,9-HxCDD); 1,2,3,4,6,7,8-heptachlorodibenzo- p-dioxin (1,2,3,4,6,7,8-HpCDD); 2,3,7,8-tetrachlorodibenzofuran (2,3,7,8-TCDF); 1,2,3,7,8-pentachlorodibenzofuran (1,2,3,7,8-PeCDF); 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PeCDF); 1,2,3,4,7,8-hexachlorodibenzofuran(1,2,3,4,7,8-HxCDF); 1,2,3,6,7,8-hexachlorodibenzofuran (1,2,3,6,7,8-HxCDF); 1,2,3,7,8,9-hexachlorodibenzofuran (1,2,3,7,8,9-HxCDF); 2,3,4,6,7,8-hexachlorodibenzofuran (2,3,4,6,7,8-HxCDF); 1,2,3,4,6,7,8-heptachlorodibenzofnran (1,2,3,4,6,7,8-HpCDF); and 1,2,3,4,7,8,9-heptachlorodibenzofuran (1,2,3,4,7,8,9-HpCDF). These spectra are discussed qualitatively and, for the laterally chlorinated dioxins, are compared to previously recorded gas-phase GC/FT-IR spectra. Estimates of the instrumental detection limits for these compounds, using matrix isolation GC/FT-IR spectroscopy, were found to fall in the mid-to-high picogram and low nanogram ranges.

Egg white lysozyme purification with a chitin–silica-based affinity chromatographic matrix

2010 ◽  
Vol 231 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Federico J. Wolman ◽  
Guillermo J. Copello ◽  
Andrea M. Mebert ◽  
Alexandra M. Targovnik ◽  
María V. Miranda ◽  
...  
Keyword(s):  
Egg White ◽  
Egg White Lysozyme ◽  
Chromatographic Matrix

scholarly journals Development of a low-cost pseudobioaffinity chromatographic matrix for lactoferrin purification from milk or whey

2009 ◽  
Vol 25 ◽  
pp. S175
Author(s):  
F.J. Wolman ◽  
A.A. Navarro Del Cañizo ◽  
D. González Maglio ◽  
O. Cascone
Keyword(s):  
Low Cost ◽  
Chromatographic Matrix

Chromatographic matrix based on hydrogel-coated reticulated polyurethane foams, prepared by gamma irradiation

2017 ◽  
Vol 141 ◽  
pp. 300-311 ◽  
Author(s):  
Mirna L. Sánchez ◽  
Claudia Y. Giménez ◽  
Juan F. Delgado ◽  
Leandro J. Martínez ◽  
Mariano Grasselli
Keyword(s):  
Gamma Irradiation ◽  
Polyurethane Foams ◽  
Chromatographic Matrix

scholarly journals Coding Recognition of the Dose-Effect Interdependence of Small Biomolecules Encrypted on Paired Chromatographic-Based Microassay Arrays

2021 ◽  
Author(s):  
Yifeng Deng ◽  
Zhenpeng Lin ◽  
Yuan Cheng
Keyword(s):  
Constraint Satisfaction Problem ◽  
Screening Strategy ◽  
Research Strategy ◽  
Dose Effect ◽  
Chromatographic Behavior ◽  
Molecular Characteristics ◽  
Preliminary Screening ◽  
Dna Base ◽  
Small Biomolecules ◽  
Chromatographic Matrix

AbstractThe discovery of new small molecular entities in diverse compounds suffers from the lack of a correlation recognition method between bioactivity and molecular characteristics such as DNA base pairing recognition in biomacromolecules. Here, by mapping a sample’s 2DHPTLC fingerprint into microplates to create paired chromatographic-based microassay arrays, on which the microarray-based differentially expressed chromatographic spots are allowed to develop and assemble into a self-consistent array distribution gradient of multiattributes of each chromatographic component’s own, we propose a screening strategy to selfrecognize small biomolecules. Specifically, on the paired chromatographic-based microassay arrays with a grid size approximated to that of the chromatographic spots and the chromatographic matrix removed, bioassays and bioguided LC-ESI-MS tracing are performed naturally, the expressed array distribution gradients of the bioactivity strength vs. the digital-specific quasimolecular ion intensity derived from the same chromatographic component are auto-collimated, and generated a pair of dose-effect interdependent 2D codes encrypted by the chromatographic fingerprint characteristics. Therefore, the recognition of molecules attributed to bioactivity in diverse compounds is transformed into a constraint satisfaction problem, which is addressed through examining the dose-effect interdependence of the 2D code pair by array matching algorithm. This approach represents the paradigm shift in small molecule drug screening from bioassay traversing molecular diversity library itemby- item to self-correlation recognition of each compound’s own multiple attributes through its characteristic chromatographic behavior. This research strategy was successfully applied to galangal, and practiced the high-throughput digital preliminary screening of small biomolecules in a natural product.HighlightMatching of HPTLC-based molecular imprinting and bioautography on microassay arrays Microarray-based differential expression of substance attributes instead of spot scan Array auto-collimation of multi-attributes derived from the same 2D-HPTLC component An array framework for combining phenotype-based and target-based assays with TLC-MS

Visualising fouling of a chromatographic matrix using confocal scanning laser microscopy

2006 ◽  
Vol 95 (4) ◽  
pp. 714-723 ◽  
Author(s):  
Sun Chau Siu ◽  
Rihab Boushaba ◽  
Vithaya Topoyassakul ◽  
Alex Graham ◽  
Sorwar Choudhury ◽  
...  
Keyword(s):  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Chromatographic Matrix
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