Quantification of protein posttranslational modifications using stable isotope and mass spectrometry. II. Performance

2012 ◽  
Vol 421 (2) ◽  
pp. 517-525 ◽  
Author(s):  
Quanzhou Luo ◽  
Jette Wypych ◽  
Xinzhao Grace Jiang ◽  
Xin Zhang ◽  
Shun Luo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Posttranslational Modifications ◽  
Protein Posttranslational Modifications

Quantification of protein posttranslational modifications using stable isotope and mass spectrometry

2012 ◽  
Vol 421 (2) ◽  
pp. 506-516 ◽  
Author(s):  
Xinzhao Grace Jiang ◽  
Izydor Apostol ◽  
Quanzhou Luo ◽  
Jeffrey Lewis ◽  
Ronald Keener ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Posttranslational Modifications ◽  
Protein Posttranslational Modifications

Time-dependent cortisol turnover in tissues using stable isotope tracers and MALDI Mass Spectrometry (MS) sampling

2018 ◽  
Author(s):  
Shazia Khan ◽  
Diego F Cobice ◽  
Dawn EW Livingstone ◽  
C Logan Mackay ◽  
Scott P Webster ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Maldi Mass Spectrometry ◽  
Time Dependent ◽  
Stable Isotope Tracers ◽  
Isotope Tracers

scholarly journals E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Peng Gao ◽  
Xianwei Ma ◽  
Ming Yuan ◽  
Yulan Yi ◽  
Guoke Liu ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
Type I Interferon ◽  
Zinc Fingers ◽  
E3 Ligase ◽  
Type I ◽  
E3 Ligases ◽  
Protein Posttranslational Modifications ◽  
Antiviral Innate Immunity

AbstractUbiquitination is one of the most prevalent protein posttranslational modifications. Here, we show that E3 ligase Nedd4l positively regulates antiviral immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3. Deficiency of Nedd4l significantly impairs type I interferon and proinflammatory cytokine production induced by virus infection both in vitro and in vivo. Nedd4l deficiency inhibits virus-induced ubiquitination of TRAF3, the binding between TRAF3 and TBK1, and subsequent phosphorylation of TBK1 and IRF3. Nedd4l directly interacts with TRAF3 and catalyzes K29-linked ubiquitination of Cys56 and Cys124, two cysteines that constitute zinc fingers, resulting in enhanced association between TRAF3 and E3 ligases, cIAP1/2 and HECTD3, and also increased K48/K63-linked ubiquitination of TRAF3. Mutation of Cys56 and Cys124 diminishes Nedd4l-catalyzed K29-linked ubiquitination, but enhances association between TRAF3 and the E3 ligases, supporting Nedd4l promotes type I interferon production in response to virus by catalyzing ubiquitination of the cysteines in TRAF3.

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