Field flow fractionation for assessing neonatal Fc receptor and Fcγ receptor binding to monoclonal antibodies in solution

2011 ◽  
Vol 414 (1) ◽  
pp. 88-98 ◽  
Author(s):  
Joey Pollastrini ◽  
Thomas M. Dillon ◽  
Pavel Bondarenko ◽  
Robert Y.-T. Chou
Keyword(s):  
Monoclonal Antibodies ◽  
Receptor Binding ◽  
Fc Receptor ◽  
Field Flow Fractionation ◽  
Fcγ Receptor ◽  
Neonatal Fc Receptor ◽  
Flow Fractionation

Impact of IgG2 high molecular weight species on neonatal Fc receptor binding assays

2015 ◽  
Vol 489 ◽  
pp. 25-31 ◽  
Author(s):  
Yuling Zhang ◽  
Abhishek Mathur ◽  
Gwen Maher ◽  
Thomas Arroll ◽  
Robert Bailey
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Receptor Binding ◽  
Fc Receptor ◽  
Binding Assays ◽  
Neonatal Fc Receptor ◽  
High Molecular Weight Species

Radiolabeled IgG antibodies: Impact of various labels on neonatal Fc receptor binding

2019 ◽  
Vol 62 (11) ◽  
pp. 751-757 ◽  
Author(s):  
Martin R. Edelmann ◽  
Hubert Kettenberger ◽  
Alexander Knaupp ◽  
Tilman Schlothauer ◽  
Michael B. Otteneder
Keyword(s):  
Receptor Binding ◽  
Fc Receptor ◽  
Igg Antibodies ◽  
Neonatal Fc Receptor

scholarly journals Neutralizing Monoclonal Antibodies That Target the Spike Receptor Binding Domain Confer Fc Receptor-Independent Protection against SARS-CoV-2 Infection in Syrian Hamsters

mBio ◽  
2021 ◽  
Author(s):  
Wen Su ◽  
Sin Fun Sia ◽  
Aaron J. Schmitz ◽  
Traci L. Bricker ◽  
Tyler N. Starr ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Severe Acute Respiratory Syndrome ◽  
Receptor Binding ◽  
Neutralizing Antibodies ◽  
Fc Receptor ◽  
Spike Protein ◽  
Receptor Binding Domain ◽  
Syrian Hamsters ◽  
Convalescent Phase ◽  
Main Target

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein is the main target for neutralizing antibodies. These antibodies can be elicited through immunization or passively transferred as therapeutics in the form of convalescent-phase sera or monoclonal antibodies (MAbs).

scholarly journals Antibody Fc engineering for enhanced neonatal Fc receptor binding and prolonged circulation half-life

mAbs ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1276-1288 ◽  
Author(s):  
Brian C. Mackness ◽  
Julie A. Jaworski ◽  
Ekaterina Boudanova ◽  
Anna Park ◽  
Delphine Valente ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Half Life ◽  
Fc Receptor ◽  
Neonatal Fc Receptor

scholarly journals Impact of SPR biosensor assay configuration on antibody: Neonatal Fc receptor binding data

mAbs ◽  
2016 ◽  
Vol 9 (2) ◽  
pp. 319-332 ◽  
Author(s):  
Xiangdan Wang ◽  
Patrick McKay ◽  
Liliana T. Yee ◽  
George Dutina ◽  
Philip E. Hass ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Fc Receptor ◽  
Neonatal Fc Receptor ◽  
Spr Biosensor ◽  
Binding Data

Neonatal Fc Receptor Binding Tolerance toward the Covalent Conjugation of Payloads to Cysteine 34 of Human Albumin Variants

2015 ◽  
Vol 13 (2) ◽  
pp. 677-682 ◽  
Author(s):  
Steffan S. Petersen ◽  
Eva Kläning ◽  
Morten F. Ebbesen ◽  
Birgitte Andersen ◽  
Jason Cameron ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Human Albumin ◽  
Fc Receptor ◽  
Neonatal Fc Receptor

scholarly journals Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects

1995 ◽  
Vol 25 (6) ◽  
pp. 1492-1496 ◽  
Author(s):  
Ann C. T. M. Vossen ◽  
G. John M. Tibbe ◽  
Martin J. Kroos ◽  
Jan G. J. van de Winkel ◽  
Robbert Benner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Side Effects ◽  
Receptor Binding ◽  
Fc Receptor

A cell-based FcRn-dependent recycling assay for predictive pharmacokinetic assessment of therapeutic antibodies

Bioanalysis ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 1135-1144
Author(s):  
Chang Liu ◽  
Yeon Su Kim ◽  
John Hok-Nin Lowe ◽  
Shan Chung
Keyword(s):  
Monoclonal Antibodies ◽  
Fc Receptor ◽  
Strongly Correlated ◽  
Therapeutic Antibodies ◽  
Neonatal Fc Receptor ◽  
Pharmacokinetic Assessment ◽  
Pharmacokinetic Properties ◽  
A Cell ◽  
Therapeutic Monoclonal Antibodies ◽  
Selection Of

Aim: Evaluation of suitable pharmacokinetic properties is critical for successful development of IgG-based biotherapeutics. The prolonged half-lives of IgGs depend on the intracellular trafficking function of neonatal Fc receptor, which rescues internalized IgGs from lysosomal degradation and recycles them back to circulation. Results: Here, we developed a novel cell-based assay to quantify recycling of monoclonal antibodies in a transwell culture system that uses a cell line that stably expresses human neonatal Fc receptor. We tested seven therapeutic antibodies and showed that the recycling output of the assay strongly correlated with the clearance in humans. Conclusion: This recycling assay has potential application as a pharmacokinetic prescreening tool to facilitate development and selection of IgG-based candidate therapeutic monoclonal antibodies.

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