Single-step protein purification by back flush in ion exchange chromatography

2009 ◽  
Vol 392 (2) ◽  
pp. 174-176 ◽  
Author(s):  
Ming-Kai Chern ◽  
Wei-Jyh Shiah ◽  
Jyun-Jie Chen ◽  
Tzung-You Tsai ◽  
Hsin-Yin Lin ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Protein Purification ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step

Micro-Plate Based Monolithic Ion-Exchange Chromatography for High Throughput Protein Purification Process Design

2008 ◽  
Vol 41 (3) ◽  
pp. 200-205 ◽  
Author(s):  
Noriko Yoshimoto ◽  
Yuko Nishijima ◽  
Parvin Akbarzadehlaleh ◽  
Sachie Fujii ◽  
Mitsuyo Abe ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Protein Purification ◽  
High Throughput ◽  
Process Design ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Purification Process

scholarly journals Current Trends in Protein Purification : A Review

2020 ◽  
pp. 279-310
Author(s):  
Angela Boxi ◽  
Isha Parikh ◽  
Radhika B S ◽  
Shryli K S
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Affinity Chromatography ◽  
Protein Purification ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Current Trends ◽  
Comparative Information

The present review is based on papers published between 1990 and 2020 and gives Comparative information about the most common protein purification techniques Gel-Filtration, Chromatography, Ion-Exchange Chromatography, Electrophoresis, Affinity Chromatography, and Dialysis, High-Pressure Liquid Chromatography. and their applications.

scholarly journals A rapid and simple method for the purification of transglutaminase from Streptoverticillium mobaraense.

1994 ◽  
Vol 299 (3) ◽  
pp. 825-829 ◽  
Author(s):  
U Gerber ◽  
U Jucknischke ◽  
S Putzien ◽  
H L Fuchsbauer
Keyword(s):  
Ion Exchange ◽  
Storage Stability ◽  
Culture Fluid ◽  
Strong Acid ◽  
Weak Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step ◽  
Simple Method ◽  
High Yields

Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.

Alkyl Based Selective Displacers for Protein Purification in Ion Exchange Chromatography

2010 ◽  
Vol 72 (11-12) ◽  
pp. 1025-1034
Author(s):  
Christopher J. Morrison ◽  
J. A. Moore ◽  
Steven M. Cramer
Keyword(s):  
Ion Exchange ◽  
Protein Purification ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography

Expanded and fixed bed ion exchange chromatography for the recovery of C-phycocyanin in a single step by using lysed cells

2014 ◽  
Vol 93 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Caroline Costa Moraes ◽  
Luisa Sala ◽  
Joana da Costa Ores ◽  
Anna Rafaela Cavalcante Braga ◽  
Jorge Alberto Vieira Costa ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Fixed Bed ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step
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