High-throughput chemiluminometric genotyping of single nucleotide polymorphisms of histamine, serotonin, and adrenergic receptor genes

2009 ◽  
Vol 385 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Dimitra K. Toubanaki ◽  
Theodore K. Christopoulos ◽  
Penelope C. Ioannou ◽  
Christodoulos S. Flordellis
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Throughput ◽  
Adrenergic Receptor ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Single-Nucleotide Polymorphisms for High-Throughput Genotyping of Anopheles arabiensis in East and Southern Africa

2012 ◽  
Vol 49 (2) ◽  
pp. 307-315 ◽  
Author(s):  
Yoosook Lee ◽  
Stephanie N. Seifert ◽  
Christen M. Fornadel ◽  
Douglas E. Norris ◽  
Gregory C. Lanzaro
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Throughput ◽  
Southern Africa ◽  
Anopheles Arabiensis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Analytical Validation of the Tag-It High-Throughput Microsphere-Based Universal Array Genotyping Platform: Application to the Multiplex Detection of a Panel of Thrombophilia-Associated Single-Nucleotide Polymorphisms

2004 ◽  
Vol 50 (11) ◽  
pp. 2028-2036 ◽  
Author(s):  
Susan Bortolin ◽  
Margot Black ◽  
Hemanshu Modi ◽  
Ihor Boszko ◽  
Daniel Kobler ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Throughput ◽  
Nucleotide Polymorphisms ◽  
Sequencing Data ◽  
Single Nucleotide ◽  
Direct Dna Sequencing ◽  
Array Platform ◽  
Genotyping Platform ◽  
Universal Array ◽  
Primer Sets

Abstract Background: We have developed a novel, microsphere-based universal array platform referred to as the Tag-It™ platform. This platform is suitable for high-throughput clinical genotyping applications and was used for multiplex analysis of a panel of thrombophilia-associated single-nucleotide polymorphisms (SNPs). Methods: Genomic DNA from 132 patients was amplified by multiplex PCR using 6 primer sets, followed by multiplex allele-specific primer extension using 12 universally tagged genotyping primers. The products were then sorted on the Tag-It array and detected by use of the Luminex xMAP™ system. Genotypes were also determined by sequencing. Results: Empirical validation of the universal array showed that the highest nonspecific signal was 3.7% of the specific signal. Patient genotypes showed 100% concordance with direct DNA sequencing data for 736 SNP determinations. Conclusions: The Tag-It microsphere-based universal array platform is a highly accurate, multiplexed, high-throughput SNP-detection platform.

scholarly journals 11 Million SNP Reference Profiles for Identity Searching Across Ethnicities, Kinship, and Admixture

2018 ◽  
Author(s):  
Brian S. Helfer ◽  
Darrell O. Ricke
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Throughput ◽  
Family Relationships ◽  
In Silico ◽  
High Throughput Sequencing ◽  
Genetic Data ◽  
Nucleotide Polymorphisms ◽  
Mixture Analysis ◽  
Single Nucleotide ◽  
Public Repositories

AbstractHigh throughput sequencing (HTS) of single nucleotide polymorphisms (SNPs) provides additional applications for DNA forensics including identification, mixture analysis, kinship prediction, and biogeographic ancestry prediction. Public repositories of human genetic data are being rapidly generated and released, but the majorities of these samples are de-identified to protect privacy, and have little or no individual metadata such as appearance (photos), ethnicity, relatives, etc. A reference in silico dataset has been generated to enable development and testing of new DNA forensics algorithms. This dataset provides 11 million SNP profiles for individuals with defined ethnicities and family relationships spanning eight generations with admixture for a panel with 39,108 SNPs.

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