Determination of rat serum esterase activities by an HPLC method using S-acetylthiocholine iodide and p-nitrophenyl acetate

2008 ◽  
Vol 381 (1) ◽  
pp. 113-122 ◽  
Author(s):  
Matthias Koitka ◽  
Joachim Höchel ◽  
Detlev Obst ◽  
Antje Rottmann ◽  
Hille Gieschen ◽  
...  
Keyword(s):  
Hplc Method ◽  
Rat Serum ◽  
Acetylthiocholine Iodide ◽  
Esterase Activities

scholarly journals Determination of Glimepiride in Rat Serum by Rp-Hplc Method

2011 ◽  
Vol 02 (02) ◽  
pp. 152-157 ◽  
Author(s):  
Sujatha Samala ◽  
Sandhya Rani Tatipamula ◽  
Ciddi Veeresham
Keyword(s):  
Hplc Method ◽  
Rat Serum ◽  
Rp Hplc

scholarly journals Development of alternative HPLC method for determination of tirofiban in rat serum

2016 ◽  
Vol 35 (2) ◽  
pp. 217
Author(s):  
Marija Darkovska-Serafimovska ◽  
Emilija Janevik-Ivanovska ◽  
Trajan Balkanov ◽  
Nenad Ugresic
Keyword(s):  
Orthophosphoric Acid ◽  
Hplc Method ◽  
Control Group ◽  
Reverse Phase Hplc ◽  
Rat Serum ◽  
Coronary Syndrome ◽  
Rp Hplc ◽  
Fibrinogen Binding ◽  
Water Ph

<p>Tirofiban hydrochloride is a reversible antagonist of fibrinogen binding to the GPIIb/IIIa receptor, used for the treatment of acute coronary syndrome. A novel RP-HPLC method has been developed and validated for the determination of Tirofiban in serum of Wistar rats with and without deep acute venous thrombosis. The chromatographic separation was carried out using a reverse-phase HPLC column Purospher® RP-18e (150 mm ´ 4.6 mm i.d.; 5 μm) coupled with a guard column LiChrosorb® (4 mm ´ 4 mm i.d.; 7 μm) and mobile phase consisting of the mixture of 1-octane sulfonic acid in water (pH 3.0, adjusted with orthophosphoric acid) and acetonitrile, with a ratio of 60 : 40 (<em>v/v</em>) and a flow rate of 1.0 ml/min, at a wavelength of 277 nm. The serum concentrations of Tirofiban in the group of rats with DVT were lower than those in the control group, and it could be explained with the binding of Tirofiban with the GPIIb/IIIa receptors. </p>

RP-HPLC method development for simultaneous determination of the drugs Ramipril and Amlodipine

2012 ◽  
Vol 2 (2) ◽  
pp. 364-367 ◽  
Author(s):  
Saida Naik Dheeravath ◽  
◽  
Kasani Ramadevi ◽  
Zilla Saraswathi ◽  
Dheeravath Maniklal ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Hplc Method ◽  
Rp Hplc ◽  
Hplc Method Development

A comparative technique using as Joint studying to prove structure and determination the Quantitative estimation of organic compound (Irbesartan) as drug in multicomponent tablet form

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Imad Tarek Hanoon ◽  
Abed Mohammed Daheir AL-Joubory 2 ◽  
Marwa Mohamed Saied 3
Keyword(s):  
Mobile Phase ◽  
Chemical Structure ◽  
Quantitative Estimation ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Pharmaceutical Dosage Forms ◽  
Tablet Form ◽  
Rp Hplc ◽  
Percent Recovery

A simple , specific, accurate and precise RP-HPLC method was developed for determination of Irbesartan (IRB) in pharmaceutical dosage forms in tablets products and sachet using symmetry (L 1 ) column at 30°C . The signal was detected at 225 nm. A mobile phase dissolve 0.5 g of buffer potassium phosphate in 100 ml distilled water and adjust pH 2.7 , methanol and acetonitrile at ratio (40 :30 :30 ) . and flow rate 1.2ml/min -1 at pH=7.2 a mobile phase The percent recovery was detected 101 % and the linearity of concentration was 10-50 µg.ml -1 and supported this method by using (FT.I.R.) spectrum method for organic spectrophotometer to prove the chemical structure of this drug and some physical properties . we are obtained the result is identical of other literature . The proposed method was applied successfully for determination of the IRB in tablets products.

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