Clonal polymerase chain reaction–single-strand conformational polymorphism analysis: An effective approach for identifying cloned sequences

2008 ◽  
Vol 378 (1) ◽  
pp. 111-112 ◽  
Author(s):  
H. Zhou ◽  
J.G.H. Hickford
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Strand ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Polymerase Chain

A preliminary survey of mitochondrial sequence variation in Triatominae (Hemiptera: Reduviidae) using polymerase chain reaction-based single strand conformational polymorphism (SSCP) analysis and direct sequencing

1998 ◽  
Vol 88 (5) ◽  
pp. 553-560 ◽  
Author(s):  
J. R. Stothard ◽  
Y. Yamamoto ◽  
A. Cherchi ◽  
A. L. Garcia ◽  
S. A. S. Valente ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sequence Variation ◽  
Direct Sequencing ◽  
Single Strand ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Polymerase Chain ◽  
Selection Of Species

AbstractGenetic variation within triatomine bugs was investigated by amplification of a 400 bp portion of the mitochondrial 16S ribosomal RNA gene by polymerase chain reaction (PCR), using evolutionarily conserved primers, from a selection of species representative of the genera Rhodnius, Triatoma and Panstrongylus. Amplification products were subsequently screened for sequence variation using single strand conformational polymorphism analysis (SSCP) and also subjected to direct sequencing. Single strand conformational polymorphism analysis could detect variation within and between genera; intraspecific variation was also detected and SSCP profiles appear to be useful for identification purposes at the inter- and intraspecific levels. A 290 bp multiple alignment of 15 sequences obtained from nine species was generated, phylogenetic inference subsequently used three methods; a distance estimate, maximum parsimony and maximum likelihood. This 16S region exhibited considerable variation which ranged from intergeneric to intraspecific levels. Phylogenies from these three methods of inference were in broad agreement. Triatoma and Panstrongylus were more closely related to each other than either was to Rhodnius, in keeping with the current taxonomic appraisal.

scholarly journals Application of Polymerase Chain Reaction–Single Strand Conformational Polymorphism (PCR–SSCP) to Identification of Flatfish Species

1999 ◽  
Vol 82 (4) ◽  
pp. 903-907 ◽  
Author(s):  
Ana Céspedes ◽  
Teresa García ◽  
Esther Carrera ◽  
Isabel González ◽  
Esther Carrera ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Single Strand ◽  
Chain Reaction ◽  
Greenland Halibut ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Flatfish Species ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain

Abstract A method of DNA analysis based on polymerase chain reaction–single strand conformational polymorphism (PCR–SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by native polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).

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