Analysis and interpretation of two-dimensional single-particle tracking microscopy measurements: Effect of local surface roughness

2008 ◽  
Vol 377 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Damien Hall
Keyword(s):  
Surface Roughness ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Two Dimensional ◽  
Local Surface

scholarly journals Single-particle tracking discloses binding-mediated rocking diffusion of rod-shaped biological particles on lipid membranes

2019 ◽  
Vol 10 (5) ◽  
pp. 1351-1359 ◽  
Author(s):  
Zhongju Ye ◽  
Hua Liu ◽  
Fuyan Wang ◽  
Xin Wang ◽  
Lin Wei ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Single Particle Tracking ◽  
Two Dimensional ◽  
Biological Particles

Interestingly, on the lipid membrane, E.coli displays anomalous rocking diffusion, which might facilitate the subsequent survey of stronger association sites on the two-dimensional (2D) surface.

scholarly journals Two-Dimensional and Three-Dimensional Single Particle Tracking of Upconverting Nanoparticles in Living Cells

2019 ◽  
Vol 20 (6) ◽  
pp. 1424 ◽  
Author(s):  
Kyujin Shin ◽  
Yo Song ◽  
Yeongchang Goh ◽  
Kang Lee
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Near Infrared ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Living Cells ◽  
Single Particle Tracking ◽  
Infrared Light ◽  
Objective Lens ◽  
Two Dimensional

Lanthanide-doped upconversion nanoparticles (UCNPs) are inorganic nanomaterials in which the lanthanide cations embedded in the host matrix can convert incident near-infrared light to visible or ultraviolet light. These particles are often used for long-term and real-time imaging because they are extremely stable even when subjected to continuous irradiation for a long time. It is now possible to image their movement at the single particle level with a scale of a few nanometers and track their trajectories as a function of time with a scale of a few microseconds. Such UCNP-based single-particle tracking (SPT) technology provides information about the intracellular structures and dynamics in living cells. Thus far, most imaging techniques have been built on fluorescence microscopic techniques (epifluorescence, total internal reflection, etc.). However, two-dimensional (2D) images obtained using these techniques are limited in only being able to visualize those on the focal planes of the objective lens. On the contrary, if three-dimensional (3D) structures and dynamics are known, deeper insights into the biology of the thick cells and tissues can be obtained. In this review, we introduce the status of the fluorescence imaging techniques, discuss the mathematical description of SPT, and outline the past few studies using UCNPs as imaging probes or biologically functionalized carriers.

scholarly journals Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy

Entropy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 498
Author(s):  
Chen Zhang ◽  
Kevin Welsher
Keyword(s):  
Fisher Information ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Samples ◽  
Tracking System ◽  
Single Particle Tracking ◽  
Uniform Sampling ◽  
Single Dimension ◽  
3D Localization ◽  
Particle Localization

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.

pH-Mediated nanoparticle dynamics in hydrogel nanocomposites

Soft Matter ◽  
2021 ◽  
Author(s):  
Katie A. Rose ◽  
Daeyeon Lee ◽  
Russell J. Composto
Keyword(s):  
Quantum Dot ◽  
Ethylene Glycol ◽  
Particle Tracking ◽  
Single Particle ◽  
Silica Particles ◽  
Single Particle Tracking ◽  
Poly Ethylene Glycol ◽  
Hydrogel Nanocomposites ◽  
Poly Ethylene ◽  
Nanoparticle Dynamics

The effect of static silica particles on the dynamics of quantum dot (QD) nanoparticles grafted with a poly(ethylene glycol) (PEG) brush in hydrogel nanocomposites is investigated using single particle tracking (SPT).

Sign in / Sign up

Export Citation Format

Share Document