Design and characterization of bivalent Smac-based peptides as antagonists of XIAP and development and validation of a fluorescence polarization assay for XIAP containing both BIR2 and BIR3 domains

2008 ◽  
Vol 374 (1) ◽  
pp. 87-98 ◽  
Author(s):  
Zaneta Nikolovska-Coleska ◽  
Jennifer L. Meagher ◽  
Sheng Jiang ◽  
Steven A. Kawamoto ◽  
Wei Gao ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Fluorescence Polarization Assay ◽  
Development And Validation

scholarly journals Characterization of the interactions of chemically-modified therapeutic nucleic acids with plasma proteins using a fluorescence polarization assay

2018 ◽  
Vol 47 (3) ◽  
pp. 1110-1122 ◽  
Author(s):  
Hans J Gaus ◽  
Ruchi Gupta ◽  
Alfred E Chappell ◽  
Michael E Østergaard ◽  
Eric E Swayze ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Polarization ◽  
Plasma Proteins ◽  
Chemically Modified ◽  
Fluorescence Polarization Assay

A Simple, Green and Fast Ultraviolet Spectrophotometric Method for the Carbamide Peroxide Determination in Dental Whitening Products

2019 ◽  
Vol 15 (2) ◽  
pp. 138-144
Author(s):  
Fabiana Vieira Lima ◽  
Aline Farias ◽  
Cassiana Mendes ◽  
Simone Gonçalves Cardoso ◽  
Marcos Antônio Segatto Silva
Keyword(s):  
Hydrogen Peroxide ◽  
Pharmaceutical Products ◽  
Reference Solution ◽  
Iodometric Titration ◽  
Carbamide Peroxide ◽  
Ultraviolet Detection ◽  
Water As Solvent ◽  
Development And Validation ◽  
Dental Whitening

Background: The carbamide peroxide is the most commonly active ingredient used for home dental whitening products, its quantification in pharmaceutical products is of extreme importance due to the relation with the products potency and the previously related low carbamide peroxide stability. Once, there is only one official carbamide peroxide determination based on iodometric titration, this method is time-consuming and generates a lot of residues. The aim of this study was to carry out development and validation of a simple and fast ultraviolet spectrophotometer assay to quantify an innovative dental whitening gel. Methods: The proposed method was validated according international conference on harmonization guideline. Procedure is based on the iodide/iodine redox chemistry; iodine released through the action of hydrogen peroxide of carbamide peroxide with ultraviolet detection at 350 nm. Results: The procedure was linear in the concentration range of 1.0-4.0 µg/mL, specific to the excipients, robust for the evaluated parameters (variation of wavelength (± 5 nm); reagent addition (± 10%)), showing the results of RSD 1.88 and 0.39% respectively. Repeatability precision was RSD = 1.42%, with accurate RSD = 2.15% by adding reference solution. The assay used only water as solvent for sample preparation. In comparison to the pharmacopeial method, the latter is more time-consuming, as it generates a lot of residues, and it could not quantify small CP dosages. Conclusion: Thus, the proposed method was proved to be suitable to determine carbamide peroxide during the development and characterization of nanoparticle formulations in the present study.

Development of a fluorescence polarization assay to screen for inhibitors of the FtsZ/ZipA interaction

2003 ◽  
Vol 323 (2) ◽  
pp. 224-233 ◽  
Author(s):  
Cynthia Hess Kenny ◽  
Weidong Ding ◽  
Kerry Kelleher ◽  
Susan Benard ◽  
Elizabeth Glasfeld Dushin ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Fluorescence Polarization Assay

A Fluorescence Polarization Assay for Cyclic Nucleotide Phosphodiesterases

2002 ◽  
Vol 7 (3) ◽  
pp. 215-222 ◽  
Author(s):  
Wei Huang ◽  
Yan Zhang ◽  
J. Richard Sportsman
Keyword(s):  
Cyclic Nucleotide ◽  
Fluorescence Polarization ◽  
Drug Targets ◽  
Second Messengers ◽  
Reaction Products ◽  
Cyclic Nucleotide Phosphodiesterases ◽  
Extracellular Signals ◽  
Fluorescence Polarization Assay ◽  
Hydrolysis Of ◽  
Nucleotide Phosphodiesterases

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 3′-ester bond of cyclic AMP (cAMP) and cyclic GMP (cGMP), important second messengers in the transduction of a variety of extracellular signals. There is growing interest in the study of PDEs as drug targets for novel therapeutics. We describe the development of a homogeneous fluorescence polarization assay for PDEs based on the strong binding of PDE reaction products (i.e., AMP or GMP) onto modified nanoparticles through interactions with immobilized trivalent metal cations. This assay technology (IMAP) is applicable to both cAMP- and cGMP-specific PDEs. Results of the assay in 384- and 1536-well microplates are presented.

scholarly journals Design, Synthesis, and Characterization of a Fluorescence Polarization Pan-BET Bromodomain Probe

2018 ◽  
Vol 9 (12) ◽  
pp. 1223-1229 ◽  
Author(s):  
Carolyn N. Paulson ◽  
Xianghong Guan ◽  
Alex M. Ayoub ◽  
Alice Chan ◽  
Rezaul M. Karim ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Synthesis And Characterization ◽  
Design Synthesis
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