Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis

2008 ◽  
Vol 373 (2) ◽  
pp. 398-400 ◽  
Author(s):  
Xiangdong Li ◽  
Yafeng Qiu ◽  
Yang Shen ◽  
Chan Ding ◽  
Peihong Liu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction-Based Site-Directed Mutagenesis Using Magnetic Beads

1996 ◽  
Vol 234 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Jacques Fieschi ◽  
Patricia Niccoli ◽  
Christophe Camilla ◽  
Patrick Chames ◽  
Martine Chartier ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Magnetic Beads ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Chain Reaction ◽  
Polymerase Chain

Detecting multiple cystic fibrosis mutations by polymerase chain reaction-mediated site-directed mutagenesis

1991 ◽  
Vol 37 (5) ◽  
pp. 753-755 ◽  
Author(s):  
K J Friedman ◽  
W E Highsmith ◽  
L M Silverman
Keyword(s):  
Cystic Fibrosis ◽  
Polymerase Chain Reaction ◽  
Restriction Enzyme ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Chain Reaction ◽  
Allele Specific ◽  
Specific Restriction ◽  
Polymerase Chain

Abstract The polymerase chain reaction (PCR) has been applied in a novel manner to detect the multiple mutations causing cystic fibrosis (CF). PCR-mediated site-directed mutagenesis (PSM) has been applied to create allele-specific restriction enzyme cutting sites for three of the more common mutations. Two other mutations after cutting sites on their own. We discuss the implications for the expedient detection of five different CF-causing mutations.

scholarly journals A simple method for site-directed mutagenesis using the polymerase chain reaction

1989 ◽  
Vol 17 (16) ◽  
pp. 6545-6551 ◽  
Author(s):  
Anne Hemsley ◽  
Norman Arnheim ◽  
Michael Dennis Toney ◽  
Gino Cortopassi ◽  
David J. Galas
Keyword(s):  
Polymerase Chain Reaction ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Chain Reaction ◽  
Simple Method ◽  
Polymerase Chain
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